Special culture medium for quickly inducing bulb of bletilla striata

A culture medium and germination medium technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of time-consuming, labor-intensive cultivation methods, etc., to promote rapid growth, improve transplanting survival rate, improve The effect of pseudobulb differentiation rate

Active Publication Date: 2017-10-10
重庆市药物种植研究所
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AI Technical Summary

Problems solved by technology

Each of its steps requires a specific medium, and its culture method has defects such as labor-consuming and time-consuming as mentioned above

Method used

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  • Special culture medium for quickly inducing bulb of bletilla striata
  • Special culture medium for quickly inducing bulb of bletilla striata
  • Special culture medium for quickly inducing bulb of bletilla striata

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1 Rapid Induction Tissue Culture Method for Radix Bletachidae and Bulb

[0051] Step 1: For pod requirements, light brown and mature pods should be used. Regularly stored pods are very easy to be infected with bacteria and rupture, which cannot meet the annual production needs of white and tissue-cultured seedlings. Wrap them in kraft paper and store them in the refrigerator. It is stored at 4°C;

[0052] Step 2: Wash the surface of the uncracked fruit pods with 70% alcohol for 1-3 minutes, then soak the fruit pods in 0.1% mercury chloride on the ultra-clean workbench for 15-20 minutes, and then use Rinse with sterile water for 3-5 times, dry the surface moisture with sterile filter paper for inoculation; cut the sterilized fruit pods with sterile scissors on the ultra-clean workbench, and dip the seeds with a sterile inoculation loop Apply evenly on the high temperature sterilized medium, the medium formula is 1 / 2MS+NAA0.1mg / L+GA 3 0.1mg / L+agar powder 6.5-7.0...

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Abstract

The invention discloses a special culture medium for quickly inducing bulb of bletilla striata. The special culture medium consists of a seed of bletilla striata germinating culture medium and a bulb high-efficiency inducing and seedling sounding culture medium, wherein the seed of bletilla striata germinating culture medium is prepared from the following components according to a formula: 1/2MS, 0.1mg/L of NAA (naphthylacetic acid), 0.1mg/L of GA3, 6.5 to 7.0g/L of agar, and 20g/L of white sugar, and the pH (potential of hydrogen) value is 5.8 to 6.0; the bulb high-efficiency inducing and seedling sounding culture medium is prepared from the following components according to a formula: 1/2MS, 0.5 to 0.6mg/L of NAA, 0.6 to 0.8mg/L of 6-BA, 0.4 to 0.5mg/L of PP333, 30 to 45g/L of white sugar, 25 to 40g/L of mashed banana, and 0.1g/L of activated carbon, and the pH (potential of hydrogen) value is 5.8 to 6.0. The invention also discloses a tissue culture method of the special culture medium. The tissue culture method has the advantages that the production steps of the tissue culture seedling of the bulb of bletilla striata are simplified, the inducing of pseudobulb, quick growth of pseudobulb and rooting and seedling sounding culture are completed at one time, the period from seeds to transplanting is 90 days, the culture time is shortened, the differentiation rate and quick growth of the pseudobulb are promoted, and the transplanting success rate of the tissue culture seedling is increased.

Description

technical field [0001] The invention belongs to the technical field of bletilla bulbs, and in particular relates to a special culture medium and a tissue culture method for rapidly inducing bletilla bulbs. Background technique [0002] Bletilla striata (Thunb.) Reichb.f. is a plant of the orchid family Bletilla striata (Thunb.) Reichb.f., and its pseudobulb is a medicinal part, which has the effects of astringing and stopping bleeding, reducing swelling and promoting granulation. Now research has found that Bletilla striata is effective in treating tuberculosis, stomach disease, The effect on tumors and other aspects is obvious, and it is also a high-quality chemical material. However, the white and white plants have been excavated and destroyed by the mirror, and the wild resources are very scarce. It has been listed as a second-class protected species of rare plants. [0003] With the development needs of white and its development and utilization value, its market demand i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘燕琴刘春雷刘杰刘旭胡开志李娜田婷
Owner 重庆市药物种植研究所
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