Activation test for diagnosing heparin-induced thrombocytopenia

A technology of platelets and heparin, which is applied in the direction of disease diagnosis, biological testing, material inspection products, etc., can solve the problems of complex manual operations and the inability to perform functional test principles in an automatic way

Active Publication Date: 2018-12-18
SIEMENS HEALTHCARE DIAGNOSTICS PRODS
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Problems solved by technology

[0005] To date, both of the described functional test principles cannot be performed in conventional laboratories nor in

Method used

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  • Activation test for diagnosing heparin-induced thrombocytopenia

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example 1

[0043] Example 1: Homogeneous Immunoassay for Detection of Anti-PF4 / Heparin Complex Antibodies

[0044] used in this article The technique involves bringing latex particle-coupled chemiluminescent compounds (Chemibeads) and latex particle-coupled photosensitizers into proximity to each other by simultaneously binding them to the analyte so that singlet oxygen generated by the photosensitizer can stimulate the chemiluminescent compound.

[0045] 2 μL of heat-inactivated (heated at 56°C for 30 min) human plasma samples were mixed with 7.5 μL of a suspension containing washed human platelets from 6 healthy donors and 10 μL of buffer solution and incubated at 37°C (Approximately 300x10 9 Platelets / L), and the buffer solution contains 0.2IU / mL heparin. After 10 minutes, the following components were added to the reaction mixture:

[0046] 50 μL of "Chemibead" solution containing latex coated with a chemiluminescent compound (2-(4-(N,N,tetradecyl)-anilino-3-phenyldimethylthiophe...

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Abstract

The present invention relates to an activation test for diagnosing heparin-induced thrombocytopenia. More especially, the invention relates to determine functionality and an easy automation test of the heparin-induced thrombocytopenia (HIT). What is measured is the secretion of PF4 (platelet factor 4) from activated thrombocytes.

Description

technical field [0001] The present invention is in the field of in vitro diagnostics and relates to a functional, easily automatable test for the determination of heparin-induced thrombocytopenia. Background technique [0002] Heparin-induced thrombocytopenia (HIT) is a thrombotic disorder that may occur during heparin therapy and may result in life-threatening thromboembolic complications. Patients produce antibodies that bind to a complex of heparin and platelet factor 4 (PF4), known as anti-PF4 / heparin complex antibodies. In vivo, the antibody-bound PF4 / heparin complex binds to the platelet surface and causes activation of the platelet. This results in a decrease in platelet counts and an increased risk of thromboembolism. [0003] There are two main test principles used in the diagnosis of HIT. The first test principle is based on the direct detection of anti-PF4 / heparin complex antibodies in a patient's body fluid sample. To this end, the sample is contacted with a ...

Claims

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Application Information

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IPC IPC(8): G01N33/538
CPCG01N33/538G01N2800/226G01N33/6893G01N33/86G01N2800/222G01N33/564G01N2400/40G01N2333/522
Inventor 哈拉尔德·阿尔特豪斯格尔林德·克里斯特赫伯特·施瓦茨米夏埃拉·维克诺贝特·桑德尔
Owner SIEMENS HEALTHCARE DIAGNOSTICS PRODS
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