Isotopic methods for the measurement of tryptophan and its metabolites

A technology of tryptophan, metabolites, applied in the direction of measuring devices, labels used in chemical analysis, biological tests, etc.

Active Publication Date: 2022-03-04
ELECTROPHORETICS LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] While inhibition of tryptophan degradation has been studied as a means of suppressing immune evasion in cancer, induction of tryptophan degradation has been implicated in the control of autoimmune disease (6), excessive inflammatory disease tolerance (7), chronic infection and allergy

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  • Isotopic methods for the measurement of tryptophan and its metabolites
  • Isotopic methods for the measurement of tryptophan and its metabolites
  • Isotopic methods for the measurement of tryptophan and its metabolites

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Embodiment

[0149] Certain aspects and embodiments of the invention will now be illustrated by way of example and with reference to the above figures and tables. All documents mentioned in this specification are hereby incorporated by reference in their entirety for all purposes.

[0150] Materials and methods

[0151] Cell Cultures and Reagents

[0152] In supplemented with the indicated L-tryptophan (Sigma-Aldrich, Taufkirchen, Germany), 300 mg / l glutamine (Carl Roth, Karlsruhe, Germany), 10% FBS (Thermo Fisher Scientific Inc., Waltham, MA, USA ) and 100 U / mL penicillin and 100 μg / mL streptomycin (PAA Laboratories, Pasching, Australia) in McCoy's 5A medium (BioConcept, Allschwil, Switzerland) to culture SKOV-3 ovarian cancer cells. Human embryonic kidney (HEK) cells were cultured in DMEM (PAA Laboratories), 10% FBS (Thermo Scientific), and 100 U / mL penicillin and 100 μg / mL streptomycin (PAA Laboratories). Peripheral blood mononuclear cells (PBMC) were isolated from healthy unrelate...

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Abstract

Tryptophan degradation is a key metabolic pathway that controls immune responses, and evidence suggests that during cancer progression, the production of tryptophan metabolites may underlie immune evasion that promotes the malignant phenotype of cancer cells in an autocrine manner. The present invention relates to methods of measuring mass tag-labeled tryptophan and its metabolites, and the use of labeled molecules to monitor the effectiveness of a subject's treatment and disease recurrence following treatment, for stratifying patients and for diagnosing immunity in a subject method of response suppression.

Description

technical field [0001] The present invention relates to methods of measuring mass label-labeled tryptophan and its metabolites, and using the labeled molecules to monitor the effectiveness of treatment and post-treatment disease recurrence in subjects, for stratifying patients and diagnosing in subjects Methods of Suppression of Immune Responses. Background technique [0002] The recent definition of "evading immune destruction" as a new hallmark of cancer reflects a growing recognition of immune suppression and evasion as key features of malignancy (1). Over the past few years, tryptophan degradation has emerged as a key metabolic pathway controlling immune responses. Emerging evidence suggests that during cancer progression, the enzymes indoleamine-2,3-dioxygenase (IDO) and / or tryptophan-2,3-dioxygenase ( Kynurenine production by TDO) may represent a central pathway for immune evasion, while also promoting the malignant phenotype of cancer cells in an autocrine manner (2...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6848G01N33/532G01N2458/15
Inventor S·康卡雷维奇K·库恩P·舒尔茨-克纳佩I·H·派克C·奥皮茨M·普拉滕
Owner ELECTROPHORETICS LTD
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