A Simplified Method for Producing Rapeseed Male Sterile Hybrid F1 Seeds
A technology of male sterility and production methods, applied in botany equipment and methods, plant gene improvement, application, etc., can solve the problems of high labor cost, time-consuming and laborious, etc., and achieve the effect of saving labor cost, reducing risk, and simple operation
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Embodiment 1
[0017] Example 1: Breeding of Polyma cytoplasmic male sterility restorer line 7-5SR with self-incompatibility
[0018] (1) The restorer line 7-5 of Huayouza 12 was used as the female parent (genotype bnS-1300bnS-1300) and the self-incompatible single plant (genotype bnS-1300bnS-1300) isolated from the F2 progeny of Huayouza 95 F1 was obtained by hybridizing for BnS-1300BnS-1300).
[0019] (2) Using the above F1 as the female parent to backcross with 7-5 to obtain BC1.
[0020] (3) DNA molecular detection was carried out on the above-mentioned BC1 population single plant with DNA molecular markers linked to the self-incompatibility gene BnS-1300, and the self-incompatibility gene locus was screened to be a heterozygous genotype (BnS-1300bnS -1300) single plant.
[0021] (4) Using the above heterozygous single plant as the male parent and backcrossing with 7-5 to obtain BC2.
[0022] (5) DNA molecular detection was carried out on the above BC2 population single plant with DNA...
Embodiment 2
[0029] Example 2: Rape cytoplasmic male sterility hybrid seed production
[0030] The method of the present invention comprises utilizing the cytoplasmic male sterile line 616A and the restorer line 7-5SR with self-incompatibility to produce hybrid F1 seeds, and the specific steps of the hybrid seed production method include:
[0031] (1) The seeds of the cytoplasmic male sterile line 616A and the restorer line 7-5SR with self-incompatibility were mixed uniformly in a ratio of 80%: 20%.
[0032] (2) Under the isolation condition of artificial net room, the above-mentioned uniformly mixed seeds are planted with a row spacing of 20 cm and a plant spacing of 10 cm.
[0033] (3) When the seeds are mature, the sterile line and the restorer line are mixed and harvested. Statistics show that the hybrid purity is 92.6%.
Embodiment 3
[0034] Example 3: Rape Cytoplasmic Male Sterility Hybrid Seed Production
[0035] (1) The seeds of the nuclear male sterile line 616A and the restorer line 7-5SR with self-incompatibility were mixed uniformly in a ratio of 80%: 20%.
[0036] (2) Under natural isolation conditions, the above-mentioned uniformly mixed seeds are planted at a density of 25,000 plants / mu.
[0037] (3) When the seeds are mature, the sterile line and the restorer line are mixed and harvested. Statistics show that the hybrid purity is 90.6%.
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