31 results about "Hplc icp ms" patented technology

A method for detecting a divalent cadmium ion in an aquatic product by using an HPLC-ICP-MS (High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometer) coupling technique belongs to the technical field of detection of aquatic products. The method comprises the following steps: 1), selecting instruments and equipment as well as an HPLC-ICP-MS coupling system; 2), configuring reagents and a standard solution; 3), processing a sample; 4), creating conditions for the experiment instruments; and 5), detecting the sample and calculating the results. According to the method, the cadmium in the aquatic product is extracted by neutral water in an ultrasonic way and the state of the cadmium is not changed, so that the cadmium in a free ion state and the cadmium subjected to complexation with organic matters such as protein both exist in the extract solution. In the adopted mobile phase, oxalate exerts a strong complexation effect on the cadmium ions but does not adsorb and elute stable cadmium complex in the extract solution, so that the cadmium ions and the cadmium complex in the sample can be guaranteed to be better separated. The method for detecting the divalent cadmium ion in the aquatic product by using the HPLC-ICP-MS coupling technique fills a blank in national standard that only the cadmium detection method is provided but no method for detecting the state of cadmium element is provided, therefore, the quality safety of the aquatic product and products of the aquatic product can be accurately and scientifically evaluated, meanwhile, the method is of great significance for formulating a new standard on the limit of harmful elements in the aquatic product.

The invention discloses a method for detecting alkyl mercury in water based on a solid-phase extraction gathering and liquid-phase chromatograph-ICP-MS combined technology. The method comprises the following steps: a, gathering an alkyl mercury mixed standard series in a solid phase extraction manner; b, determining the gathered alkyl mercury mixed standard series by utilizing HPLC-ICP-MS to obtain a linear relation equation between the concentration of the standard series and an area of a chromatographic peak; c, gathering alkyl mercury in an unknown water sample in a solid-phase extraction manner; d, determining the gathered alkyl mercury of the unknown water sample by utilizing PLC-ICP-MS, substituting the obtained peak area into the linear equation, and calculating to obtain the concentration of the alkyl mercury in the water. By adopting the method, the linear detection range of the alkyl mercury is wide, and the detection limit is low. The method has characteristics of simplicity in operation, high accuracy, high sensitivity, high automation degree, environmental friendliness and the like and is suitable for determining alkyl mercury in surface water, water from sewage factories and water in water supply pipe network.

The invention discloses a method for separating and determining chromium elements with different valences in tobacco and tobacco products. The method comprises the following steps of 1, selecting apparatuses and equipment, wherein the separation and determination device is a high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) combined device, 2, preparing a reagent and a standard solution, 3, pretreating a sample, 4, determining the sample and calculating and expressing a calculation result. The method utilizes the HPLC-ICP-MS combined technology to realize separation and determination of chromium elements with different valences in tobacco and tobacco products, has good effects of analysis, determination and separation of chromium elements with different valences, a low detecting limit, high sensitivity and good repeatability, can detect chromium elements with different valences in a sub-concentration (microgram / liter) level, and in other word, can detect content of Cr(III) and Cr(VI) in tobacco and tobacco products. The method for separation and determination optimizes a sample pre-treatment process and simplifies separation and determination processes.

The invention discloses a method for synchronously detecting a content of food additives and heavy metals in food in an inverse HPLC-ICP-MS method. The method comprises the following steps: connecting an HPLC device and an ICP-MS device by virtue of a dedicated interface system (5) of Agilent company, detecting the content of the food additives in a processed food sample by virtue of HPLC, and then inputting the food sample flowing by the HPLC into the ICP-MS to perform the heavy metal content detection by virtue of the interface system (5). By adopting the method, the content of the food additives and heavy metals in the food can be rapidly, accurately and synchronously detected, not only can the sampling amount of the sample to be detected and the pretreatment amount of the sample be reduced, but also the operation complexity caused by separate detection and separate sample feeding of different instruments can be reduced, the detection time is greatly shortened, and the working efficiency is improved. The detection time of the two instruments is respectively about 6 hours, and after the two instruments are combined to use, the time is about 4.8 hours, and the time is saved by about 20 percent.

