Ultrasonic assisted HPLC-ICP-MS detecting and analyzing method for different arsenic form component content in complementary foods for infants

Abstract

The invention discloses an ultrasonic assisted HPLC-ICP-MS detecting and analyzing method for different arsenic form component content in complementary foods for infants. The method comprises the following steps: 1) after the to-be-detected sample of complementary foods for infants is pretreated, extracting by using a methanol aqueous solution and an enzyme solution to obtain a to-be-measured liquid; 2) separating different arsenic form components of the to-be-detected liquid by using high performance liquid chromatography; and 3) measuring different arsenic form components by using the CCT mode of ICP-MS. According to the ultrasonic assisted HPLC-ICP-MS detecting and analyzing method for different arsenic form component content in complementary foods for infants, an arsenic form liquid chromatography-inductively coupled plasma mass spectrometry combined analysis method is established, the extracting method and chromatographic conditions of the arsenic form in various types of complementary foods for infants are optimized. The method is suitable for actual detection of various types of complementary foods for infants and has the advantages of being efficient, fast, accurate and thelike.

Description

technical field [0001] The invention relates to the technical field of detection and analysis of complementary foods for infants, in particular to an ultrasonic-assisted HPLC-ICP-MS detection and analysis method for the content of different arsenic components in complementary foods for infants. Background technique [0002] Supplementary food for infants is a food that must be added during the growth and development of infants (from about 6 months old) to ensure the daily nutritional intake, but it is also an important way for infants to be exposed to heavy metal pollution. The raw materials of cereal supplementary foods for infants and young children are rice or oats, etc., which have different degrees of enrichment for heavy metals in the soil, especially rice, which is about 10 times higher than barley or wheat in arsenic enrichment capacity; It is animal-derived muscle or fish and shrimp in water, and it also has a strong enrichment effect on heavy metals in the environm...

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Problems solved by technology

In the prior art, for example, the Chinese invention patent with publication number CN 106908530A discloses a method and application of IC-ICP-MS for the determination of arsenic form in tea leaves. This method utilizes ion chromatography to study 4 kinds of arsenic in tea leaves. Morphological substances, the pretreatment uses 0.2mol / L nitric acid aqueous solution to digest at 100°C for 2 hours, but when this method is applied to infant rice noodles, noodles and other sample matrices, it cannot be extracted due to gelatinization reaction; for example, the publication number is The Chinese invention patent of CN 108802219 A discloses a method for identifying wild Cordyceps sinensis and artificial Cordyceps sinensis. The method studies five arsenic forms in Cordyceps sinensis. On the other hand, it is easy to cause the transformation of different forms of arsenic during the extraction process

[0006] Supplementary foods for infants and young children include rice noodles, noodles, rice cakes, fish paste, meat paste, etc., among which rice noodles, noodles and rice cake samples contain high content of starch, so it is very important to control the temperature during the pretreatment process of the experiment If the temperature exceeds 40°C, the rice flour will become gelatinized, which greatly affects the extraction efficiency of arsenic substances; the meat paste matrix contains high content of fat and protein, so the proportion of the organic phase is very important in the pretreatment process. , if the ratio is too high, the proposed fat will not only affect the extraction rate, but also lead to a decrease in column efficiency, and if the ratio is too low, it will not be able to effectively extract

Due to the complexity of such matrices as infant food supplements and the requirement to obtain low detection and quantification limits, the detection and analysis of various arsenic forms in infant food supplements is still a difficult point

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