Zirconia microsphere ABO blood-type forward and reverse typing reagent card and preparation method thereof
A technology of zirconia microbeads and ABO blood type, which is applied in the field of medical testing, can solve the problems of affected centrifugal chromatography results, high non-specific adsorption, poor physical strength, etc. strong effect
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Embodiment 1
[0046] Step 1, preparation of zirconia microbead suspension medium
[0047] The formulation of the zirconia microbead suspension medium is as follows:
[0048]
[0049] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.
[0050] Step 2, preparation of zirconia microbeads
[0051] Zirconia microbeads 11 are selected, and the particle diameter is 63-125 microns. Wash with the zirconia microbead suspension medium 12 for 3-5 times to remove the broken fragments of the zirconia microbeads and aggregated zirconia microbead particles, and collect suitable zirconia microbeads with uniform particle size and complete spherical shape.
[0052] Step 3. Antibody selection
[0053] Select monoclonal anti-A antibody with IgM properties, titer ≥ 1280;
[0054] Select the monoclonal anti-B antibody with IgM properties and titer ≥1280.
[0055] Step 4, preparation of zirconia microbeads
[0056] Mix the zirconia microbeads prepared in step 2 with the antib...
Embodiment 2
[0063] Embodiment 2, preservation and detection characteristics of the reagent card of the present invention
[0064] The above-mentioned zirconia microbead ABO blood type positive and negative typing reagent card has been stored for more than 1 year. During this storage period, the zirconia microbead ABO blood type positive and negative typing reagent card has the following test results:
[0065] (1) Appearance
[0066] Zirconia microbeads The anti-A zirconia microbeads in the ABO blood type positive and negative typing reagent card are uniformly blue, the anti-B zirconia microbeads are uniformly yellow, and the zirconia microbeads suspended in the zirconia microbead suspension medium are uniformly white. There should be 2-3 mm of clear and transparent liquid at the upper end of the microbead layer, and there should be no air bubbles and foreign matter between the zirconia microbead particles.
[0067] (2) Sensitivity
[0068] In the zirconia microbead microcolumn tube cont...
Embodiment 3
[0071] Embodiment 3: the use method of the zirconia microbead ABO blood group positive and negative typing reagent card
[0072] 1. The positive and negative zirconia microbead ABO blood type determination reagent card has eight zirconia microbead microcolumn tubes. Among them, 8 zirconia microbead zirconia microbead microcolumn tubes are 2 tubes of zirconia microbead microcolumn tubes (1 / 3) containing monoclonal anti-A antibody of IgM nature, and 2 tubes of zirconia microbead microcolumn tubes of monoclonal anti-A antibody containing IgM nature. Zirconia microbead microcolumn tubes (2 / 4) for Antibody B, 4 tubes of zirconia microbead microcolumn tubes (5-8) containing zirconia microbead suspension medium for anti-setting. 2. Use low ionic strength salt solution (LISS) or normal saline to prepare the erythrocytes of the subject to a concentration of 2%, and add them to the first, second, third, and fourth microtubes respectively (two samples), each tube 20 µl.
[0073] 3. Pre...
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