TPBG antibody, and preparation method, conjugate and application thereof

An antibody and sequence technology, applied in the field of TPBG antibody and its preparation, can solve the problems of non-cancerous cell damage and the limitation of killing effect.
CN106916227AActive Publication Date: 2017-07-04KAIHUI SCI & TECH DEV SHANGHAI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KAIHUI SCI & TECH DEV SHANGHAI
Publication Date
2017-07-04

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Abstract

The invention discloses a TPBG antibody, and a preparation method, a conjugate and an application thereof. The TPBG antibody comprises one or more of a heavy chain CDR1, a heavy chain CDR2 and a heavy chain CDR3 in the heavy chain variable region of the TPBG antibody, and / or one or more of a light chain CDR1, a light chain CDR2 and a light chain CDR3 of the light chain variable region of the TPBG antibody, and the amino acid sequences of all above heavy chains and light chains are respectively shown in the description. The TPBG antibody is a humanized antibody and has high affinity, and the conjugate prepared by coupling the TPBG antibody with a micro-molecular drug toxin MMAF has a cytotoxin killing effect on TPBG positive cells, so the conjugate can be used in the preparation of tumor treatment drugs.
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Description

technical field

[0001] The present invention relates to the field of antibodies, in particular to a TPBG antibody, its preparation method, its conjugate and its application. Background technique

[0002] A cell surface molecule, trophoblast-specific glycoprotein (TPBG, also known as 5T4), was found to be a specific protein expressed by embryonic trophoblasts when comparing embryonic stem cell trophoblasts with cancer cells. The human TPBG protein has a molecular weight of about 72kDa and contains 420 amino acids. Its N-terminal oligosaccharide structure has diversity, which can prevent proteolysis and interact with other molecules in the process of cell membrane signal transduction. The TPBG protein contains seven repeating leucine domains (LRRs), which can participate in protein-protein interactions.

[0003] The trophoblast is a layer of special embryonic stem cells between the placenta and the fetus, and TPBG is widely expressed in various trophoblast cells during embryo...

Claims

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