Field herbaceous plant environment control test method

A herbaceous plant and control experiment technology, which is applied in the field of plant physiological and ecological research, can solve the problems of death, missing, and the inability to further explain the changes of plant mechanisms, so as to avoid cross-contamination and ensure accuracy.

Inactive Publication Date: 2018-09-11
新疆维吾尔自治区环境保护科学研究院
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Problems solved by technology

[0008] Field observation and laboratory cultivation methods are mostly used. It can be seen that the condition of "field observation" is the original growth environment of plants, and the experimental results are the most realistic display under natural conditions. However, the biggest disadvantage is that the gradient changes of environmental factors are difficult to control. , unable to further explain the changes in plant mechanisms
The single-factor or two-factor environmental gradient in "laboratory cultivation" is easier to control, but the soil environment is not the original natural condition, and the influence of groundwater on plant growth is ignored, some important results may be missed, and the changing soil environment may cause Salt and drought tolerant plants grow poorly or even die later

Method used

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  • Field herbaceous plant environment control test method
  • Field herbaceous plant environment control test method
  • Field herbaceous plant environment control test method

Examples

Experimental program
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Embodiment 1

[0035] (1) Selection of sample plots for planting: in the desert areas where the native distribution of Huachai is selected, the area with flat terrain and little difference in soil background value is selected (24×14=336m 2 ), as a salt gradient test site;

[0036] Three gradients (mild salinization, moderate salinization, and severe salinization, set according to Table 1) were adopted. Select 6 plots of 6m×6m (Huahuachai is a perennial herb), with a buffer zone of at least 2m between each plot, one of which is a reserved test area, and each of the other 5 plots It is further divided into 4 plots, namely "control area", "mild salinization area", "moderate salinization area" and "severe salinization area", that is, there are 20 plots in total. Partitions (depth 1m) are placed around the plot to block the lateral exchange of salt and water, and to prevent the generation of cloned plants from affecting the experimental results. The sample setting is as follows: figure 1 , im...

Embodiment 2

[0050] (1) Selection of sample plots for planting: In desert areas where camel thorns are originally distributed, select areas with flat terrain and little difference in soil background values ​​(34*22=748m 2 ), as the water gradient test site; for the isosmotic potential test corresponding to the salt gradient (recorded as PEG-light, PEG-medium, PEG-heavy), the reagent is PEG solution, and six 10m×10m (camel thorns are selected as semi-shrub), the other methods are the same as the salt gradient group, and the sample setting is as follows figure 2 , Figure 4 , Figure 6 .

[0051] (2) the plant selection of planting: dry environment plant selects camel thorn, selects robust 2-3 year old seedling stock, seedling height 5-7cm, root length 20-30cm, plant spacing 1.5m, pit diameter 60cm, depth of pit 70cm, will Xerophytes were planted in the pit, and the seedlings were backfilled with soil to a distance of 10 cm from the pit mouth.

[0052] (3) In the first ten days of April...

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Abstract

The invention discloses a field herbaceous plant environment control test method. The method comprises the following steps that 1, a planting sample plot is selected; 2, plants to be planted are selected; 3, halophyte and xerophyte seedlings are planted in the sample plot in the first ten-day period of April of the first year, irrigation is conducted after the halophyte and xerophyte seedlings areplanted, and it is ensured that the water content of soil after irrigation reaches the saturation water content; 4, irrigation is carried out once after planting at the end of April, and it is ensured that seedling planting is successful through manual management; 5, salt and drought gradient test processing is completed in early May, and then field observation test and plant and soil sample collection are carried out according to requirements of research purposes in growth seasons including May, July and September; 6, in the middle ten days of April of the second growth year, irrigation is conducted once, and it is ensured that the saturation water content of the soil is reached after each time of irrigation. The method is low in cost, the salt and water gradient is obvious, control samples are easy to select, and the effect is good.

Description

technical field [0001] The invention belongs to the technical field of plant physiology and ecology research, and relates to a method for field herb plant environment control test. Background technique [0002] The relationship between organisms and the environment and their interactions have been continuously improved through natural selection during the long process of biological succession. During this process, the environment is constantly changing to affect the organisms, and the organisms are constantly evolving to adapt to and in turn affect the environment. Each species adapts to its physical and biological environment through certain physiological and ecological strategies, which can be manifested in biochemistry, physiology, morphology, behavior and group sociology, etc. Taking arid regions as an example, the continuous climate change characterized by changes in precipitation patterns and frequent extreme droughts affects the living environment of plants. grow. ...

Claims

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Application Information

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IPC IPC(8): A01G22/40A01G22/00
CPCA01G22/00A01G22/40
Inventor 秦璐阴俊齐邓葵杨林红伍德彦祖力克尔江·阿布都热西提阿孜古丽·阿不都拉何静
Owner 新疆维吾尔自治区环境保护科学研究院
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