A Simplified Method for Producing Rapeseed Male Sterile Hybrid F1 Seeds

A technology of male sterility and production methods, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of high labor cost, time-consuming and laborious, etc., and achieve the effect of saving labor cost, reducing risk, and simple operation

Active Publication Date: 2021-12-14
武汉联农种业科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the existing methods are time-consuming and labor-intensive, and the labor cost is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Breeding of Polyma cytoplasmic male sterility restorer line 7-5SR with self-incompatibility

[0018] (1) The restorer line 7-5 of Huayouza 12 was used as the female parent (genotype bnS-1300bnS-1300) and the self-incompatible single plant (genotype bnS-1300bnS-1300) isolated from the F2 progeny of Huayouza 95 F1 was obtained by hybridizing for BnS-1300BnS-1300).

[0019] (2) Using the above F1 as the female parent to backcross with 7-5 to obtain BC1.

[0020] (3) DNA molecular detection was carried out on the above-mentioned BC1 population single plant with DNA molecular markers linked to the self-incompatibility gene BnS-1300, and the self-incompatibility gene locus was screened to be a heterozygous genotype (BnS-1300bnS -1300) single plant.

[0021] (4) Using the above heterozygous single plant as the male parent and backcrossing with 7-5 to obtain BC2.

[0022] (5) DNA molecular detection was carried out on the above BC2 population single plant with DNA...

Embodiment 2

[0029] Example 2: Rape cytoplasmic male sterility hybrid seed production

[0030] The method of the present invention comprises utilizing the cytoplasmic male sterile line 616A and the restorer line 7-5SR with self-incompatibility to produce hybrid F1 seeds, and the specific steps of the hybrid seed production method include:

[0031] (1) The seeds of the cytoplasmic male sterile line 616A and the restorer line 7-5SR with self-incompatibility were mixed uniformly in a ratio of 80%: 20%.

[0032] (2) Under the isolation condition of artificial net room, the above-mentioned uniformly mixed seeds are planted with a row spacing of 20 cm and a plant spacing of 10 cm.

[0033] (3) When the seeds are mature, the sterile line and the restorer line are mixed and harvested. Statistics show that the hybrid purity is 92.6%.

Embodiment 3

[0034] Example 3: Rape Cytoplasmic Male Sterility Hybrid Seed Production

[0035] (1) The seeds of the nuclear male sterile line 616A and the restorer line 7-5SR with self-incompatibility were mixed uniformly in a ratio of 80%: 20%.

[0036] (2) Under natural isolation conditions, the above-mentioned uniformly mixed seeds are planted at a density of 25,000 plants / mu.

[0037] (3) When the seeds are mature, the sterile line and the restorer line are mixed and harvested. Statistics show that the hybrid purity is 90.6%.

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PUM

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Abstract

The present invention provides a simplified method for producing rapeseed male sterile hybrid F1 seeds, which comprises selecting a cytoplasmic male sterile line (or nuclear male sterile line) and a restorer line with self-incompatibility to produce hybrid F1 The hybrid seed production method of seeds, its concrete method is, under isolation condition, cytoplasmic male sterile line (or nuclear male sterile line) and restorer line seed with self-incompatibility characteristic are mixed according to specific ratio, will mix The uniform seeds are sown in the field of the rapeseed production area, and the sterile line and the restorer line are mixed when they are mature to produce hybrid F1 seeds. The breeding procedure of the invention is simple, saves labor cost, saves money and labor.

Description

technical field [0001] The invention belongs to the technical field of rapeseed hybrid F1 seed production, and in particular relates to a hybrid seed production for producing hybrid F1 seeds by utilizing cytoplasmic male sterile lines or nuclear male sterile lines and restorer lines with self-incompatibility characteristics method. Background technique [0002] The heterosis of rapeseed is obvious, generally can increase the yield by 20%-30%, and the yield increase rate of good combination can reach 50%. The popularization and application of hybrid rapeseed is developing rapidly. Only the area of ​​hybrid rapeseed in my country has accounted for more than 70% of the total area of ​​rapeseed. The utilization of heterosis is an important way to improve crop yield. There are many ways to utilize heterosis in rapeseed, mainly including cytoplasmic male sterility, nuclear male sterility, chemical male sterility and so on. [0003] Rape male sterility includes cytoplasmic male s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 杨光圣董发明辛强洪登峰
Owner 武汉联农种业科技有限责任公司
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