Oriental cherry breeding seedling method

A technology of cherry blossoms and sub-generation proliferation, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of lack of systematic research on garden application value, restricting the development of cherry blossoms, etc., to achieve high survival rate, reduce costs, and achieve stability. genetic effects

Inactive Publication Date: 2018-09-28
金华市野泉园艺技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many cherry blossoms planted in China, most of them are imported from Japan, which are not as adaptable as native tree species. There is a lack of systemati

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A kind of cherry blossom breeding seedling raising method, comprises the following steps:

[0017] 1) Pretreatment: Collect new semi-lignified cherry blossom branches, remove leaves with 3mm of petiole and the young part of the top of the branches, the cut length of cherry blossom branches is 1.5cm, and contains 1 axillary bud; shake with concentrated detergent solution Wash for 3 minutes, then rinse off the detergent residue with tap water, soak in 75% alcohol for 10 seconds in an ultra-clean workbench, soak in 0.08% HgCl solution for 5 minutes, rinse with sterile water 3 times, each time 3 times Minutes; Branch sterilization can kill harmful microorganisms carried by cherry blossom explants, avoid introducing harmful microorganisms into the culture medium to cause pollution, and at the same time avoid the absorption and utilization of microorganisms on the culture medium, and provide sufficient nutrients for the explant cells for its differentiation;

[0018] 2) Prim...

Embodiment 2

[0022] A kind of cherry blossom breeding seedling raising method, comprises the following steps:

[0023]1) Collect new semi-lignified cherry blossom branches, remove leaves with 5mm of petiole and the tender part of the top of the branches, the cut length of cherry blossom branches is 3.0cm, and contains 2 axillary buds; shake and wash with concentrated detergent solution for 5 minutes , then rinse off the detergent residue with tap water, soak in 78% alcohol for 15 seconds in an ultra-clean workbench, soak in 0.1% HgCl solution for 8 minutes, rinse with sterile water 5 times, 5 minutes each time; 2 ) to prepare the first-generation induction medium: agar 1.5g / L, carrageenan 6g / L, sugar 30g / L, folic acid 3.0mg / L, naphthaleneacetic acid 4.3mg / L, pH 6.2, glass rods inserted into 4cm deep small Insert a piece of cherry blossom branch vertically into each small hole, evenly spread 3mm thick powdered activated carbon, and cultivate it for 25 days under the culture conditions of te...

Embodiment 3

[0025] A method for propagating and raising seedlings of cherry blossoms, comprising: pretreatment, primary culture, subculture multiplication culture, and rooting culture, specifically comprising the following steps:

[0026] Pretreatment: collect new semi-lignified cherry blossom branches, remove leaves with 4 mm of petiole and tender part of the top of the branches, the cut length of cherry blossom branches is 2.5 cm, and contains 1 axillary bud; shake and wash with concentrated detergent solution for 3 minutes, then rinse off the detergent residue with tap water, soak in 75-78% alcohol for 10 seconds in the ultra-clean workbench, soak in 0.1% HgCl solution for 5 minutes, rinse with sterile water 5 times, each time 3 times Minutes; Branch sterilization can kill harmful microorganisms carried by cherry blossom explants, avoid introducing harmful microorganisms into the culture medium to cause pollution, and at the same time avoid the absorption and utilization of microorganis...

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PUM

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Abstract

The invention discloses an oriental cherry breeding seedling method. The oriental cherry breeding seedling method comprises following steps: pretreatment, initial culture, subculture multiplication culture, and rooting culture. According to the oriental cherry breeding seedling method, oriental cherry twigs are washed and sterilized for further processing; the oriental cherry twigs are introducedinto an initial culture medium, and active carbon powder is scattered for initial culture; multiple shoots obtained through initial culture are cut into bud segments, and then subculture multiplication culture is carried out; rooting culture and hardening seedling are carried out; and at last transplanting can be carried out. The beneficial effects are that: the oriental cherry breeding seedling method is simple and convenient, is capable of realizing stable inheritance of oriental cherry genetic materials, obtaining a large amount of oriental cherry seedlings from a small amount of oriental cherry buds, reducing cost greatly, and increasing oriental cherry multiple shoot differentiation rate and survival rate.

Description

technical field [0001] The invention relates to the field of cherry blossom breeding, in particular to a method for breeding cherry blossom seedlings. technical background [0002] Cherry blossoms( Prunus serrulata ) is a world-renowned ornamental flower and tree, belonging to the genus Prunus in the family Rosaceae, distributed in the warm regions of the northern hemisphere, and is a plant in the northern temperate zone. It has been recorded in Asia, Europe and North America. The genus includes more than 100 species of members, the main species are distributed in the west and southwest of my country, as well as Japan and Korea. Cherry blossoms are ornamental flowers and trees in early spring. They have beautiful plant shape, bright colors and long flowering period. They have great application value in gardens and can be widely used in parks, schools, streets, courtyards and other green spaces. Due to the high ornamental value of cherry blossoms, the introduction and culti...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王志刚
Owner 金华市野泉园艺技术有限公司
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