Quantum dot immunochromatographic detection card and quantum dot immunochromatographic detection method for detecting oxaprozin by using double-antibody sandwich immune competition method
A double-antibody sandwich and oxaprozine technology, which is applied in measurement devices, analytical materials, material excitation analysis, etc., can solve the problems of insufficient stability, low detection signal strength and sensitivity, and inability to uniformly prepare signal substances, and achieves active Favorable protective effect, short detection time, and the effect of increasing antibody stability
Active Publication Date: 2018-09-07
SHAOGUAN COLLEGE
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Problems solved by technology
The "binary system immunocompetition method" has the following disadvantages: 1. It is necessary to label the signal substance for each detection antibody separately, so that the signal substance cannot be prepared uniformly; 2. The detection antibody is in the liquid phase and has insufficient stability; 3. The detection signal intensity and less sensitive
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[0029] see figure 1 , which is the quantum dot immunochromatography detection card for detecting oxaprozine by the double-antibody sandwich immunocompetitive method of this embodiment, including a PVC liner, and sequentially adhered to the PVC liner and partially overlapped between adjacent ones. Detect antigen release pad, quantum dot probe release pad, nitrocellulose membrane (NC membrane) and water-absorbing pad; Described detection antigen release pad comprises the detection antigen that is formed by coupling of OVA and oxaprozine; Said quantum dot The probe release pad contains quantum dot probes labeled with anti-OVA antibodies; the nitrocellulose membrane is provided with detection lines and quality control lines, the detection lines are immobilized with anti-oxaprozin antibodies, and the quality control lines are immobilized with OVA antigen.
[0030] Specifically, the preparation method of the detection antigen release pad is as follows: a glass cellulose membrane wi...
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The invention relates to a quantum dot immunochromatographic detection card and a quantum dot immunochromatographic detection method for detecting oxaprozin by using a double-antibody sandwich immunecompetition method. The detection card comprises a lining plate, a detection antigen release pad, a quantum dot probe release pad, a chromatographic membrane and an absorbent pad, wherein the detection antigen release pad contains a detection antigen formed by coupling ovalbumin and oxaprozin; the quantum dot probe release pad contains a quantum dot probe labeled with an anti-ovalbumin antibody; the chromatographic membrane is provided with a detection line and a quality control line, wherein the detection line immobilizes an oxaprozin antibody, and the quality control line immobilizes the ovalbumin antigen. The detection antigen is dissolved into a detection system from the release pad, and a double-antibody sandwich immune complex is formed by the detection antigen together with a detection antibody and a labeled antibody, so that a fluorescence detection signal is generated. Under the detection and reference function of the quality control line, whether a sample contains the oxaprozin or not can be judged according to the fluorescence signal on the detection line, so that the signal intensity and stability of the detection can be improved, and the detection sensitivity and the quantitative precision are enhanced.
Description
technical field [0001] The invention relates to the field of food and drug safety detection, in particular to a method of applying immunochemical detection technology to the detection of illegally added chemicals in health products and Chinese patent medicines, especially the quantum dot immune layer of oxaprozine detected by the double-antibody sandwich immunocompetition method Analysis test card and test method. Background technique [0002] The chemical name of oxaprozine is 2-(-4-isobutylphenyl)propionic acid, which belongs to non-steroidal anti-inflammatory drugs, has anti-inflammatory, analgesic and antipyretic effects, and is used for rheumatoid arthritis, adhesion spondylitis, non-inflammatory joint pain, arthritis, non-articular rheumatism, pain caused by non-articular inflammation, various neuralgia, cancer pain, post-traumatic pain and fever caused by various inflammations, etc., but there are certain It is a prescription drug clinically and must be taken under t...
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Login to View More IPC IPC(8): G01N21/64G01N33/558G01N33/533
CPCG01N21/6428G01N21/6486G01N33/533G01N33/558
Inventor郭杰标林秀玉谢伟光蒋昊黄钻元朱哲吴蓝洁张鹏
OwnerSHAOGUAN COLLEGE