Method for detecting carboxylic acid flavor ingredients in tobacco products, mainstream smoke and heated non-burning cigarettes

Abstract

The invention relates to a method for detecting carboxylic acid flavor ingredients in tobacco products, mainstream smoke and heated non-burning cigarettes. According to the method, by virtue of signalidentification of differential marked tobacco products, mainstream smoke and heated non-burning cigarette samples, the problems that the ionization efficiency is low and the sensitivity is insufficient during mass spectrometric detection of the carboxylic acid flavor ingredients are solved, and non-targeting analysis of the carboxylic acid flavor ingredients in the samples is realized.

Description

technical field

[0001] The invention relates to the technical field of tobacco chemical inspection, in particular to a method for detecting carboxylic acid aroma components in tobacco products, mainstream smoke or heat-not-burn cigarettes based on stable isotope labeling mass spectrometry technology. Background technique

[0002] The chemical composition of tobacco is closely related to its quality, which in turn affects the aroma and taste of smoke, so the study of aroma components has always been valued by people. With the sharpening of smoking and health problems and the development of cigarette tar reduction technology, the requirements for cigarette flavoring and additives are getting higher and higher, and higher requirements are also put forward for the research work of smoke aroma substances. Carboxylic acid aroma components are important aroma components in smoke. Currently, more than 275 carboxylic acid aroma components have been identified in smoke, and more than ...

Examples

Embodiment 1

[0055] The detection of carboxylic acid aroma components in a flue-cured tobacco leaf based on stable isotope labeling mass spectrometry technology includes the following steps:

[0056] (1) Preparation of tobacco leaf sample solution: add acetonitrile (15 mL per gram of sample) to flue-cured tobacco leaves for ultrasonic extraction for 30 min, and the extract is filtered through an organic phase filter and used as a sample solution for later use.

[0057] (2) Isotope labeling of the sample solution: take 200 μL of the sample solution in step (1), add 10 μL of 2 μmol / mL triethylamine and 10 μL of 120 μmol / mL 2-chloro-1-methylpyridinium iodide and mix well, Add 20 μL of 60 μmol / mL N,N-dimethylethylenediamine and shake at 50°C for 2 hours. After the reaction was completed, the reaction system was frozen at -20°C for 10 minutes to terminate the reaction, blown dry with slow nitrogen flow, and the obtained solid sample was redissolved in acetonitrile / water (50 / 50, v / v) to obtain "...

Embodiment 2

[0069] Adopt the method described in embodiment 1 to analyze certain Burley tobacco leaf, obtain as follows image 3 Total ion chromatograms shown for neutral losses at 45 and 49 Da. A total of 120 possible carboxylic acid fragrance components as shown in Table 2 were obtained through screening.

[0070] Table 2 Potential carboxylic acid aroma components in a Burley tobacco leaf

[0071]

[0072]

[0073]

Embodiment 3

[0075] Based on stable isotope labeling mass spectrometry, the analysis of carboxylic acid aroma components in the mainstream smoke of a certain flue-cured cigarette includes the following steps:

[0076] (1) Preparation of cigarette mainstream smoke sample solution: According to the conditions specified in GB / T 19609-2004 and ISO 4387:2002, the cigarettes were smoked with a linear smoking machine for 4 cigarettes each time, and the total amount of cigarette mainstream smoke captured was collected. The filter disc of the granular phase is placed in a container, and acetonitrile (15 mL per gram of sample) is added for ultrasonic extraction for 30 min. After the extract is filtered through an organic phase filter, it is used as a sample solution for later use.

[0077] (2) Isotope labeling of the sample solution: take 200 μL of the sample solution in step (1), add 10 μL of 2 μmol / mL triethylamine and 10 μL of 120 μmol / mL 2-chloro-1-methylpyridinium iodide and mix well, Add 20 μL...

technical field

[0001] The invention relates to the technical field of tobacco chemical inspection, in particular to a method for detecting carboxylic acid aroma components in tobacco products, mainstream smoke or heat-not-burn cigarettes based on stable isotope labeling mass spectrometry technology. Background technique

[0002] The chemical composition of tobacco is closely related to its quality, which in turn affects the aroma and taste of smoke, so the study of aroma components has always been valued by people. With the sharpening of smoking and health problems and the development of cigarette tar reduction technology, the requirements for cigarette flavoring and additives are getting higher and higher, and higher requirements are also put forward for the research work of smoke aroma substances. Carboxylic acid aroma components are important aroma components in smoke. Currently, more than 275 carboxylic acid aroma components have been identified in smoke, and more than ...

Examples

Embodiment 1

[0055] The detection of carboxylic acid aroma components in a flue-cured tobacco leaf based on stable isotope labeling mass spectrometry technology includes the following steps:

[0056] (1) Preparation of tobacco leaf sample solution: add acetonitrile (15 mL per gram of sample) to flue-cured tobacco leaves for ultrasonic extraction for 30 min, and the extract is filtered through an organic phase filter and used as a sample solution for later use.

[0057] (2) Isotope labeling of the sample solution: take 200 μL of the sample solution in step (1), add 10 μL of 2 μmol / mL triethylamine and 10 μL of 120 μmol / mL 2-chloro-1-methylpyridinium iodide and mix well, Add 20 μL of 60 μmol / mL N,N-dimethylethylenediamine and shake at 50°C for 2 hours. After the reaction was completed, the reaction system was frozen at -20°C for 10 minutes to terminate the reaction, blown dry with slow nitrogen flow, and the obtained solid sample was redissolved in acetonitrile / water (50 / 50, v / v) to obtain "...

Embodiment 2

[0069] Adopt the method described in embodiment 1 to analyze certain Burley tobacco leaf, obtain as follows image 3 Total ion chromatograms shown for neutral losses at 45 and 49 Da. A total of 120 possible carboxylic acid fragrance components as shown in Table 2 were obtained through screening.

[0070] Table 2 Potential carboxylic acid aroma components in a Burley tobacco leaf

[0071]

[0072]

[0073]

Embodiment 3

[0075] Based on stable isotope labeling mass spectrometry, the analysis of carboxylic acid aroma components in the mainstream smoke of a certain flue-cured cigarette includes the following steps:

[0076] (1) Preparation of cigarette mainstream smoke sample solution: According to the conditions specified in GB / T 19609-2004 and ISO 4387:2002, the cigarettes were smoked with a linear smoking machine for 4 cigarettes each time, and the total amount of cigarette mainstream smoke captured was collected. The filter disc of the granular phase is placed in a container, and acetonitrile (15 mL per gram of sample) is added for ultrasonic extraction for 30 min. After the extract is filtered through an organic phase filter, it is used as a sample solution for later use.

[0077] (2) Isotope labeling of the sample solution: take 200 μL of the sample solution in step (1), add 10 μL of 2 μmol / mL triethylamine and 10 μL of 120 μmol / mL 2-chloro-1-methylpyridinium iodide and mix well, Add 20 μL...

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