The invention provides detecting of different arsenic forms in environmental water based on an HPLC-ICP-MS hyphenated technology. Detecting specifically comprises the steps that (1) a mixed standard solution is collocated, specifically, flowing phase L-aminothiopropionic acid serves as a solvent to collocate an arsenic mixed standard solution; (2) standard curves are drawn, specifically, the mixedstandard solution prepared from the step 1 is taken, the arsenic form concentration is detected by using the HPLC-ICP-MS, and according to the arsenic form concentration in the mixed standard solution and corresponding signal intensity, the standard curves of arsenic forms are drawn; (3) pretreatment is conducted on a sample, specifically, a to-be-detected water sample passes through a 0.45 [mu]mfilter membrane before sampling, a KOH solution is added, the sample is placed in a 4 DEG C low temperature condition after sampling, in experiment, the to-be-detected sample is measured out to a centrifuge tube, a flow phase is added to serve as a to-be-detected sample solution, and meanwhile blank test is carried out; and (4) the sample is detected, specifically, the HPLC-ICP-MS is used for measuring and analyzing the arsenic form concentration in the to-be-detected sample solution, the respective concentration value of the arsenic forms in the to-be-detected sample are calculated and analized according to RSD (relative standard deviation) of signal intensity obtained by calculating and the respective standard curves of the arsenic forms.

The invention aims at providing a method for identifying wild and artificial cordyceps sinensis. Based on the routine detection of arsenic and arsenic forms in cordyceps sinensis, the content of totalarsenic, inorganic arsenic and organic arsenic in wild and artificial cordyceps sinensis is determined by inductively coupled plasma mass spectrometry (ICP-MS) and anion exchange high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The content of inorganic arsenic relative to total arsenic in wild cordyceps sinensis is lower than that in artificial cordyceps sinensis, the percentage of content of inorganic arsenic in content of total arsenic in the wild cordyceps sinensis is lower than 10%, and the content of inorganic arsenic relative to total arsenic in artificial cordyceps sinensis is higher than 30%; in the wild cordyceps sinensis, the content of unknown organic arsenic accounts for 89%-92% of that of total arsenic, and in artificial cordyceps sinensis, the content of unknown organic arsenic accounts for 51%-65% of that of total arsenic. The identification method is simple in decision, saves time and labor, and is low in cost.

The invention discloses a determination method for arsenic of four forms in imported liquefied natural gas. The method comprises the steps of solution preparation, sample pretreatment, HPLC-ICP-MS condition and result determination, result calculation and the like. The determination method is convenient, rapid and accurate to implement.

The invention discloses methods for determining total amount mercury and valence mercury content in biological tissues and organs. The method comprises the following steps: 1) pretreatment, enzymolysis, filtration and decomposition are carried out on a biological tissues sample in order, and thereby mercury is extracted; and 2) an ICP-MS method is used for determining the total amount mercury content in the biological tissues sample. The invention also discloses a method for valence mercury content in biological mechanism, the method comprises the following steps: 1) pretreatment, enzymolysis, and filtration are carried out on a biological mechanism sample in order, and thereby valence mercury is extracted; and 2) a HPLC-ICP-MS method is used for determining the valence mercury content in the biological mechanism sample. The method is simple and feasible, can effectively and completely extract the mercury in the biological mechanism, and can grantee the stability of the valence mercury.

A method for detecting a divalent cadmium ion in an aquatic product by using an HPLC-ICP-MS (High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometer) coupling technique belongs to the technical field of detection of aquatic products. The method comprises the following steps: 1), selecting instruments and equipment as well as an HPLC-ICP-MS coupling system; 2), configuring reagents and a standard solution; 3), processing a sample; 4), creating conditions for the experiment instruments; and 5), detecting the sample and calculating the results. According to the method, the cadmium in the aquatic product is extracted by neutral water in an ultrasonic way and the state of the cadmium is not changed, so that the cadmium in a free ion state and the cadmium subjected to complexation with organic matters such as protein both exist in the extract solution. In the adopted mobile phase, oxalate exerts a strong complexation effect on the cadmium ions but does not adsorb and elute stable cadmium complex in the extract solution, so that the cadmium ions and the cadmium complex in the sample can be guaranteed to be better separated. The method for detecting the divalent cadmium ion in the aquatic product by using the HPLC-ICP-MS coupling technique fills a blank in national standard that only the cadmium detection method is provided but no method for detecting the state of cadmium element is provided, therefore, the quality safety of the aquatic product and products of the aquatic product can be accurately and scientifically evaluated, meanwhile, the method is of great significance for formulating a new standard on the limit of harmful elements in the aquatic product.

