Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Induction and Genetic Transformation of the Hairy Roots of Cinnamomum sinensis

A genetic transformation method and hairy root technology, which are applied in the field of induction of hairy roots of A. chinensis and their genetic transformation, and can solve the problems of no induced genetic transformation of hairy roots of A. chinensis, no genetic transformation of A. To achieve the effect of easy selection

Active Publication Date: 2022-02-08
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) have not yet seen the research report on the induction of the hairy roots of A. argentina and its genetic transformation, nor the research report on the genetic transformation (transgenic) of A. arborinae

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Induction and Genetic Transformation of the Hairy Roots of Cinnamomum sinensis
  • Induction and Genetic Transformation of the Hairy Roots of Cinnamomum sinensis
  • Induction and Genetic Transformation of the Hairy Roots of Cinnamomum sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] The embodiment of the present invention provides a method for inducing the efficiency of the hairy root of A. sinensis, comprising the following steps:

[0091] 1. Acquisition of exogenous

[0092] 1. Rinse the seeds with distilled water and peel off the seed coat;

[0093] 2. Soak the seeds in 75% ethanol for 30-50 sec, 20% sodium hypochlorite (NaClO) solution for 15 min, rinse with sterile water for 3-5 times, and dry the water with sterile filter paper on a clean bench;

[0094] 3. The treated seeds were placed on solid MS medium and cultured at 25°C under 8h light / 16h dark conditions;

[0095] 4. After being cultivated for 3 days, the seeds will start to germinate and be used as explant materials for subsequent infection.

[0096] 2. Agrobacterium rhizogenes infection (hairy root induction)

[0097] 1. Agrobacterium rhizogenes was cultured at 28°C in LB liquid medium containing 50mg / L Rif.

[0098] 2. Collect the bacteria by centrifugation, and resuspend the pel...

Embodiment 2

[0125] The embodiment of the present invention provides a method for inducing the efficiency of the hairy root of A. sinensis, comprising the following steps:

[0126] 1. Acquisition of exogenous

[0127] 1. Rinse the seeds with distilled water and peel off the seed coat;

[0128] 2. Soak the seeds in 75% ethanol for 30-50 sec, 20% sodium hypochlorite (NaClO) solution for 15 min, rinse with sterile water for 3-5 times, and dry the water with sterile filter paper on a clean bench;

[0129] 3. The treated seeds were placed on solid MS medium and cultured at 25°C under 8h light / 16h dark conditions;

[0130] 4. After being cultivated for 3 days, the seeds will start to germinate and be used as explant materials for subsequent infection.

[0131] 2. Agrobacterium rhizogenes infection (hairy root induction)

[0132] 1. Agrobacterium rhizogenes was cultured at 28°C in LB liquid medium containing 50mg / L Rif.

[0133] 2. Collect the bacteria by centrifugation, and resuspend the pel...

Embodiment 3

[0143] The embodiment of the present invention provides a method for inducing the efficiency of the hairy root of A. sinensis, comprising the following steps:

[0144] 1. Acquisition of exogenous

[0145] 1. Rinse the seeds with distilled water and peel off the seed coat;

[0146] 2. Soak the seeds in 75% ethanol for 30-50 sec, 20% sodium hypochlorite (NaClO) solution for 15 min, rinse with sterile water for 3-5 times, and dry the water with sterile filter paper on a clean bench;

[0147] 3. The treated seeds were placed on solid MS medium and cultured at 25°C under 8h light / 16h dark conditions;

[0148] 4. After being cultivated for 3 days, the seeds will start to germinate and be used as explant materials for subsequent infection.

[0149] 2. Agrobacterium rhizogenes infection (hairy root induction)

[0150] 1. Agrobacterium rhizogenes was cultured at 28°C in LB liquid medium containing 50mg / L Rif.

[0151] 2. Collect the bacteria by centrifugation, and resuspend the pel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for inducing and genetically transforming the hairy roots of A. sinensis. The present invention is based on the exploration of the conditions such as the type of exocarp, the infection mode, the infection time, and the hairy root culture temperature, and screens out the conditions suitable for the induction and cultivation of the hairy roots of A. suitable infestation conditions. And the optimal induction conditions for SW101 to induce hairy roots in different parts (cotyledons, stems, roots, leaves) of A. sinensis were given. The method for the genetic transformation efficiency of the hairy root of A. chinensis provided by the present invention, by introducing the green fluorescent protein gene (gfp) into the hairy root of A. argentina, visually identifies whether the genetic transformation of the hairy root of A. argentina is successful, and at the same time combines PCR The detection method double-checks the effectiveness of the transformation system. It provides a better, more efficient and simpler option for the research on the functional genes of A. argentina and / or the industrialization of the metabolites of A. arborica hairy roots.

Description

technical field [0001] In the field of biological technology, the invention specifically relates to a method for inducing and genetically transforming the hairy roots of A. sinensis. Background technique [0002] Hairy roots, also known as hairy roots, hairy roots, are inserted into the nuclear genome of plants or organs, tissues (including callus), single cells, and protoplasts by T-DNA on the Ri plasmid contained in Agrobacterium rhizogenes , and a large number of by-products are produced at the site of infection. The induced culture of hairy roots can be used as a bioreactor to produce valuable plant metabolites. In addition, exogenous genes can also be introduced by Agrobacterium rhizogenes to regulate the synthesis of target metabolites. [0003] Agarwood is formed when the trunks or roots of Aquilaria spp. and Gyrinops spp. plants in the family Aquilaria spp. secrete special oils during the self-defense response after various injuries. Both Agarwood and Pseudo-Agarw...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82A01H5/06A01H4/00
CPCA01H4/005C12N15/8205
Inventor 魏建和隋春徐娇
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com