Zirconia microsphere ABO blood-type forward and reverse typing reagent card and preparation method thereof

A technology of zirconia microbeads and ABO blood type, which is applied in the field of medical testing, can solve the problems of affected centrifugal chromatography results, high non-specific adsorption, poor physical strength, etc. strong effect

Active Publication Date: 2018-10-23
广东睿碁生物科技有限公司
View PDF17 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Sephadex will be affected by changes in temperature, salt concentration, osmotic pressure, and pH. The pore size of the particles will change, and the space outside the bed will also change, which will affect the results of centrifugal chroma

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Zirconia microsphere ABO blood-type forward and reverse typing reagent card and preparation method thereof
  • Zirconia microsphere ABO blood-type forward and reverse typing reagent card and preparation method thereof
  • Zirconia microsphere ABO blood-type forward and reverse typing reagent card and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Step 1, preparation of zirconia microbead suspension medium

[0047] The formulation of the zirconia microbead suspension medium is as follows:

[0048]

[0049] Dissolve the above reagents in distilled water and adjust the pH value to 6.6-6.8.

[0050] Step 2, preparation of zirconia microbeads

[0051] Zirconia microbeads 11 are selected, and the particle diameter is 63-125 microns. Wash with the zirconia microbead suspension medium 12 for 3-5 times to remove the broken fragments of the zirconia microbeads and aggregated zirconia microbead particles, and collect suitable zirconia microbeads with uniform particle size and complete spherical shape.

[0052] Step 3. Antibody selection

[0053] Select monoclonal anti-A antibody with IgM properties, titer ≥ 1280;

[0054] Select the monoclonal anti-B antibody with IgM properties and titer ≥1280.

[0055] Step 4, preparation of zirconia microbeads

[0056] Mix the zirconia microbeads prepared in step 2 with the antib...

Embodiment 2

[0063] Embodiment 2, preservation and detection characteristics of the reagent card of the present invention

[0064] The above-mentioned zirconia microbead ABO blood type positive and negative typing reagent card has been stored for more than 1 year. During this storage period, the zirconia microbead ABO blood type positive and negative typing reagent card has the following test results:

[0065] (1) Appearance

[0066] Zirconia microbeads The anti-A zirconia microbeads in the ABO blood type positive and negative typing reagent card are uniformly blue, the anti-B zirconia microbeads are uniformly yellow, and the zirconia microbeads suspended in the zirconia microbead suspension medium are uniformly white. There should be 2-3 mm of clear and transparent liquid at the upper end of the microbead layer, and there should be no air bubbles and foreign matter between the zirconia microbead particles.

[0067] (2) Sensitivity

[0068] In the zirconia microbead microcolumn tube cont...

Embodiment 3

[0071] Embodiment 3: the use method of the zirconia microbead ABO blood group positive and negative typing reagent card

[0072] 1. The positive and negative zirconia microbead ABO blood type determination reagent card has eight zirconia microbead microcolumn tubes. Among them, 8 zirconia microbead zirconia microbead microcolumn tubes are 2 tubes of zirconia microbead microcolumn tubes (1 / 3) containing monoclonal anti-A antibody of IgM nature, and 2 tubes of zirconia microbead microcolumn tubes of monoclonal anti-A antibody containing IgM nature. Zirconia microbead microcolumn tubes (2 / 4) for Antibody B, 4 tubes of zirconia microbead microcolumn tubes (5-8) containing zirconia microbead suspension medium for anti-setting. 2. Use low ionic strength salt solution (LISS) or normal saline to prepare the erythrocytes of the subject to a concentration of 2%, and add them to the first, second, third, and fourth microtubes respectively (two samples), each tube 20 µl.

[0073] 3. Pre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to a zirconia (ZrO2) microsphere ABO blood-type forward and reverse typing reagent card and a preparation method thereof. The reagent card comprises an A forward typing microcolumn tube, and the tube is filled with zirconia microsphere of a monoclonal anti-A antibody containing IgM property; a B forward typing microcolumn tube, and the tube is filled with zirconia microsphereof the monoclonal anti-A antibody containing IgM property; an A reverse typing microcolumn tube, and the tube is filled with a zirconia suspension media and zirconia microsphere; and a B reverse typing microcolumn tube, and the tube is filled with the zirconia suspension media and zirconia microsphere. The ABO blood-type forward and reverse typing reagent card has the advantages of high sensitivity, good specificity, low cost, and stable quality, and can simultaneously perform forward and reverse typing.

Description

technical field [0001] The invention relates to the field of medical detection, more specifically, the invention relates to a positive and negative zirconia microbead ABO blood type typing reagent card and a preparation method thereof. Background technique [0002] Since Landsteiner discovered the ABO blood type in 1900, blood transfusion has become one of the important medical methods to save the lives of patients. Correct identification of blood type is the first step in safe blood transfusion. ABO blood type incompatibility will lead to severe immediate hemolysis in clinical blood transfusion. Transfusion reaction, endangering the patient's life. Therefore, the accurate typing of ABO blood type is very important, which is the basis and guarantee of safe blood transfusion. [0003] The Ministry of Health issued a document in 2000 stipulating that each individual who supplies blood and receives blood must carry out ABO and Rh blood type identification (Document No. 184 (20...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/80
CPCG01N33/80
Inventor 阙秋华贺诗香周辰杰高晓玲
Owner 广东睿碁生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap