A kind of Pseudomonas fluorescens and its application
A technology of Pseudomonas fluorescens and microbial strains, applied in the field of microorganisms, can solve problems such as soil nutrient imbalance, soil quality degradation, and easy solidification of phosphate fertilizers, and achieve the effects of increasing yield and promoting plant growth.
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[0033] Screening methods for this strain include:
[0034] Step 1, primary screening and purification:
[0035] About 100 g of soil samples were collected from Ziwei Garden, Baisha Road, Bowen Road Square, and Ganjiazhai in Yanta District, Xi'an City. 100-mesh sieve, accurately weigh 10g of the soil that passed through the 100-mesh sieve in 90mL of sterile water in an aseptic manner, let it stand for 30min, shake it on a shaker at room temperature for 30min, and obtain a dilution of 10 -1 soil suspension; suck out 1mL from the above soil suspension, add 9mL sterile water, and mix thoroughly to obtain a dilution of 10 -2 Soil suspension: by analogy, the dilution is 10 -3 、10 -4 and 10 -5 soil suspension; take the above dilution as 10 -1 、10 -2 、10 -3 、10 -4 and 10 -50.1mL each of the soil suspension was spread on the inorganic phosphorus solid plate medium, and placed in a constant temperature incubator at 30°C for 3-7 days, and the strains with phosphorus-dissolving c...
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