Sonic sorting
A technology of sound wave and sound wave generator, applied in the field of sound wave sorting
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example 1
[0085] This example demonstrates the enhancement of surface acoustic wave cell sorting by utilizing vertical flow-focusing junctions and sloped ceiling grooves. These devices may be able to approach performance levels of state-of-the-art fluorescence-activated cell sorters in terms of event rate and purity. This example demonstrates a microfluidic cell sorter that sorts cells at rates approaching those of commercially available FACS. This device combines a three-dimensional flow-focusing nozzle with sloped ceiling grooves to enhance the capabilities of the SAW transducer by utilizing components of the SAW oriented perpendicular to the plane of the substrate. This example determines conditions for device performance and uses these principles to implement a cell sorter. The device achieves sorting at a speed of 9000 events / sec at a purity of 55% and a purity of 90% when operated at a rate of 1000 events / sec.
[0086] Without wishing to be bound by any theory, it is believed th...
example 2
[0093] In this example, the capability of an inclined channel device for cell sorting applications was tested by determining that applied SAW pulses were generally successful in redirecting target cells to conditions that preserved exit. As a basis for the test, a device made of poly(dimethylsiloxane) (PDMS) with a vertical flow focusing nozzle 50 microns wide and 25 microns high; a sorting channel 250 microns wide and 50 microns high was used and a rectangular groove 120 microns wide, rising 25 microns above the sorting channel (eg, which can hold the cells) and inclined 60° from the overall flow direction. These dimensions are not intended to be limiting. Whenever a cell of interest is detected (eg, due to fluorescence), the instrument triggers a SAW pulse and uses a high-speed camera to capture the trajectory of each cell. Each resulting movie is analyzed to extract the trajectory of the target cell; a given sorting event is considered successful if the desired cell exits ...
example 3
[0097] This example determines the minimum length of SAW pulse required for a diagonal channel device to reproducibly actuate cells into a sorting channel for a range of different RF power levels and cell types. As the radio frequency (RF) power used to generate the SAW increases, the shorter pulses provide enough energy to redirect the cells into the trench. Cells can be efficiently actuated with pulses as short as 20 microseconds. For model adherent and non-adherent cell types, cells were reproducibly deflected into grooves with similar SAW pulse parameters, as Figure 3A shown in . However, one cell line consistently required less energy to deflect than the other, suggesting that the two lines may have inherent differences in their average size or acoustic contrast. Nonetheless, the inclined channel device can actuate both adherent and non-adherent cells, and the parameter range for efficient actuation is compatible with high-speed sorting applications.
[0098] In this ...
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