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Tissue culture method of saffron

A tissue culture, saffron technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of virus accumulation, affecting the quality of saffron, flower sterility, etc., and achieve easy callus induction. , Improve the survival rate of transplanting, overcome the effect of slow reproduction

Active Publication Date: 2020-10-09
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, saffron flowers are sterile and fail to produce viable seeds
The traditional propagation system, such as the slow propagation by dividing balls, leads to the accumulation of viruses and affects the quality of saffron. Therefore, it is expected that propagation through tissue culture will provide a potential and effective method

Method used

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  • Tissue culture method of saffron
  • Tissue culture method of saffron
  • Tissue culture method of saffron

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: a kind of tissue culture method of saffron, carry out following steps successively:

[0057] 1) Take saffron bulbs that grow robustly and are free from diseases and insect pests, break off the side buds (about 1-2 cm in length), soak the side buds in 1% washing powder aqueous solution for 30 minutes, and then put them into gauze bags Rinse with tap water for 1-2 hours;

[0058] 2), callus induction:

[0059] After the lateral buds after the above-mentioned flowing water washing are routinely disinfected (i.e. 0.1% w / v HgCl 2 After 6 to 8 minutes of treatment, rinse with sterile water 5 to 6 times, and then blot dry with sterile filter paper), place it horizontally on the callus induction medium for culture; until callus growing in clusters is formed; culture time About 60 days.

[0060] The culture conditions are: 16 hours of light, light intensity 30-40 μmol m -2 ·s -1 , the temperature is (25±1)°C; 8 hours of dark cultivation, the temperature is (21...

Embodiment 2

[0082] Compared with Example 1, the hormones in the medium used in steps 2) to 3) have been adjusted, namely:

[0083] Adventitious bud induction medium: MS+TDZ1mg / L+PIC2mg / L+white sugar 25g / L+agar 8g / L, pH 5.8.

[0084] Callus induction medium: MS+6-BA10mg / L+NAA 2mg / L+white sugar 25g / L+agar 8g / L, pH 5.8.

[0085] The rest are equal to Example 1.

[0086] The result is:

[0087] During the culture process of induced callus in step 2), the induction rate of embryogenic callus reaches 89%.

[0088] In the step 3) adventitious bud induction culture, the adventitious bud induction rate was 76%.

[0089] Compared with Example 1, Example 2 adjusted the hormone concentration in callus induction and adventitious bud induction.

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Abstract

The invention discloses a tissue culture method of saffron. The tissue culture method comprises the following steps: breaking off lateral buds of saffron bulbs to be washed, disinfected and cut; thenhorizontally placing and inoculating into a callus inducing culture medium for cultivating until forming cluster-grown calluses; dividing the calluses into small pieces and inoculating into an inducing adventitious bud culture medium for cultivating until growing adventitious buds; cutting the adventitious buds and inoculating into a bulb inducing culture medium to obtain small bulbs I; and inoculating the small bulbs I onto a strong seedling culture medium for cultivating strong seedlings to obtain small bulbs II capable of being discharged from a bottle to be planted and transplanted. The tissue culture method can obtain a large number of saffron tissue culture seedlings.

Description

technical field [0001] The invention relates to a tissue culture method of saffron, in particular to a tissue culture rapid propagation method of saffron. Background technique [0002] Saffron, because its stigma can be used as a spice and medicine to treat diseases, is widely cultivated, and can be widely used in pharmaceutical, food processing and coloring industries. However, saffron flowers are sterile and fail to produce viable seeds. The traditional propagation system, such as the slow propagation by dividing balls, leads to the accumulation of viruses and affects the quality of saffron. Therefore, it is expected that propagation through tissue culture will provide a potential and effective method. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a tissue culture method of saffron, by which a large amount of tissue cultured seedlings of saffron can be obtained. [0004] In order to solve the problems of th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 毛碧增马阳阳何伯伟
Owner ZHEJIANG UNIV
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