Humanized anti-TPBG antibody, preparation method thereof, conjugate and application thereof

A humanized and human antibody technology, applied in biochemical equipment and methods, chemical equipment and methods, botany equipment and methods, etc., can solve problems such as the limitation of killing effect and the damage of non-cancer cells
CN108690136AActive Publication Date: 2018-10-23KAIHUI SCI & TECH DEV SHANGHAI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KAIHUI SCI & TECH DEV SHANGHAI
Publication Date
2018-10-23

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Abstract

The invention discloses a humanized anti-TPBG antibody, a preparation method thereof, a conjugate thereof and application thereof. The humanized anti-TPBG antibody comprises (a) a framework region comprising residues of a human antibody framework region; and (b) one or more CDRs in a light chain variable region shown as SEQ ID NO.4 or 8 or one or more CDRs in a heavy chain variable region shown asSEQ ID NO.2 or 6. The humanized anti-TPBG antibody has a binding affinity of at least about 1 * 10<-7> M to about 1 * 10<-12>M for a human TPBG antigen. The humanized anti-TPBG antibody is conjugatedwith a small molecule compound such as MC-MMAF to obtain the conjugate which is effective in cytotoxic killing effect on TPBG positive cells. Therefore, the humanized anti-TPBG antibody is used for preparing an antibody cross-linking drug, which can effectively kill tumor cells and treat tumors.
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Description

technical field

[0001] The present invention relates to the field of antibodies, in particular to a humanized anti-TPBG antibody, its preparation method, its conjugate and its application. Background technique

[0002] A cell surface molecule, trophoblast-specific glycoprotein (TPBG, also known as 5T4), was found to be a specific protein expressed by embryonic trophoblasts when comparing embryonic stem cell trophoblasts with cancer cells. The human TPBG protein has a molecular weight of about 72kDa and contains 420 amino acids. Its N-terminal oligosaccharide structure has diversity, which can prevent proteolysis and interact with other molecules in the process of cell membrane signal transduction. The TPBG protein contains seven repeating leucine domains (LRRs), which can participate in protein-protein interactions.

[0003] The trophoblast is a layer of special embryonic stem cells between the placenta and the fetus, and TPBG is widely expressed in various trophoblast cell...

Claims

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