The invention relates to a traditional Chinese medicinal preparation having the advantages of environmental protection, no residual and long-acting sterilization desinsection. Raw materials of the traditional Chinese medicinal preparation comprise Herba Taraxaci, Quisqualis indica L., Sophora flavescens, Rhizoma Coptidis, Pericarpium Zanthoxyli, Gleditsia sinensis Lam, Aconitum carmichaeli Debx., Nux Vomica, Fructus Toosendan, Ailanthus altissima leaf, mulberry leaf, Clerodendrum inerme and a synergist. In the invention, all traditional Chinese medicines are mixed, a decoction or extraction process is utilized to obtain the effective components in the traditional Chinese medicines, alkali active substances in all the traditional Chinese medicines can effectively kill fungi, bacteria, viruses and insect pests in root tissues of crops and root soil, can prevent and treat pathogens and insects in fruit trees, vegetables, flowers and grain crops, and the addition of compound sodium nitrophenolate realizes disinfection and desinsection synergy, improves the tissue vitality of the crop roots, and improves the crop nutrition transport and the absorption rate of a fertilizer.