The invention discloses a method for determining the content of organic selenium in a selenium-rich peanut and a product prepared from the selenium-rich peanut. The method comprises the following steps: 1) mixing a sample to be tested with enzyme to obtain a mixture; 2) sequentially performing ultrasonic-assisted extraction and constant-temperature water bath oscillation on the mixture, centrifugally separating and taking liquid supernatant and residues; performing mixture treatment on the residues to obtain liquid supernatant 1 and residues 1; 3) taking the liquid supernatant and the liquid supernatant 1 and detecting the content of organic selenium by adopting an HPLC-ICP-MS method; 4) summating the content of the organic selenium in the liquid supernatant and the liquid supernatant 1 to obtain the content of organic selenium in the sample to be tested and a selenium-rich wheat standard substance. According to the method disclosed by the invention, the content of the organic selenium in the selenium-rich peanut and the product prepared by the selenium-rich peanut can be more accurately determined.

The invention discloses methods for determining total amount arsenic and valence arsenic content in biological tissues and organs. The method comprises the following steps: 1) pretreatment, enzymolysis, filtration and decomposition are carried out on a biological tissues sample in order, and thereby arsenic is extracted; and 2) an ICP-MS method is used for determining the total amount arsenic content in the biological tissues sample. The invention also discloses a method for valence arsenic content in biological mechanism, the method comprises the following steps: 1) pretreatment, enzymolysis, and filtration are carried out on a biological mechanism sample in order, and thereby valence arsenic is extracted; and 2) a HPLC-ICP-MS method is used for determining the valence arsenic content in the biological mechanism sample. The method is simple and feasible, can effectively and completely extract the mercury in the biological mechanism, and can grantee the stability of the valence arsenic.

The invention discloses a method for detecting alkyl mercury in water based on a solid-phase extraction gathering and liquid-phase chromatograph-ICP-MS combined technology. The method comprises the following steps: a, gathering an alkyl mercury mixed standard series in a solid phase extraction manner; b, determining the gathered alkyl mercury mixed standard series by utilizing HPLC-ICP-MS to obtain a linear relation equation between the concentration of the standard series and an area of a chromatographic peak; c, gathering alkyl mercury in an unknown water sample in a solid-phase extraction manner; d, determining the gathered alkyl mercury of the unknown water sample by utilizing PLC-ICP-MS, substituting the obtained peak area into the linear equation, and calculating to obtain the concentration of the alkyl mercury in the water. By adopting the method, the linear detection range of the alkyl mercury is wide, and the detection limit is low. The method has characteristics of simplicity in operation, high accuracy, high sensitivity, high automation degree, environmental friendliness and the like and is suitable for determining alkyl mercury in surface water, water from sewage factories and water in water supply pipe network.

The invention discloses a quick determination method of speciation mercury in liquefied natural gases. The quick determination method includes steps of solution preparation, sample pretreatment, HPLC-ICP-MS instrument condition and result determination, result calculation, etc. The quick determination method of speciation mercury in liquefied natural gases is convenient, quick and accurate.

The invention discloses a method for determining the content of soluble mercury in cinnabar-containing drugs, characterized in the following steps: (1) that the soluble mercury is extracted from the cinnabar-containing drugs by an ultrasonic treatment and / or a magnetic stir with a bionic solvent; (2) that preferably a solution extracted in step (1) is purified; and (3) that an ICP-MS method is used to determine the content of the soluble mercury in the cinnabar-containing drugs. The invention also discloses a method for determining the content of soluble valence-state mercury in the cinnabar-containing drugs. In the invention the method for determining cinnabar drug effects and main toxicity components is established by simulating extraction manners in bodies and by adopting the ICP-MS method and an HPLC-ICP-MS method. In addition, the method is sensitive and accurate.