Beta-thalassemia gene amplification kit and method based on recombinase polymerase amplification technology

Abstract

The invention discloses a beta-thalassemia gene amplification kit and a method based on a recombinase polymerase amplification technology. The kit comprises RPA amplification primers which are respectively P1F-1 shown as SEQ ID NO. 1, P1Rbio-1 shown as SEQ ID NO. 2, P2F-1 shown as SEQ ID NO. 3, P2Rbio-1 shown as SEQ ID NO. 4, P3F-1 shown as SEQ ID NO. 5 and P3Rbio-1 shown as SEQ ID NO. 6. The amplification is performed on genomic DNA of a to-be-detected specimen by using the RPA amplification primers and can be completed within 30 minutes, and the sensitivity is high; an obtained amplificationproduct can be detected by a liquid phase chip, and the accuracy rate is high.

Description

technical field [0001] The invention relates to a beta-thalassemia gene amplification kit and method, in particular to a beta-thalassemia gene amplification kit and method based on recombinase polymerase amplification technology. Background technique [0002] Thalassemia, referred to as thalassemia, is a genetic hemolytic disease caused by globin synthesis disorders, divided into α-thalassemia (abbreviated as α-thalassemia) and β-thalassemia (β-thalassemia) two types. The disease is mainly found in the countries along the Mediterranean Sea and Southeast Asian countries. It is the single gene disease with the widest distribution and the largest cumulative population in the world. There are about 90 million symptoms and gene carriers in the world. It is more common in the south of China, and it is one of the genetic diseases with the highest incidence and the greatest impact in the provinces south of the Yangtze River in my country, especially in Guangxi, Guangdong and Hainan...

AI Technical Summary

Problems solved by technology

[0009] However, PCR amplification still has disadvantages such as relying on large instruments and slow amplification rate.

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