Method for rapidly inducing clustered buds by cultivating bretschneidera sinensis embryos

A technology of Bole tree and germ, which is applied in the field of rapid induction of clustered buds of Bole tree germination, can solve the problems of unsatisfactory rapid propagation of Bole tree, low multiplication coefficient, and low germination rate, so as to promote rapid germination, simplify the cumbersome process, and improve active effect

Active Publication Date: 2018-09-28
CHINA THREE GORGES CORPORATION
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AI Technical Summary

Problems solved by technology

At present, in China, the process of inducing the low germination rate of Bole tree seeds and the induction of clustered buds by embryos is not only complicated and the cycle is long, but also the multiplication coefficient is too low to meet the purpose of rapid propagation of Bole tree

Method used

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  • Method for rapidly inducing clustered buds by cultivating bretschneidera sinensis embryos
  • Method for rapidly inducing clustered buds by cultivating bretschneidera sinensis embryos
  • Method for rapidly inducing clustered buds by cultivating bretschneidera sinensis embryos

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Embodiment 1

[0022] The material of the present invention is derived from the bole tree seeds that were harvested in that year in Wufeng County, Hubei.

[0023] Bole tree seed treatment first pours the Bole tree seeds into plastic pots, and separates the red fleshy outer testa from the seeds by repeated rubbing, then filters the outer testa through running water, and then uses water selection to remove unfulfilled seeds, and The selected seeds are spread out with newspapers and dried in the shade, keeping the water content of the seeds between 35%-50%, and finally peel off the inner seed coat with branch scissors, and soak in gibberellin GA 3 (0.0, 2.0, 4.0) mg / L concentration in aqueous solution for 1-2-3h before use (see Table 1 for treatment methods).

[0024] Table 1 Bole tree seeds in different gibberellin GA 3 Germination rate and proliferation coefficient under different concentration and dark culture time (statistical results of culture 45d)

[0025]

[0026] Germ induction cu...

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Abstract

The invention relates to a method for rapidly inducing clustered buds by cultivating bretschneidera sinensis embryos. The method comprises the steps: washing red fleshy exopleura of bretschneidera sinensis seeds in flowing water, drying the bretschneidera sinensis seeds in a shady, cool and ventilated place, and keeping the water content of the seeds between 35% and 50%; then, removing white thinand crisp endotesta, and soaking the seeds in an aqueous solution with gibberellin GA3 concentration being 2.0mg / L for 2h; next, carrying out flowing water flushing as well as sterilization and disinfection treatment; and finally, inoculating the seeds into a culture medium N6+6-BA1.5mg / L+GA31.5mg / L+saccharose 30g / L+agar6g / L (PH5.8-6.0) on a superclean workbench to carry out dark culture for 30d so as to achieve the optimal effects that the germination rate of the bretschneidera sinensis seeds reaches 100% and the multiplication coefficient is 6.17.

Description

technical field [0001] The invention provides a rapid propagation method of Bole tree, in particular to a method for rapidly inducing clustered buds of Bole tree germ cultivation. Background technique [0002] Bole tree is a unique species in China and a national first-class protected tree species. It is known as "dragon and phoenix among plants", also known as Zhongcalyx. It is a single family of plants distributed in my country. As a relic species of the Tertiary paleotropical flora, it has important scientific value in the study of angiosperm phylogeny, paleogeography and paleoclimate. This species is scattered in the mountainous areas of the provinces south of the Yangtze River. Due to long-term serious damage, the existing mother tree resources are scarce, the seed setting rate is low, and natural regeneration is difficult. It is in a rare and endangered situation. It has been listed as a national first-class precious tree species and It is a national level key protect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马晓波张国禹黄桂云陈会员邱利文胡梅香吴笛汪磊张海波张定军吴锦华杨兰芳望雄英张俊李翩翩
Owner CHINA THREE GORGES CORPORATION
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