A kind of storage method of Hemerocallis pollen

Abstract

The invention discloses a storage method for Hemerocallis pollen, which comprises the following steps: (1) Collecting pollen: The collection time is 7-8 o'clock in the morning when Hemerocallis is in full bloom, gently poking the petals with tweezers, and collecting them with cotton swabs Put the pollen in a centrifuge tube and store it airtight at room temperature without direct light; (2) Determination of pollen viability: use TTC staining method, put a little pollen on a clean glass slide, add TTC solution dropwise, stir After uniformity, cover with a cover glass, put it in a 30-35°C incubator, and inspect under the microscope after 30-35 minutes. If the pollen that is dyed is red, it means that the vitality is strong, and if the pollen is yellow or colorless, it is non-viable or sterile pollen; Observe the above slides, take the staining rate of the pollen as its vitality index, record and count the pollen vitality value; (3) draw a conclusion: the daylily Hemerocallis pollen stored within 7 days should be stored in a refrigerator with a storage temperature of 4°C-6°C For storage, the pollen stored for more than 7 days should be stored in an ultra-low temperature refrigerator at a storage temperature of -75°C -70°C.

Description

technical field [0001] The invention relates to a method for storing pollen, in particular to a method for storing Hemerocallis pollen. Background technique [0002] Hemerocallis fulva (Hemerocallis fulva) is native to Asian and European countries. There are about 14 species of Hemerocallis in the world, and 11 species are native to my country. It is a plant of the genus Hemerocallis in the family Liliaceae. , not directly edible, will grow slender green flowering branches during the flowering period. The flower type is mostly trumpet-shaped, rich in color, and the overall plant shape is relatively tall and straight. Hemerocallis has been cultivated in my country for more than 3,000 years, and it is called "mother flower" and "forgetful grass" in ancient Chinese culture. Because of its rich germplasm resources and wide range of functions, it can be "viewed as a flower, eaten as a vegetable, and used as a medicine", and the current market demand for it is increasing. The He...

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Problems solved by technology

[0003] Currently, the pollen storage method of Hemerocallis pollen is not perfect, and the identification method of pollen vigor is not clear, and there are the following problems: Hemerocallis is widely distributed and has many varieties, and the flowering period is concentrated in May-September, but the flowering period of different varieties is inconsistent. In the process of Hemerocallis hybrid breeding, often If there is a difference of more than 1 month between the flowering periods of the two varieties, or the problem of cross-breeding is involved, the collected pollen needs to be stored or transported. Under normal temperature, the vitality of daylily pollen cannot be maintained for one to two months. If there is no proper storage method, It will cause a lot of waste, especially when pollination is not possible in rainy days. In these processes, the vigor of pollen is affected to a certain extent, which further affects the success rate of hybridization, and cannot meet the requirements of pollen concentration in the process of daylily hybridization and breeding. needs

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