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Composition and culture medium containing the same

A culture medium and ph value technology, applied in the field of microbial cultivation, to achieve the effect of solving long-term storage and improving storage time

Active Publication Date: 2021-11-26
SHENZHEN BLOT BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This medium can greatly increase the storage time of Helicobacter pylori under different low temperature conditions, effectively solving the problem of long-term storage of Helicobacter pylori strains

Method used

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  • Composition and culture medium containing the same
  • Composition and culture medium containing the same
  • Composition and culture medium containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] formula:

[0034]

[0035] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.2, and sterilize under high pressure in a high-pressure steam sterilizer at 121°C for 20 minutes.

Embodiment 2

[0037] formula:

[0038]

[0039] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.3, and sterilize under high pressure in a high-pressure steam sterilizer at 121°C for 20 minutes.

Embodiment 3

[0041] formula:

[0042]

[0043] After mixing, heat to dissolve, then filter with filter paper, adjust the pH value to 7.1, and sterilize under high pressure in a high-pressure steam sterilizer at 121°C for 20 minutes.

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PUM

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Abstract

The invention relates to the field of microorganism cultivation, in particular to a composition and a culture medium containing the composition. The invention provides a composition and a preservation medium containing the composition including bovine heart extract, bovine brain extract, cysteine, Na 2 HPO 4 , peptone and NaCl. Through the proportion of composition, buffer system and cell protection agent, the storage time of Helicobacter pylori can be greatly improved under different low temperature conditions, and the problem of long-term storage of Helicobacter pylori strains can be effectively solved, especially suitable for Helicobacter pylori specialty The strain library requires long-term strain preservation.

Description

technical field [0001] The invention relates to the field of microorganism cultivation, in particular to a composition and a culture medium containing the composition. Background technique [0002] Helicobacter pylori or Helicobacter pylori, English name Helicobacter pylori, referred to as Hp. Gram-negative, microaerophilic bacteria that live in various regions of the stomach and duodenum. It can cause mild chronic inflammation of the gastric mucosa and even lead to gastric and duodenal ulcers and gastric cancer. Helicobacter pylori is a spiral-shaped, micro-anaerobic bacteria that requires very harsh growth conditions. In 1983, it was successfully isolated from the gastric mucosal biopsy tissue of patients with chronic active gastritis for the first time. It is the only microbial species known to be able to survive in the human stomach. Epidemiological analysis shows that the infection rate is as high as 50% to 70% worldwide. The detection rate of HP in chronic active g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/01
CPCC12N1/04C12N1/20
Inventor 王洪涛林骏刘尧马伟民张永顶刘谦
Owner SHENZHEN BLOT BIOTECH
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