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The method of distinguishing honeysuckle and mountain silver flower by high performance liquid chromatography
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A high-performance liquid chromatography and silver flower technology, which is applied in the field of identification of Chinese medicinal materials source varieties, can solve the problems of large errors, many influencing factors, and insufficient testing speed.
Active Publication Date: 2022-01-11
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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This method requires the preparations containing Japonica japonicus or the preparation containing Japonica japonicus raw materials as a control to construct an acute oral inflammation model; there are defects such as complex testing methods, not fast enough testing, high cost, many influencing factors, and large errors
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[0039] Acetonitrile (FisherScientific) is chromatographic grade, water is first-class ultrapure water, and other reagents such as absolute ethanol (COMIO) are analytical grade; the reference substance of bromostrychnic acid was purchased from Shanghai Shidande Standard Technical Service Co., Ltd., batch number : 60077-46-5.
[0040] 2. Test method
[0041] 2.1 Chromatographic conditions
[0042] Chromatographic column C18, 4.6mm×250mm, 5μm; wavelength 240nm; acetonitrile as mobile phase A, phosphoric acid aqueous solution with a pH value of 2.0 as mobile phase B, using gradient elution, wherei...
Embodiment 2
[0065] Embodiment 2 verification experiment
[0066] 1. Verification of mountain silver flowers from different origins.
[0067] Taking different places of origin as the research object, the detection conditions of the method described in Example 1 are used to measure the content, and the results are shown in Figure 4 .
[0068] From top to bottom, the spectra in the figure are Chongqing Jiefang Road Silver Flower, Shandong Poly provides Guizhou Silver Flower, Shandong Poly provides Guizhou White Silver Flower, Shandong Poly provides Hunan White Silver Flower.
[0069] Combining the control spectrum of Sesau-Strychnic Acid and Figure 4 It can be seen that there is no or a small amount of chromatographic peaks at the positions corresponding to the interrupted strychnolic acid reference substances in the spectral spectrum of different origins of Sanyinhua, and the calculated content is far lower than 1.0%.
[0070] 2. Verification of Mountain Silver Flower under conditions ...
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Abstract
The invention discloses a method for identifying honeysuckle and mountain silver-flowers by using high-performance liquid chromatography. The percentage by weight of the dry product of the test object interrupted strychnic acid is used to identify honeysuckle and mountain silver flower. When the content of interrupted strychnic acid in the dry product of the test object is less than 1.5%, it is determined to be non-honeysuckle; the content of broken strychnic acid is greater than 1.0%. %, judged to be non-Yangyinhua. When the content of broken strychnic acid is less than or equal to 1.5%, and greater than or equal to 1.0%, it is judged as a mixture of honeysuckle or mountain silver flower. The present invention detects the weight percentage of interrupted strychnic acid in honeysuckle and mountain silver flower dry products by high performance liquid chromatography to identify honeysuckle and mountain silver flower, which is fast and accurate, and provides an identification method for the production, circulation and use of medicines. basis of truth.
Description
technical field [0001] The invention belongs to the technical field of identification of source varieties of traditional Chinese medicinal materials, and relates to a method for identifying honeysuckle and mountain silver-flowers, in particular to a method for identifying honeysuckle and mountain silver-flowers by using high-performance liquid chromatography. Background technique [0002] Honeysuckle and mountain silver flower are listed as two kinds of medicinal materials since the 2005 edition of "Pharmacopoeia of the People's Republic of China". Because the two original plant species are similar and the medicinal materials are similar in appearance and shape, it is difficult to distinguish them. Therefore, the sources of medicinal materials of honeysuckle and mountain silver flower are unclear and the varieties are confused. The phenomenon of pretending to be honeysuckle, but the material basis of the two is quite different, which directly affects the curative effect of t...
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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张芳于晓张永清刁兴彬郭庆梅李静王玲娜张龙霏
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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