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Method for measuring volatile components of medicinal material radix curcumae by QAMS

A technology of turmeric and medicinal materials, which is applied in the field of drug detection, can solve problems affecting the reliability and practicability of the determination method of turmeric components, and achieve the effects of comprehensive coverage of medicinal material quality, improvement of production efficiency and economic benefits, and rapid method

Active Publication Date: 2018-10-30
WUXI JIYU SHANHE PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The selection of components for individual content determination will seriously affect the reliability and practicability of the method for determining the components of turmeric

Method used

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  • Method for measuring volatile components of medicinal material radix curcumae by QAMS
  • Method for measuring volatile components of medicinal material radix curcumae by QAMS
  • Method for measuring volatile components of medicinal material radix curcumae by QAMS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Step (1) The preparation method of the test solution is as follows: take 30 g of pulverized and sieved turmeric, add 1000 ml of water, extract 500 ml of aromatic water, mix well, draw 5 mL precisely, pass through a C18 solid phase extraction column, discard the effluent, and use 1mL methanol for elution, collect the eluate and dilute to 1ml, shake well, and use it as the test solution.

[0073] Step (2) Preparation of the reference substance solution: take an appropriate amount of curdione, accurately weighed, and use the external standard one-point method or the working curve method to calculate the content of curdione; wherein the external standard method uses a concentration of 50 μg / mL curdione reference substance solution.

[0074] Step (3) chromatographic conditions and system suitability test: according to the high performance liquid chromatography method;

[0075] C18 chromatographic column;

[0076] Mobile phase acetonitrile (A)-0.1% aqueous formic acid (B) ...

Embodiment 2

[0095] Step (1) The preparation method of the test solution is as follows: take 30 g of pulverized and sieved turmeric, add 1500 ml of water, extract 500 ml of aromatic water, mix well, draw 10 mL precisely, pass through a C8 solid phase extraction column, discard the effluent, and use 10mL methanol for elution, collect the eluate and dilute to 10ml, shake well, and use it as the test solution.

[0096] Step (2) Preparation of the reference substance solution: take an appropriate amount of diketone curcuma, accurately weigh it, and use the external standard one-point method or the working curve method to calculate the diketone curcuma content; the working curve adopts at least five gradients with a concentration range of 1-100 μg / mL Curcumadione reference substance solution.

[0097] Step (3) chromatographic conditions and system suitability test: according to the high performance liquid chromatography method;

[0098] C18 chromatographic column;

[0099] Mobile phase aceton...

Embodiment 3

[0118] Step (1) The preparation method of the test solution is as follows: get turmeric 30g crushed and sieved, add 1500ml of water, extract 1000ml of aromatic water, mix evenly, draw 5mL precisely, pass through a C8 solid-phase extraction small column, and discard the effluent. Elute with 1mL of methanol, collect the eluate and dilute to 10ml, shake well, and use it as the test solution.

[0119] Step (2) Preparation of the reference substance solution: take an appropriate amount of curdione, accurately weighed, and use the external standard one-point method or the working curve method to calculate the content of curdione; wherein the external standard method uses a concentration of 50 μg / mL curdione reference substance solution.

[0120] step (3)

[0121] Chromatographic conditions and system suitability test: according to the high performance liquid chromatography method;

[0122] C18 chromatographic column;

[0123] Mobile phase acetonitrile (A)-0.1% aqueous formic acid...

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Abstract

The invention discloses a method for measuring volatile components of medicinal material radix curcumae by QAMS (Quantitative Analysis of Multi-components by Single-marker). The method comprises the following steps: (1) preparation of a test liquor: taking the medicinal material radix curcumae, extracting a volatile oil by a steam distillation method, passing through a C8 or C18 stationary phase column, abandoning effluent, eluting with methanol, and collecting a methanol eluent for fixing a volume and being used as the test solution; (2) preparation of a reference liquor: taking curdione, dissolving with methanol, fixing the volume and using as the reference solution; (3) taking the test solution and the reference solution, respectively injecting into a high performance liquid chromatograph to obtain a chromatogram; and (4) first, using the reference product curdione as a reference, using a one-point external standard method or a working curve method to calculate the content of curdione in the test solution, then using a correction factor to calculate contents of curcumene ketone, curcumenol, curzerenone and germacrone. The method realizes simultaneous measurement of five sesquiterpenoids, reduces production cost, improves production efficiency and economic benefits, is fast and accurate, and can more comprehensively cover the quality of the medicinal materials.

Description

technical field [0001] The invention belongs to the field of drug detection, and in particular relates to a method for measuring the volatile components of turmeric medicinal materials with one measurement and multiple evaluations. The method includes the content determination methods of five sesquiterpene components. Background technique [0002] Due to the complexity of chemical components in natural plants, a single index component cannot accurately reflect the active ingredients of botanical medicines; and the determination of the content of a single component is formulated separately. During the implementation of the measurement method, the consumption of reference substances is large, and the detection period is long. Time-consuming and labor-intensive, the efficiency is low. [0003] Through research, it is found that the ratio of the response of different substances in the ultraviolet detector to its concentration is a constant, which is called the relative correctio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 刘薇薇甘国峰俞婷闵江夏一铜
Owner WUXI JIYU SHANHE PHARM CO LTD
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