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A rapid screening method for living organisms against Trichoderma

A screening method and technology for Trichoderma, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of low re-screening efficiency of antagonistic Trichoderma, slow re-screening speed of antagonistic Trichoderma, and the like. The effect of re-screening is that the workload is large, the material is saved, and the screening efficiency is improved

Active Publication Date: 2021-07-13
SHANGHAI JIAOTONG UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a fast screening method for live antagonistic Trichoderma to solve the problem of slow re-screening of antagonistic Trichoderma for the low efficiency of traditional antagonistic Trichoderma re-screening

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  • A rapid screening method for living organisms against Trichoderma
  • A rapid screening method for living organisms against Trichoderma
  • A rapid screening method for living organisms against Trichoderma

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Embodiment 1

[0056] This embodiment relates to a rapid screening method for antagonizing Trichoderma living organisms, comprising the following steps:

[0057]1. Cultivate cucumber anthracnose bacteria (gifted by Li Baoju, a researcher at the Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) on the PDA plate for 7 days. Take the fungus cake from the edge of the colony, transfer it to PD liquid medium, and cultivate it in a constant temperature shaker at 28°C and 180r / min for 5 days. Transfer the cultured fermentation broth to a centrifuge tube, and centrifuge at 10000rr for 10min in a centrifuge to obtain the germ fermentation supernatant.

[0058] 2. In a 5ml centrifuge tube, mix the supernatant of the bacterial fermentation broth with MS medium at a ratio of 1:4, with a total volume of 4ml, to obtain the bacterial inoculum.

[0059] 3. Put the cucumber seeds (Xiafeng No. 2) in a petri dish covered with moist filter paper, and germinate them in a constant temp...

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Abstract

The present invention provides a kind of rapid screening method of antagonizing Trichoderma living body, comprising the following steps: A. Putting the germinated plant seeds in the Trichoderma suspension and soaking them for a certain period of time, sucking up the Trichoderma suspension attached to the surface of the plant seeds B, the radicle of the plant seed treated in step A is placed downwards in the pathogenic bacteria inoculum, cultivated under constant temperature light, and emerge; C, after the area S1 of the cotyledon of the plant after emergence is scanned, it is not inoculated with the control group The cotyledon area S2 of the pathogen and Trichoderma was compared. The greater the ratio, the stronger the antagonism of the corresponding Trichoderma, and thus the Trichoderma strain with excellent antagonism was screened. The invention screens the antagonistic trichoderma by adopting a multi-hole plate combined with a colony area scanner, and the screening result of the method has a high correlation with the screening result of the potted plant.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a rapid screening method for antagonizing Trichoderma living organisms. Background technique [0002] As an important biological control microorganism, Trichoderma has a good control effect on a variety of plant diseases and has been widely used in the field. However, in order to obtain excellent antagonistic Trichoderma, multi-step screening under in vitro and in vivo conditions is required. The traditional screening method mainly includes three steps: plate primary screening, pot plant re-screening and field test. Among them, the re-screening of potted plants is an important part of testing the biocontrol potential of Trichoderma strains, and the workload is relatively large and the test time is relatively long. If there are many candidate Trichoderma strains obtained in the preliminary screening, the re-screening of potted plants will consume more manp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 王强强陈捷窦恺李婷婷
Owner SHANGHAI JIAOTONG UNIV
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