Preparation and application of novel swine-origin fusion antimicrobial peptide VASP biological preparation

Abstract

The invention discloses preparation and application of a novel swine-origin fusion antimicrobial peptide VASP biological preparation. The fusion swine antimicrobial peptide VASP is selected from one of the following proteins (A1), (A2) or (A3): (A1) protein with an amino acid sequence being a sequence 1 in a sequence table; (A2) a protein obtained by substituting and / or deleting and / or adding oneor several amino acid residues of an amino acid sequence expressed by a sequence 2 in the sequence table and provided with the same function; and (A3) a fusion protein obtained by connecting a tag with an N terminal and / or a C terminal of (A1) or (A2). Experiments show that VASP is capable of accelerating lymphocyte proliferation and increase of the number of leukocytes, inhibiting the growth of pathogenic microorganisms, enhancing humoral immunity (IgG, IgG1 and IgG2a) and cellular immunity (CD4+, CD8+T cells), and improving the immunoregulation capability and the anti-infection survival rateof animals.

Description

technical field [0001] The invention belongs to the field of biotechnology and genetic engineering, and relates to the preparation and application of a novel porcine-derived fusion antimicrobial peptide VASP biological preparation. Background technique [0002] The main problems that restrict the further development of my country's breeding industry and improve economic benefits are the prevention and control of animal diseases, the improvement of animal product quality and the development and production of high-quality and cheap feed. my country's export of animal products accounts for a very low proportion due to quarantine and drug residue problems. For example, pork is more than 230,000 tons, accounting for only about 4% of the international trade volume. In particular, 90% of protein raw materials such as soybean meal and 70% of energy feed raw materials such as corn in Sichuan animal feed depend on external supply, which seriously restricts the development of animal hu...

AI Technical Summary

Problems solved by technology

[0005] The molecular weight of antimicrobial peptides is small, and there are certain difficulties in separation, purification and inspection

At present, the main source of antimicrobial peptides in scientific research and application is chemical synthesis, but the cost of chemical synthesis is high and the quantity is small, which is not conducive to large-scale application

Moreover, the existing single antimicrobial peptide molecule has disadvantages such as narrow antimicrobial spectrum and low biological activity; by using genetic engineering means, multiple antimicrobial peptide genes are recombined to obtain a broad-spectrum antimicrobial (G+, G-, virus, Fungi, parasites) and highly efficient recombinant antimicrobial peptide molecules with immunomodulatory activity are an important new way to overcome the defects of existing technologies and products

[0006] Reports on the fusion cloning and expression of antimicrobial peptide genes and other related genes are gradually increasing, but there are few reports on the fusion of short peptide genes with various antibacterial and immunomodulatory activities, and effective co-expression to form a variety of anti-infective active molecules and synergistic Anti-infection and Improving Immunity Level Reports

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