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Methods for determining total amount arsenic and valence arsenic content in biological tissues and organs

A body and biological technology, applied in the field of determining the content of valence arsenic in biological tissues and organs

Active Publication Date: 2016-08-17
SHANGHAI INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason for the current situation is that there is no effective means to accurately determine the form and valence of arsenic in the organism, and none of the methods reported in the existing literature can completely extract the arsenic in the organism. Ensure that the valence state of arsenic does not change. Since the toxicity of arsenic in different valence states is different, accurate determination of the content of arsenic in each valence state is very important for judging the mechanism of realgar efficacy and toxicity

Method used

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  • Methods for determining total amount arsenic and valence arsenic content in biological tissues and organs
  • Methods for determining total amount arsenic and valence arsenic content in biological tissues and organs
  • Methods for determining total amount arsenic and valence arsenic content in biological tissues and organs

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Determination of total arsenic in rat blood, liver, kidney, brain, heart, spleen and lung: According to inductively coupled plasma mass spectrometry (Appendix XI D, 2010 edition of Chinese Pharmacopoeia).

[0047] Reagents and instruments

[0048] American Agilent 7700ce inductively coupled plasma mass spectrometer; Branson Ultrasonic (Shanghai) Co., Ltd. B9500S-DTH ultrasonic cleaner (power 300W, frequency 45kHz); American Millipore company Milli-Q ultrapure water machine. Arsenic standard solution (1000mg / L, batch number HC961324), purchased from Merk Company.

[0049] Nitric acid and hydrochloric acid are both premium grade pure (Merck company); pepsin is a biochemical reagent with an enzyme activity of 1:27000, and the batch number is 100710, purchased from Shanghai Huixing Biochemical Reagent Co., Ltd.; pancreatin is a biochemical reagent, with casein conversion ability ≥25.0, batch number is F20100714, purchased from Sinopharm Chemical Reagent Co., Ltd.; water is deioni...

Embodiment 2

[0098] Determination of valence arsenic in rat blood, liver, kidney, brain, heart, spleen and lung: According to high performance liquid chromatography (Chinese Pharmacopoeia 2010 edition one Appendix VI D) and inductively coupled plasma mass spectrometry (Appendix XI D) ) Determination.

[0099] Reagents and instruments

[0100] American Agilent 7700ce inductively coupled plasma mass spectrometer; American Agilent 1100 liquid chromatograph; Branson Ultrasonic (Shanghai) Co., Ltd. B9500S-DTH ultrasonic cleaner (power 300W, frequency 45kHz); American Millipore -Q ultrapure water machine.

[0101] Standard material of arsenite solution [As(III), AsO 3 3- 1.011μmol / g, GBW08666, batch number 0901]; arsenate solution standard material [As(V), AsO 4 3 -= 0.233μmol / g, GBW08667, batch number 0901]; a standard material of methyl arsenic solution [MMA, CH 3 AsO 3 2- =0.355μmol / g, GBW08668, batch number 0803]; standard material of dimethyl arsenic solution [DMA, CH 3 CH 2 AsO 2 =0.706μmol / g, G...

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Abstract

The invention discloses a method for determining the form and valence content of arsenic in biological tissues and organs. Arsenic in it; (2) Determination of total arsenic content in biological organism samples by ICP-MS. The invention also discloses a method for measuring the content of valence arsenic in biological organisms, which is characterized in that the method comprises the steps: (1) the biological organism samples are sequentially pretreated, enzymatically hydrolyzed and filtered, thereby extracting the valence arsenic therein; ( 2) Determination of valence arsenic content in biological organism samples by HPLC-ICP-MS method. The method is simple and feasible, not only can effectively and completely extract the arsenic in the biological body, but also can ensure the stability of the valence arsenic.

Description

Technical field [0001] The invention relates to a method for determining the total arsenic content in biological tissues and organs. The invention also relates to a method for determining the valence arsenic content in biological tissues and organs. Background technique [0002] Realgar is a commonly used mineral Chinese medicine in my country. It has a history of clinical application for thousands of years. The earliest recorded medicine is "Shen Nong's Materia Medica", which can be used internally and externally and has a wide range of uses. The existing national standards include about 460 kinds of prescription preparations containing realgar and cinnabar; currently, there are 10 realgar-containing varieties and 23 realgar and cinnabar-containing varieties included in the 2010 edition of the Chinese Pharmacopoeia. Among the varieties in the 2010 edition of the Chinese Pharmacopoeia, 72 species contain realgar and 94 species contain realgar and cinnabar. Among them, there are ...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N30/02
Inventor 季申夏晶李丽敏胡青曹帅陈虹苗水毛秀红毛丹孙健周恒于泓仇佳思杨盈盈杨文婷
Owner SHANGHAI INST FOR FOOD & DRUG CONTROL
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