Method for determining the content of total arsenic and valence arsenic in biological tissues and organs

An organism and biological technology, applied in the field of determination of valence arsenic content in organism tissues and organs

Active Publication Date: 2019-07-30
SHANGHAI INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason for the current situation is that there is no effective means to accurately determine the form and valence of arsenic in the organism, and none of the methods reported in the existing literature can completely extract the arsenic in the organism. Ensure that the valence state of arsenic does not change. Since the toxicity of arsenic in different valence states is different, accurate determination of the content of arsenic in each valence state is very important for judging the mechanism of realgar efficacy and toxicity

Method used

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  • Method for determining the content of total arsenic and valence arsenic in biological tissues and organs
  • Method for determining the content of total arsenic and valence arsenic in biological tissues and organs
  • Method for determining the content of total arsenic and valence arsenic in biological tissues and organs

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Effect test

Embodiment 1

[0046] Determination of total arsenic in blood, liver, kidney, brain, heart, spleen and lung of rats: determined according to inductively coupled plasma mass spectrometry (Appendix XI D of Chinese Pharmacopoeia 2010 Edition).

[0047] Reagents and Instruments

[0048] American Agilent 7700ce inductively coupled plasma mass spectrometer; Branson Ultrasound (Shanghai) Co., Ltd. B9500S-DTH ultrasonic cleaner (power 300W, frequency 45kHz); American Millipore Milli-Q ultrapure water machine. Arsenic standard solution (1000mg / L, batch number HC961324) was purchased from Merk Company.

[0049] Both nitric acid and hydrochloric acid are superior grade pure (Merck company); pepsin is a biochemical reagent, the enzyme activity is 1:27000, and the batch number is 100710, purchased from Shanghai Huixing Biochemical Reagent Co., Ltd.; pancreatin is a biochemical reagent, and the casein conversion capacity ≥25.0, the batch number is F20100714, purchased from Sinopharm Chemical Reagent Co.,...

Embodiment 2

[0098] Determination of valence arsenic in rat blood, liver, kidney, brain, heart, spleen and lung: according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 edition) and inductively coupled plasma mass spectrometry (Appendix XI D ) determination.

[0099] Reagents and Instruments

[0100] American Agilent Company 7700ce inductively coupled plasma mass spectrometer; American Agilent Company 1100 liquid chromatograph; Branson Ultrasound (Shanghai) Co., Ltd. B9500S-DTH ultrasonic cleaning machine (power 300W, frequency 45kHz); American Millipore Company Milli -Q ultrapure water machine.

[0101] Arsenite solution standard substance [As(III), AsO 3 3- 1.011μmol / g, GBW08666, batch number 0901]; arsenate solution standard substance [As(V), AsO 4 3 -=0.233μmol / g, GBW08667, batch number 0901]; monomethyl arsenic solution standard substance [MMA, CH 3 AsO 3 2- =0.355μmol / g, GBW08668, batch number 0803]; standard substance of dimethyl arse...

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Abstract

The invention discloses methods for determining total amount arsenic and valence arsenic content in biological tissues and organs. The method comprises the following steps: 1) pretreatment, enzymolysis, filtration and decomposition are carried out on a biological tissues sample in order, and thereby arsenic is extracted; and 2) an ICP-MS method is used for determining the total amount arsenic content in the biological tissues sample. The invention also discloses a method for valence arsenic content in biological mechanism, the method comprises the following steps: 1) pretreatment, enzymolysis, and filtration are carried out on a biological mechanism sample in order, and thereby valence arsenic is extracted; and 2) a HPLC-ICP-MS method is used for determining the valence arsenic content in the biological mechanism sample. The method is simple and feasible, can effectively and completely extract the mercury in the biological mechanism, and can grantee the stability of the valence arsenic.

Description

technical field [0001] The invention relates to a method for measuring the total arsenic content in biological body tissues and organs, and also relates to a method for measuring the valence arsenic content in biological body tissues and organs. Background technique [0002] Realgar is a commonly used mineral traditional Chinese medicine in my country. It has been clinically used for thousands of years. The earliest record of its medicinal use is "Shen Nong's Herbal Classic". It can be taken internally and externally and has a wide range of uses. There are about 460 prescriptions containing realgar and cinnabar in the existing national standards; currently, there are 10 varieties containing realgar and 23 varieties containing realgar and cinnabar in the first part of the Chinese Pharmacopoeia 2010 edition. Among the varieties listed in the 2010 edition of the Chinese Pharmacopoeia, there are 72 species containing realgar, and 94 species containing realgar and cinnabar. Amon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64G01N30/02
Inventor 季申夏晶李丽敏胡青曹帅陈虹苗水毛秀红毛丹孙健周恒于泓仇佳思杨盈盈杨文婷
Owner SHANGHAI INST FOR FOOD & DRUG CONTROL
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