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A kind of identification method of wild Cordyceps sinensis and artificial Cordyceps sinensis

A technology of wild Cordyceps sinensis and Cordyceps sinensis, which is applied in the field of identification of Cordyceps sinensis products, can solve the problems of changes in the distribution of arsenic forms, which are not enough to identify wild Cordyceps sinensis, and achieve the effect of low cost

Active Publication Date: 2019-08-06
GUANGDONG MEDICAL UNIV
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Problems solved by technology

[0005] Today, the successful cultivation of artificial Cordyceps is consistent with wild Cordyceps in terms of insect species, strains, appearance and microstructure, and traditional methods (empirical methods based on morphology, color or smell) are no longer sufficient for identifying wild Cordyceps An important issue derived from artificially cultivated Cordyceps sinensis is that under the effect of artificially induced fungal infection by Cordyceps sinensis, the distribution of arsenic in the infected fungus complex will change, which has a great impact on the edible safety of Cordyceps sinensis. important

Method used

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  • A kind of identification method of wild Cordyceps sinensis and artificial Cordyceps sinensis
  • A kind of identification method of wild Cordyceps sinensis and artificial Cordyceps sinensis
  • A kind of identification method of wild Cordyceps sinensis and artificial Cordyceps sinensis

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Embodiment

[0024] (1) Sample preparation

[0025] Wild Cordyceps: Three typical areas (Litang, Nagqu and Yushu) were selected as sampling sites, and 20 samples of Cordyceps of similar size (about 0.3g each) were collected in each site. All samples were freeze-dried to constant weight. In order to collect enough material for subsequent As analysis at each sampling point, every five samples were combined into a batch of samples. These sample batches were individually crushed with a mortar and pestle to reduce particle size. Then, the fine powder was passed through a sieve with a pore size of 0.25 mm and stored in a sealed plastic bag at 4 °C until analysis.

[0026] Artificial Cordyceps sinensis: purchased from East Sunshine. Samples were randomly divided into six groups and freeze-dried to constant weight. Crush individually with a mortar and pestle to reduce particle size. Then, the fine powder was passed through a sieve with a pore size of 0.25 mm and stored in a sealed plastic bag...

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Abstract

The invention aims at providing a method for identifying wild and artificial cordyceps sinensis. Based on the routine detection of arsenic and arsenic forms in cordyceps sinensis, the content of totalarsenic, inorganic arsenic and organic arsenic in wild and artificial cordyceps sinensis is determined by inductively coupled plasma mass spectrometry (ICP-MS) and anion exchange high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The content of inorganic arsenic relative to total arsenic in wild cordyceps sinensis is lower than that in artificial cordyceps sinensis, the percentage of content of inorganic arsenic in content of total arsenic in the wild cordyceps sinensis is lower than 10%, and the content of inorganic arsenic relative to total arsenic in artificial cordyceps sinensis is higher than 30%; in the wild cordyceps sinensis, the content of unknown organic arsenic accounts for 89%-92% of that of total arsenic, and in artificial cordyceps sinensis, the content of unknown organic arsenic accounts for 51%-65% of that of total arsenic. The identification method is simple in decision, saves time and labor, and is low in cost.

Description

technical field [0001] The invention relates to a method for identifying a product of Cordyceps sinensis, in particular to a method for identifying wild Cordyceps sinensis and artificial Cordyceps sinensis. Background technique [0002] In China and surrounding countries, Cordyceps sinensis has been used as a rare functional food for more than 2,000 years to promote health and treat various chronic diseases. Cordyceps became famous around the world when Chinese female athletes broke a world record at the National Games in 1993, a feat later attributed, at least in part, to eating the fungus. Since then, more and more modern drug studies have shown that its main functions are anti-tumor, anti-inflammation, kidney protection, anti-oxidation, anti-hyperglycemia, anti-apoptosis, immune regulation and liver protection. The efficacy has led to a large demand for Cordyceps sinensis, and it has also improved the regional economic construction around the Tibetan Plateau in Qinghai a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N27/62
CPCG01N27/62G01N30/02
Inventor 郭莲仙王佳婷钟粤平王伟琪洪佳彬尚俊康林也琳
Owner GUANGDONG MEDICAL UNIV
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