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A method for measuring organic selenium content in selenium-enriched peanuts or products prepared therefrom

A technology of selenium-enriched peanuts and organic selenium, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of processing selenium loss, affecting the content of organic selenium, and the inability to quantify selenium compounds, so as to achieve good loss, extraction and reduction of selenium form The effect of changing and shortening the extraction time

Active Publication Date: 2018-08-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The determination of organic selenium in selenium-enriched peanuts must go through the oil removal process, but in this process, the loss of selenium must be introduced during processing.
At present, organic selenium detection methods usually use the combination of selenium monomer as the standard sample, and use the method of spiked recovery rate to evaluate the extraction rate of selenium. This type of method can only realize the evaluation of the detection of free organic selenium in the sample, but not the Quantification of selenium compounds in the bound form, thus affecting the objective reflection of the total organic selenium content in the sample

Method used

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  • A method for measuring organic selenium content in selenium-enriched peanuts or products prepared therefrom
  • A method for measuring organic selenium content in selenium-enriched peanuts or products prepared therefrom
  • A method for measuring organic selenium content in selenium-enriched peanuts or products prepared therefrom

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Experimental program
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Effect test

Embodiment 1

[0048] 1) Preparation of samples: Selenium-enriched peanut raw materials (total selenium content of 0.513mg / kg) were selected from the Shandong Boshan planting base funded by the National Public Welfare Industry (Agriculture) Special Project (No. 201303106); the selected selenium-enriched Peanuts are shelled and soaked in ultra-pure water for 5 minutes, uncoated, and dried to obtain selenium-enriched peanut kernels; pre-crushing selenium-enriched peanut kernels to obtain selenium-enriched peanut crushed is subjected to ultra-high pressure auxiliary solvent extraction, and selenium-enriched peanut powder is mixed with 6 The ratio of solvent to oil to liquid is 1:6g / mL, the ultra-high pressure treatment pressure is 300MPa, the extraction time is 10min, and the turbid liquid is shaken in a constant temperature water bath for 6h at 50°C, centrifuged at 4000r / min for 20min to obtain a cake for extraction, and the cake is extracted Constant temperature water bath leaching, centrifuga...

Embodiment 2

[0066] According to the steps of Example 1, only add deionized water to the selenium-enriched peanut defatted powder obtained in step 1), the ratio of material to water to liquid is 1:12g / mL, adjust the pH to 9.5 with NaOH (concentration: 1mol / L), 40°C Stir and extract in a constant temperature water bath for 1 h. 8) The extracted solution is subjected to vacuum filtration, the filtrate is collected, and the pH is adjusted to 4.7 with HCl (concentration: 1mol / L); centrifugation: 5000r / min, 15min, and the precipitate is taken; The pulverizer carries out ultrafine pulverization, and passes through a 150-mesh sieve to obtain selenium-enriched peanut protein. The result shows that the protein content of selenium-enriched peanut protein powder is 91%, and by the method detection of 3) content determination in embodiment 1, obtains that its organic selenium mainly exists with selenomethionine, and content (in terms of selenium) is 0.80 μ g / g, accounting for 76% of the total seleni...

Embodiment 3

[0068] According to the step of example 1, only increase step 4) measure the loss of selenium in selenium-enriched peanut raw material to selenium-enriched peanut protein powder preparation process, as figure 2 shown, combined with image 3 The results show that the selenium in the peanut is mainly distributed in the defatted powder, the peanut oil does not contain selenium, and the peanut skin contains a certain amount of selenium, but the quality of the peanut skin is small (less than 1%), and the main components are fiber, glycosides, etc.; so the loss of selenium in the steps of removing peanut shells and peanut oil does not involve the loss of selenomethionine; the drying and crushing process belongs to thermal processing, which will cause the morphological transformation of selenomethionine and cause volatilization loss, so the selenium in the drying and crushing process Loss as selenoamino acid loss. The selenium content of every hundred selenium-enriched peanut raw m...

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Abstract

The invention discloses a method for determining the content of organic selenium in a selenium-rich peanut and a product prepared from the selenium-rich peanut. The method comprises the following steps: 1) mixing a sample to be tested with enzyme to obtain a mixture; 2) sequentially performing ultrasonic-assisted extraction and constant-temperature water bath oscillation on the mixture, centrifugally separating and taking liquid supernatant and residues; performing mixture treatment on the residues to obtain liquid supernatant 1 and residues 1; 3) taking the liquid supernatant and the liquid supernatant 1 and detecting the content of organic selenium by adopting an HPLC-ICP-MS method; 4) summating the content of the organic selenium in the liquid supernatant and the liquid supernatant 1 to obtain the content of organic selenium in the sample to be tested and a selenium-rich wheat standard substance. According to the method disclosed by the invention, the content of the organic selenium in the selenium-rich peanut and the product prepared by the selenium-rich peanut can be more accurately determined.

Description

technical field [0001] The invention relates to a method for measuring organic selenium content in selenium-enriched peanuts or products prepared therefrom, and belongs to the field of food quality analysis methods. Background technique [0002] As one of the essential trace elements for the human body, selenium has a variety of biological effects, especially its physiological functions such as enhancing immunity, preventing chronic diseases and anti-cancer, and has attracted much attention. The selenium nutrition of the body is taken in through diet. And due to the limitation of environmental selenium resources (such as China has 72% of the regions are selenium deficient areas), the selenium content in natural foods in most areas is relatively low. Through the improvement of agricultural measures to increase the selenium content of crops, the biological selenium enrichment of agricultural products can be realized. Today, the selenium-enriched industry has developed with t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14
Inventor 吴继红高林胡小松罗东升
Owner CHINA AGRI UNIV
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