Preparation method for extract of total flavonoids in kernel of Prinsepia utilis

A technology of green thorn nuts and total flavonoids, applied in the field of extraction, can solve the problems of application limitations, inconvenient recycling of macroporous resins, time-consuming and laborious separation and purification, etc., and achieve the effect of shortening the time

Active Publication Date: 2018-09-21
YUNNAN BOTANEE BIO TECH GRP CO LTD +1
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Problems solved by technology

[0006] At present, the extraction of the total flavonoids in the green thorn nuts is directly extracted with ethanol or methanol to obtain a crude extract, and then separated and purified to obtain the green thorn nut extract. There is no degreasing process for the preparation of high total flavonoids According to the research report of the green thorn nut extract, the total flavonoid content in the green thorn nut extract is low, ranging from 0.04 to 8wt.%, and the green thorn nut contains very high oil, generally accounting for 30wt. .%, which brings the following technical problems: the first, the oil will reduce the adsorption efficiency of the macroporous resin during purification, prolong the column chromatography time, and the macroporous resin column cannot be reused due to the adsorption of oil; the second, There is a relatively high content of oil in the resulting product, which is

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  • Preparation method for extract of total flavonoids in kernel of Prinsepia utilis
  • Preparation method for extract of total flavonoids in kernel of Prinsepia utilis
  • Preparation method for extract of total flavonoids in kernel of Prinsepia utilis

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preparation example Construction

[0032] The preparation method of the total flavonoids extract of green thorn nuts of the present invention comprises the following steps: a degreasing step, a reflux extraction step and a separation and purification step.

[0033] Degreasing steps:

[0034] In the preparation method of the total flavonoids extract of green thorn nuts of the present invention, a degreasing step is added before the reflux extraction step, so as to shorten the time of the separation and purification step, increase the content of total flavonoids in the extract and improve the efficiency. The degreasing step is based on the total oil yield as the final measure of the degreasing effect, and the best process conditions are selected.

[0035] According to the weight ratio of material to liquid of 1:3 to 1:8, the dried and crushed green thorn nuts are soaked in the degreasing agent, and the degreasing is carried out at room temperature for 1 to 3 times, and soaking is carried out during each degreasin...

Embodiment 1

[0048] Take 200 g of dried green thorn nuts, crush them, and degrease them with 600 mL of n-hexane for 3 times. During each degreasing process, soak and stir for 3 hours each time to obtain green thorn nut materials. Afterwards, the green thorn nut material was added into an ethanol aqueous solution with a volume concentration of 70vol.% at a ratio of 1:8 by weight ratio of solid to liquid, and refluxed and extracted twice at 75°C, and the two extracts were combined and enriched by filtration , then remove the ethanol in the extract by concentrating under reduced pressure to obtain a concentrated solution, then evaporate the concentrated solution to dryness in a water bath to obtain 16.23 g of crude extract, and take rutin as a standard contrast, and the total flavonoid content in this crude extract is 20.99wt .%.

Embodiment 2

[0050] Take 200 g of dried green thorn nuts, crush them, and degrease them with 600 mL of petroleum ether for 3 times. During each degreasing process, soak and stir for 3 hours each time to obtain green thorn nut materials. Afterwards, the green thorn nut material was added into an ethanol aqueous solution with a volume concentration of 70vol.% at a ratio of 1:8 by weight ratio of solid to liquid, and extracted twice under reflux at 75°C, and the two extracts were combined and enriched by filtration. Then, the ethanol in the extract was removed by concentration under reduced pressure to obtain a concentrated solution, and then the concentrated solution was evaporated to dryness in a water bath to obtain 15.87 g of crude extract. Taking rutin as a standard contrast, the total flavonoid content in this crude extract was 18.79wt. %.

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Abstract

The invention discloses a preparation method for the extract of total flavonoids in the kernel of Prinsepia utilis. The preparation method comprises the following steps: degreasing: removing grease indried and crushed kernels of Prinsepia utilis by using a degreasing agent, and evaporating the degreasing agent so as to obtain a Prinsepia utilis kernel material; reflux extraction: subjecting the Prinsepia utilis kernel material to reflux extraction by using an aqueous methanol solution with a volume concentration of 40 to 95 vol% or an aqueous methanol solution n with a volume concentration of40 to 95 vol% so as to obtain liquid extract, and drying the liquid extract by evaporation so as to obtain crude extract; and separation and purification: separating and purifying the crude extract by using a macroporous resin column and carrying out enrichment so as to obtain the extract containing the total flavonoids. The preparation method has the technical effects that the content of the total flavonoids in the extract is allowed to reach 30 wt% or more; and time for separation and purification is substantially shortened in the process of preparation.

Description

technical field [0001] The invention relates to a preparation method of total flavonoids extract of green thorn nuts in the technical field of extraction. Background technique [0002] Flavonoids (Flavonoids Compounds) are plant secondary metabolites, which widely exist in natural plants in the form of free or sugar-bound glycosides. Flavonoids exhibit a variety of pharmacological activities, can prevent and treat diseases of the cardiovascular and cerebrovascular systems and respiratory systems, have anti-inflammatory, antibacterial, hypoglycemic, anti-oxidation, anti-radiation, anti-cancer, anti-tumor and enhance immunity, etc. pharmacological effects. In recent years, the study of flavonoids has entered a new level. With the in-depth research on the relationship between their structures and functions, some mechanisms of action of flavonoids have been discovered, which provides a basis for their application in the fields of skin care products, medicine, and food. The the...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61P3/10A61P3/06
CPCA61K36/73A61K2236/333A61K2236/55A61P3/06A61P3/10
Inventor 曲丽萍吴信浪袁永雷王飞飞马骁郭振宇何黎
Owner YUNNAN BOTANEE BIO TECH GRP CO LTD
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