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Sperm optimization device and sperm optimization method used for horizontal intracytoplasmic single sperm injection

A single-sperm, horizontal technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as poor quality sperm, to protect rights and interests, improve clinical pregnancy rate, improve The effect of patient satisfaction

Inactive Publication Date: 2018-09-07
LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of gradient centrifugation or direct centrifugation is to separate the sperm from the semen, remove impurities, and separate the viable sperm from the inactive sperm, but the viable sperm obtained by the above method will also be There are poor quality sperm, which is the disadvantage of gradient centrifugation or direct centrifugation, so further optimization of sperm is required

Method used

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  • Sperm optimization device and sperm optimization method used for horizontal intracytoplasmic single sperm injection
  • Sperm optimization device and sperm optimization method used for horizontal intracytoplasmic single sperm injection
  • Sperm optimization device and sperm optimization method used for horizontal intracytoplasmic single sperm injection

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: A kind of sperm optimization device for horizontal intracytoplasmic sperm injection, such as Figure 1-Figure 2 As shown, it includes a preferred cell 1 for sperm. The preferred cell 1 for sperm is separated by a partition plate 2 to form a sperm storage pool 11 and a high-quality sperm pool 12. The partition plate 2 is provided with a sperm swimming channel 21. The sperm swimming channel is connected to the sperm storage pool and the high-quality sperm pool. A constant-temperature heating plate I3 for heating the sperm storage pool is arranged under the sperm storage pool 11, and a constant-temperature heating plate II4 for heating the high-quality sperm pool is arranged under the high-quality sperm pool 12. The constant temperature heating plate I and the constant temperature heating plate II are respectively fixed on the lower end surface of the sperm preferred tank body 1 .

[0020] In this embodiment, the sperm swimming channel has a length of 8-30 mm...

Embodiment 2

[0023] Embodiment 2: A method for optimizing sperm for horizontal intracytoplasmic sperm injection, using the sperm optimizing device described in Example 1, comprising the following steps:

[0024] ⑴ Add semen to the sperm storage pool and high-quality sperm pool, and then turn on the constant temperature heating plate Ⅰ and constant temperature heating plate Ⅱ;

[0025] (2) Control the temperature of the liquid in the sperm storage pool to 35°C, and the liquid temperature in the high-quality sperm pool to 38°C. Put the sperm after gradient centrifugation or direct centrifugation into the sperm storage pool, and the sperm with good vitality will swim through the sperm swimming channel. For the high-quality sperm pool, the timing starts from the time when the sperm are put into the sperm storage pool. After 10-20 minutes, the sperm in the high-quality sperm pool are taken as the sperm with good vitality.

[0026]

[0027] Note: The motility of sperm is divided into 4 grades...

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Abstract

The invention relates to a sperm optimization device and a sperm optimization method used for horizontal intracytoplasmic single sperm injection. The sperm optimization device comprises a sperm optimization trough, the interior of the sperm optimization trough is divided into a sperm storage pool and a high-quality sperm pool by a clapboard, a sperm swimming channel is formed in the clapboard, thesperm swimming channel is communicated with the sperm storage pool and the high-quality sperm pool, and a constant temperature heating plate I and a constant temperature heating plate II are respectively arranged below the sperm storage pool and the high-quality sperm pool; according to the sperm optimization method, sperm with good activity swims from the sperm storage pool with low temperatureto the high-quality sperm pool with high temperature through the sperm swimming channel by utilizing the temperature difference between the sperm storage pool and the high-quality sperm pool. According to the invention, sperm obtain by a gradient centrifugation method can be further optimized, sperm with relatively good activity is selected, and the selected sperm is used for fertilization, so asto improve the fertilization rate of ICSI and the development ability of embryos after ICSI, and finally increase the clinical pregnancy rate.

Description

technical field [0001] The invention relates to a sperm selection device and method, in particular to a sperm selection device and sperm selection method for horizontal intracytoplasmic sperm injection. Background technique [0002] Intracytoplasmic Sperm Injection (ICSI), also known as the second generation of "test-tube baby", uses a microscopic operating system to inject a single sperm into an egg to fertilize it. [0003] Since ICSI is mainly aimed at patients with poor sperm quality or low quantity, the sperm of these patients often have various defects, such as poor top-bottom reaction, low quantity, poor motility, low density, etc., so ICSI operation is required . During the ICSI operation, the motile sperm is aspirated with the ICSI needle under the microscope, and then the sperm is injected into the cytoplasm of the oocyte to complete the fertilization process. The sperm quality cannot be screened during the ICSI operation, only the sperm that can be seen under th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00C12N5/076
CPCC12M47/04C12M23/48C12M41/18C12M45/05C12N5/061
Inventor 李楠林忠李忻琳韦立红王麟李红辉仇雪嫦
Owner LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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