Method for establishing PDX model of high leukocyte leukemia

Abstract

The invention belongs to the technical field of animal models, and particularly relates to a method for establishing a PDX model of high leukocyte leukemia, and the method comprises: screening a suitable clinical patient with the high leukocyte leukemia; using a COM. TEC blood cell separator to separate leukocytes from peripheral blood; separating and purifying mononuclear cells of the leukocytescollected from the peripheral blood for cell culturing to obtain leukemia cell suspension; preparing the PDX model of high leukocyte leukemia, to be more specific, firstly using X-ray for irradiationof mice, injecting the leukemia cell suspension to caudal veins of the mice, one week later, drawing blood of tails of the mice randomly for detection, and judging whether the model is successfully established according to detection results. The method for establishing the PDX model of the high leukocyte leukemia has a high success rate of modeling, disease manifestation and cell morphology of theconstructed PDX model of the mice can directly reflect the characteristics of high leukocyte leukemia cells.

Description

technical field [0001] The invention belongs to the technical field of animal models, and in particular relates to a method for establishing a hyperleukocytic leukemia PDX model. Background technique [0002] Leukemia is the most common malignant tumor of the blood system. It is a kind of clonal proliferation disease with abnormal hematopoietic stem cells, and the prognosis is poor. Hyperleukocytic Leukemia (HLL) refers to the abnormal increase of peripheral blood leukocytes (≥100×10 9 / L) high-risk type of leukemia. Abnormal increase in leukocytes produces leukocyte stasis and blood vessel wall infiltration, which is particularly prone to respiratory distress syndrome and intracranial hemorrhage, while the remission rate of chemotherapy is low, and tumor lysis syndrome is prone to occur. However, the current research on hyperleukocytic leukemia is still in its infancy. We have found in clinical work that some patients with hyperleukocytic leukemia have a high degree of ma...

AI Technical Summary

Problems solved by technology

Abnormal increase in leukocytes produces leukocyte stasis and infiltration of blood vessel walls, especially prone to respiratory distress syndrome and intracranial hemorrhage, while the remission rate of chemotherapy is low, and tumor lysis syndrome is prone to occur

However, the current research on hyperleukocytic leukemia is still in its infancy. We have found in clinical work that some patients with hyperleukocytic leukemia have a high degree of malignancy, are prone to relapse, and have poor radiotherapy and chemotherapy effects. These valuable clinical cases Can provide an excellent basis for scientific research on leukemia

Mice are ideal animal models for studying leukemia. In recent years, it has been reported that human leukemia cells were transplanted into mice to construct PDX mouse models. However, due to the shortcomings of small number of cells and low purity of cells, the modeling rate is very low.

In addition, if leukemia cells are cultured and expanded for a long time, it is easy to cause cell gene and immune phenotype variation

Therefore, there is no report on the establishment of a PDX model of hyperleukocytic leukemia at home and abroad.

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