A kind of Pseudomonas fluorescens and its application

A technology of Pseudomonas fluorescens and microbial strains, applied in the field of microorganisms, can solve problems such as soil nutrient imbalance, soil quality degradation, and easy solidification of phosphate fertilizers, and achieve the effects of increasing yield and promoting plant growth.

Active Publication Date: 2022-01-28
XIAN UNVERSITY OF ARTS & SCI
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Problems solved by technology

The application of phosphorus fertilizer is the main way to ensure the yield in agricultural production, but phosphorus fertilizer is easily solidified in the soil, and most of the phosphorus and Ca in the soil 2+ , Fe 2+ , Fe 3+ 、Al 3+ Combined to form insoluble inorganic phosphorus, so more than 95% of the phosphorus in the soil exists in an ineffective form that plants cannot directly use, and the seasonal utilization rate of phosphorus fertilizer is only 5% to 25%.
At present, in the process of agricultural production, excessive application of chemical fertilizers in order to increase production leads to soil nutritional imbalance and soil quality degradation, which seriously affects the sustainable development of my country's agriculture.

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  • A kind of Pseudomonas fluorescens and its application
  • A kind of Pseudomonas fluorescens and its application

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Embodiment Construction

[0033] Screening methods for this strain include:

[0034] Step 1, primary screening and purification:

[0035] About 100 g of soil samples were collected from Ziwei Garden, Baisha Road, Bowen Road Square, and Ganjiazhai in Yanta District, Xi'an City. 100-mesh sieve, accurately weigh 10g of the soil that passed through the 100-mesh sieve in 90mL of sterile water in an aseptic manner, let it stand for 30min, shake it on a shaker at room temperature for 30min, and obtain a dilution of 10 -1 soil suspension; suck out 1mL from the above soil suspension, add 9mL sterile water, and mix thoroughly to obtain a dilution of 10 -2 Soil suspension: by analogy, the dilution is 10 -3 、10 -4 and 10 -5 soil suspension; take the above dilution as 10 -1 、10 -2 、10 -3 、10 -4 and 10 -50.1mL each of the soil suspension was spread on the inorganic phosphorus solid plate medium, and placed in a constant temperature incubator at 30°C for 3-7 days, and the strains with phosphorus-dissolving c...

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Abstract

The invention discloses Pseudomonas fluorescens RQ3‑01, the preservation number of which is CGMCC No. 14471. In addition, the invention also discloses the application of the Pseudomonas fluorescens in degrading insoluble inorganic phosphorus. The Pseudomonas fluorescens of the present invention is a dominant bacterial strain screened out with insoluble inorganic phosphorus as a phosphorus source, and the bacterial strain converts insoluble inorganic phosphorus into bioavailable soluble phosphate during the growth and reproduction process; in addition, The Pseudomonas fluorescens of the invention also has the function of degrading organic phosphorus and potassium, can effectively increase the content of available phosphorus and available potassium in soil, and has the functions of promoting plant growth and increasing yield.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a pseudomonas fluorescens and an application thereof. Background technique [0002] Phosphorus is the main component of compounds in plants and is necessary for biological nitrogen fixation. Plant growth and photosynthesis are inseparable from the participation of phosphorus, and it is one of the essential nutrients for plant growth. The application of phosphorus fertilizer is the main way to ensure the yield in agricultural production, but phosphorus fertilizer is easily solidified in the soil, and most of the phosphorus and Ca in the soil 2+ , Fe 2+ , Fe 3+ 、Al 3+ Combined to form insoluble inorganic phosphorus, so more than 95% of the phosphorus in the soil exists in an ineffective form that plants cannot directly use, and the seasonal utilization rate of phosphorus fertilizer is only 5% to 25%. At present, in the process of agricultural production, exce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/39
CPCB09C1/10C12R2001/39C12N1/205
Inventor 赵咏梅杨银银王华高宁
Owner XIAN UNVERSITY OF ARTS & SCI
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