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Dietary peptides

一种多肽、选自的技术，应用在肽、缩肽、激素肽等方向，能够解决回肠制动器确切机制不清楚等问题

Inactive Publication Date: 2018-08-31
EATING & LIVING PTE LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the exact mechanism behind the ileal brake is unknown

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0068] Preparation of the polypeptide of the present invention

[0069] The invention also relates to methods of preparing the polypeptides of the invention as described above. Methods of their synthesis or preparation include, but are not limited to, recombinant (whether produced from cDNA, genomic DNA, synthetic DNA or other forms of nucleic acid), synthetic and transgenic methods.

[0070] The polypeptides of the invention described herein can be produced by recombinant nucleic acid techniques. Typically, a nucleic acid sequence encoding a desired polypeptide is inserted into an expression vector, which is then transformed or transfected into a host cell.

[0071] Alternatively, and preferably, the polypeptides of the invention are produced by synthetic means, ie by polypeptide synthesis. In some embodiments, the present invention is directed to methods of making analogs of unnatural amino acids comprising a total of about 5 residues to a total of about 20 residues. In s...

Embodiment 1

[0148] Assay:

[0149] Ca 2+ Flow measurement:

[0150] Elevation of intracellular calcium levels was measured using the fluorescent calcium chelating dye Fluo-4AM (ThermoFischer Scientific, Denmark). Briefly, cells were grown to near confluence in monolayers in 96-well tissue culture plates (Sarstedt, Germany) in appropriate media as described in the cell culture section. Before the start of the assay, cells were incubated for 60 minutes at 37°C with 1.5 μM Fluo-4AM in complete medium containing 25 mM HEPES (pH 7.4), 1% BSA (Sigma-Aldrich, Denmark, 2 Hank's balanced salt solution (HBSS, ThermoFischer Scientific, Denmark) of % ink (Soluro GMBH, Germany), 0.01% poloxamer F-127 (Sigma-Aldrich, Denmark) and 1 mM probenecid (Probenecide) (Sigma-Aldrich) ) to mix 1:1.

[0151] All test compounds were dissolved in water and then diluted in Ix HBSS containing 25 mM HEPES (pH 7.4), 1% BSA and 2% ink. Test compounds were added directly to the wells without removing excess Fluo-4AM...

Embodiment 2

[0168] 1) Structure-activity relationship and stability (SAR)

[0169] a. Extended version

[0170] b. Alternative version

[0171] 2) In vivo studies in mice

[0172] c. Acute effects on feed intake (satiety)

[0173] d. Measurable long-term effects on body weight

[0174] Based on structural modeling studies of the interaction of DC7-2 and NTR-1, peptides of octapeptide, heptapeptide, hexapeptide, or pentapeptide can be predicted to exhibit increased potency due to increased binding.

[0175] Peptides with increased potency and stability can be predicted and observed compared to SAR studies using synthetic peptides.

[0176] Determination:

[0177] Synthetic peptides:

[0178] Based on the sequence of the natural hormone neurotensin (QLYENKPRRPYIL), the bioactive neurotensin fragment NT(8-13) (RRPYIL) and the identified bioactive octapeptide DC7-2 were synthesized using standard techniques (Schafer-N, Denmark) (ASDKPYIL), a synthetic peptide with systematic substituti...

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PUM

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Abstract

The present invention relates to novel peptides, composition comprising such peptides including nutritional supplements and methods for inducing satiation and satiety, for weight management and preventing or reducing the incidence of obesity, or for preventing or reducing cardiovascular diseases, atherosclerosis, hypertension, hepatosteatosis, cancer and / or diabetes.

Description

field of invention [0001] The present invention relates to novel peptides, compositions comprising such peptides, including nutritional supplements, and for inducing satiety and satiety, preventing metabolic syndrome (including overweight and obesity, cardiovascular disease, atherosclerosis, hypertension, fatty liver (hepatosteatosis), diabetes and / or cancer) or a method of reducing the incidence thereof. Background of the invention [0002] Obesity is a common medical condition that affects many people worldwide and is associated with, induces or increases the risk of developing said conditions such as cardiovascular disease, atherosclerosis, hypertension, fatty liver, cancer and / or diabetes . [0003] Several regulators of obesity have been identified. However, despite intensive research, the regulation of obesity remains poorly understood. [0004] Protein is more satiating than carbohydrates and fats, and has an effect on food intake beyond its energy content alone. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/04C07K7/08C07K14/00
CPCA61K38/00C07K14/57509C07K7/06A61K38/04A61P1/16A61P3/04A61P35/00A61P9/10A61P9/12A61P3/10C07K14/4716A23L33/18A23V2002/00A23V2200/332A23L33/17A23L29/03C07K7/08
Inventor J·施特格斯戴德周婕慧R·耶森J·帕姆菲尔德特E·T·汉森
Owner EATING & LIVING PTE LTD
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