Methods for determining total amount mercury and valence mercury content in biological tissues and organs

An organism and biological technology, applied in the field of determination of valence mercury content in organism tissues and organs, can solve the problem of insufficient poisoning and increase the chance of poisoning, and achieve the effect of reliable data support

An organism and biological technology, applied in the field of determination of valence mercury content in organism tissues and organs, can solve the problem of insufficient poisoning and increase the chance of poisoning, and achieve the effect of reliable data support

CN105866232AActive Publication Date: 2016-08-17SHANGHAI INST FOR FOOD & DRUG CONTROL
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  • Methods for determining total amount mercury and valence mercury content in biological tissues and organs
  • Methods for determining total amount mercury and valence mercury content in biological tissues and organs
  • Methods for determining total amount mercury and valence mercury content in biological tissues and organs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Determination of total mercury in blood, liver, kidney, brain, heart, spleen and lung of rats: determined according to inductively coupled plasma mass spectrometry (Appendix XI D of Chinese Pharmacopoeia 2010 Edition).

[0047] Reagents and Instruments

[0048] American Agilent 7700ce inductively coupled plasma mass spectrometer; Branson Ultrasound (Shanghai) Co., Ltd. B9500S-DTH ultrasonic cleaner (power 300W, frequency 45kHz); American Millipore Milli-Q ultrapure water machine. Mercury standard solution (1000mg / L, batch number HC931554) was purchased from Merk Company.

[0049] Both nitric acid and hydrochloric acid are superior grade pure (Merck company); pepsin is a biochemical reagent, the enzyme activity is 1:27000, and the batch number is 100710, purchased from Shanghai Huixing Biochemical Reagent Co., Ltd.; pancreatin is a biochemical reagent, and the casein conversion capacity ≥25.0, the batch number is F20100714, purchased from Sinopharm Chemical Reagent Co.,...

Embodiment 2

[0098] Determination of valence mercury in rat blood, liver, kidney, brain, heart, spleen and lung: according to high performance liquid chromatography (Appendix VI D of Chinese Pharmacopoeia 2010 edition) and inductively coupled plasma mass spectrometry (Appendix XI D ) determination.

[0099] Reagents and Instruments

[0100] American Agilent Company 7700ce inductively coupled plasma mass spectrometer; American Agilent Company 1100 liquid chromatograph; Branson Ultrasound (Shanghai) Co., Ltd. B9500S-DTH ultrasonic cleaning machine (power 300W, frequency 45kHz); American Millipore Company Milli -Q ultrapure water machine.

[0101] Methylmercury chloride (C15100000, 50301, purity 98.5%), ethylmercury chloride (C13345000, 50407, purity 98.0%) divalent mercury (High Purity Standards, 53916, purity 99.9995%) were purchased from Germany Dr. Ehrenstrorfer).

[0102] Nitric acid and hydrochloric acid were all of superior grade, purchased from Merck, Germany; cysteine, ammonium ac...

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Abstract

The invention discloses methods for determining total amount mercury and valence mercury content in biological tissues and organs. The method comprises the following steps: 1) pretreatment, enzymolysis, filtration and decomposition are carried out on a biological tissues sample in order, and thereby mercury is extracted; and 2) an ICP-MS method is used for determining the total amount mercury content in the biological tissues sample. The invention also discloses a method for valence mercury content in biological mechanism, the method comprises the following steps: 1) pretreatment, enzymolysis, and filtration are carried out on a biological mechanism sample in order, and thereby valence mercury is extracted; and 2) a HPLC-ICP-MS method is used for determining the valence mercury content in the biological mechanism sample. The method is simple and feasible, can effectively and completely extract the mercury in the biological mechanism, and can grantee the stability of the valence mercury.

Description

technical field [0001] The invention relates to a method for measuring the total mercury content in biological body tissues and organs, and also relates to a method for measuring the valence mercury content in biological body tissues and organs. Background technique [0002] Cinnabar is a commonly used mineral traditional Chinese medicine in my country. It has a history of clinical application for thousands of years. The earliest record of its use as medicine is "Shen Nong's Herbal Classic". It can be taken internally and externally and has a wide range of uses. There are about 460 prescription preparations containing cinnabar in the existing national standards; at present, there are 34 varieties containing cinnabar and 23 varieties containing realgar and cinnabar in the first part of the Chinese Pharmacopoeia 2010 edition, which have not been recorded in China. Among the varieties in the 2010 edition of the Pharmacopoeia, 227 kinds contain cinnabar, and 94 kinds contain rea...

Claims

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Application Information

Patent Timeline
17 Aug 2016
Publication
CN105866232A
IPC
G01N27/64; G01N30/02
Inventors
夏晶; 季申