31 results about "Hplc icp ms" patented technology

The invention aims at providing a method for identifying wild and artificial cordyceps sinensis. Based on the routine detection of arsenic and arsenic forms in cordyceps sinensis, the content of totalarsenic, inorganic arsenic and organic arsenic in wild and artificial cordyceps sinensis is determined by inductively coupled plasma mass spectrometry (ICP-MS) and anion exchange high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The content of inorganic arsenic relative to total arsenic in wild cordyceps sinensis is lower than that in artificial cordyceps sinensis, the percentage of content of inorganic arsenic in content of total arsenic in the wild cordyceps sinensis is lower than 10%, and the content of inorganic arsenic relative to total arsenic in artificial cordyceps sinensis is higher than 30%; in the wild cordyceps sinensis, the content of unknown organic arsenic accounts for 89%-92% of that of total arsenic, and in artificial cordyceps sinensis, the content of unknown organic arsenic accounts for 51%-65% of that of total arsenic. The identification method is simple in decision, saves time and labor, and is low in cost.

The invention discloses methods for determining total amount mercury and valence mercury content in biological tissues and organs. The method comprises the following steps: 1) pretreatment, enzymolysis, filtration and decomposition are carried out on a biological tissues sample in order, and thereby mercury is extracted; and 2) an ICP-MS method is used for determining the total amount mercury content in the biological tissues sample. The invention also discloses a method for valence mercury content in biological mechanism, the method comprises the following steps: 1) pretreatment, enzymolysis, and filtration are carried out on a biological mechanism sample in order, and thereby valence mercury is extracted; and 2) a HPLC-ICP-MS method is used for determining the valence mercury content in the biological mechanism sample. The method is simple and feasible, can effectively and completely extract the mercury in the biological mechanism, and can grantee the stability of the valence mercury.

A method for detecting a divalent cadmium ion in an aquatic product by using an HPLC-ICP-MS (High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometer) coupling technique belongs to the technical field of detection of aquatic products. The method comprises the following steps: 1), selecting instruments and equipment as well as an HPLC-ICP-MS coupling system; 2), configuring reagents and a standard solution; 3), processing a sample; 4), creating conditions for the experiment instruments; and 5), detecting the sample and calculating the results. According to the method, the cadmium in the aquatic product is extracted by neutral water in an ultrasonic way and the state of the cadmium is not changed, so that the cadmium in a free ion state and the cadmium subjected to complexation with organic matters such as protein both exist in the extract solution. In the adopted mobile phase, oxalate exerts a strong complexation effect on the cadmium ions but does not adsorb and elute stable cadmium complex in the extract solution, so that the cadmium ions and the cadmium complex in the sample can be guaranteed to be better separated. The method for detecting the divalent cadmium ion in the aquatic product by using the HPLC-ICP-MS coupling technique fills a blank in national standard that only the cadmium detection method is provided but no method for detecting the state of cadmium element is provided, therefore, the quality safety of the aquatic product and products of the aquatic product can be accurately and scientifically evaluated, meanwhile, the method is of great significance for formulating a new standard on the limit of harmful elements in the aquatic product.

The invention discloses a method for determining the content of organic selenium in a selenium-rich peanut and a product prepared from the selenium-rich peanut. The method comprises the following steps: 1) mixing a sample to be tested with enzyme to obtain a mixture; 2) sequentially performing ultrasonic-assisted extraction and constant-temperature water bath oscillation on the mixture, centrifugally separating and taking liquid supernatant and residues; performing mixture treatment on the residues to obtain liquid supernatant 1 and residues 1; 3) taking the liquid supernatant and the liquid supernatant 1 and detecting the content of organic selenium by adopting an HPLC-ICP-MS method; 4) summating the content of the organic selenium in the liquid supernatant and the liquid supernatant 1 to obtain the content of organic selenium in the sample to be tested and a selenium-rich wheat standard substance. According to the method disclosed by the invention, the content of the organic selenium in the selenium-rich peanut and the product prepared by the selenium-rich peanut can be more accurately determined.

The invention discloses methods for determining total amount arsenic and valence arsenic content in biological tissues and organs. The method comprises the following steps: 1) pretreatment, enzymolysis, filtration and decomposition are carried out on a biological tissues sample in order, and thereby arsenic is extracted; and 2) an ICP-MS method is used for determining the total amount arsenic content in the biological tissues sample. The invention also discloses a method for valence arsenic content in biological mechanism, the method comprises the following steps: 1) pretreatment, enzymolysis, and filtration are carried out on a biological mechanism sample in order, and thereby valence arsenic is extracted; and 2) a HPLC-ICP-MS method is used for determining the valence arsenic content in the biological mechanism sample. The method is simple and feasible, can effectively and completely extract the mercury in the biological mechanism, and can grantee the stability of the valence arsenic.

The invention discloses a method for detecting alkyl mercury in water based on a solid-phase extraction gathering and liquid-phase chromatograph-ICP-MS combined technology. The method comprises the following steps: a, gathering an alkyl mercury mixed standard series in a solid phase extraction manner; b, determining the gathered alkyl mercury mixed standard series by utilizing HPLC-ICP-MS to obtain a linear relation equation between the concentration of the standard series and an area of a chromatographic peak; c, gathering alkyl mercury in an unknown water sample in a solid-phase extraction manner; d, determining the gathered alkyl mercury of the unknown water sample by utilizing PLC-ICP-MS, substituting the obtained peak area into the linear equation, and calculating to obtain the concentration of the alkyl mercury in the water. By adopting the method, the linear detection range of the alkyl mercury is wide, and the detection limit is low. The method has characteristics of simplicity in operation, high accuracy, high sensitivity, high automation degree, environmental friendliness and the like and is suitable for determining alkyl mercury in surface water, water from sewage factories and water in water supply pipe network.

The invention discloses a quick determination method of speciation mercury in liquefied natural gases. The quick determination method includes steps of solution preparation, sample pretreatment, HPLC-ICP-MS instrument condition and result determination, result calculation, etc. The quick determination method of speciation mercury in liquefied natural gases is convenient, quick and accurate.