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Enzyme immunoassay plate and detection method thereof

A detection method and enzyme immunoassay technology, applied in the field of enzyme immunoassay, can solve the problems of cumbersome operation steps and low work efficiency, and achieve the effects of simplifying operation steps, improving work efficiency and improving accuracy

Pending Publication Date: 2018-12-18
SHANGHAI WEIYIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide an enzyme immunoassay plate and its detection method to solve the problem of low work efficiency caused by cumbersome operation steps of enzyme immunosorbent detection in the prior art

Method used

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  • Enzyme immunoassay plate and detection method thereof
  • Enzyme immunoassay plate and detection method thereof
  • Enzyme immunoassay plate and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] figure 1 , is a schematic structural diagram of an enzyme immunoassay plate provided in Example 1 of the present invention, figure 2 ,yes figure 1 cutaway view. Please refer to figure 1 and figure 2 , an enzyme immunoassay plate for enzyme immunoassay, comprising a microwell 12 plate body 10 made of polystyrene, one end of the microwell 12 plate body 10 is sunken inward for the first time to form a first concave Groove, the first groove has a first side wall 11 and a second side wall 18 oppositely arranged along its extending direction, and the first side wall 11 and the second side wall 18 are used to provide samples and detection reagents to enter The entrance of the enzyme immunoassay plate, the bottom surface of the first groove includes several recessed areas and non-recessed areas, and each of the recessed areas is recessed inward for the second time to form a microwell 12 whose bottom surface is parallel to the end surface , the microwells 12 are used to p...

Embodiment 2

[0052] The present invention also provides an enzyme immunoassay detection method, using the enzyme immunoassay plate described in Embodiment 1 of the present invention, at least including the following steps:

[0053] Step 1: Coating antigen or antibody in the microwell 12 of the enzyme immunoassay plate;

[0054] Step 2: Add the sample into the enzyme immunoassay plate, and the liquid surface of the sample is not over the cone top of the conical structure 14, and is located below the end surface, so as to ensure that the sample is consistent with the step 1 The antigen or antibody reacts fully, and the incubation time is set;

[0055] Step 3: Pour off the liquid in the microwell 12 after the treatment in step 2, and after cleaning, add the enzyme conjugate to the enzyme immunoassay plate, and the liquid level of the enzyme conjugate is submerged The conical top of the conical structure is located below the end face to ensure that the enzyme conjugate fully reacts with the s...

Embodiment 3

[0064] The present invention also provides a food IgG fourteen-item enzyme immunoassay single-serve kit for detecting food intolerance, using an enzyme immunoassay method described in Example 2 of the present invention to detect food intolerance Enzyme immunoassay of food IgG fourteen items.

[0065] The test kit includes:

[0066] (1) Embodiment one of the present invention Figure 5 and Figure 6 The enzyme immunoassay plate.

[0067] (2) Peripheral whole blood specimen, 1 vial, 50 μl.

[0068] (3) Sample diluent, 1 bottle, 5ml.

[0069] (4) Washing solution, 2 bottles, 5ml.

[0070] (5) Goat anti-human IgG enzyme conjugate, 1 bottle, 5ml.

[0071] (6) TMB developer, 1 bottle, 2.2ml.

[0072] The number of microwells 12 of the enzyme immunoassay plate is 16, and the microwells 12 are circular holes with a diameter of 6 mm, and their recessed depths are 3 mm, and the distance between the centers of two adjacent microwells 12 is 10mm. The coated antigens in each micro...

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Abstract

The invention provides an enzyme immunoassay plate and a detection method thereof for enzyme immunoassay. The enzyme immunoassay plate comprises a microwell plate main body, one end face of the microwell plate main body recesses inward for the first time to form a first groove, the first groove has a first side wall and a second side wall, which are arranged oppositely along an extension directionof the first groove, a bottom surface of the first groove comprises a plurality of recessed areas and non-recessed areas, each recessed area recesses inward for the second time to form a micropore inwhich the bottom surface is parallel to an end face, the adjacent micropores are isolated by the non-recessed areas, a conical structure is arranged on the non-recessed area between two adjacent micropores, each micropore is enclosed by the conical structure in the vicinity, or each micropore is enclosed by the conical structure in the vicinity and the side walls of the first groove, the conicaltop of the conical structure is located on a direction away from the non-recessed areas, the conical top is located below a first plane, and the first plane is a plane where the end face is located. The operation steps of the detection work are simplified, the enzyme immunoassay plate is suitable for use in primary medical units, and the working efficiency can be improved.

Description

technical field [0001] The invention belongs to the technical field of enzyme immunoassay, and relates to an enzyme immunoassay board and a detection method thereof. Background technique [0002] Enzyme immunosorbent assay is a detection technique with high sensitivity and good repeatability. The enzyme immunoassay in the prior art generally needs to be carried out on a polystyrene microporous plate, and each microwell serves as a food antigen or food antigen. The solid-phase carrier of the antibody is also a small immunobiochemical reactor. Enzyme immunoassay requires professionals to use a micropipette to add blood samples or sample diluents, washing solutions, Detection reagents such as enzyme conjugates and chromogenic reagents lead to time-consuming, increased workload, and reduced work efficiency. In primary medical units, when a blood sample needs to be tested for multiple tests and high-sensitivity tests for multiple blood samples are required at the same time , the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/573G01N33/531
CPCG01N33/531G01N33/54306G01N33/54393G01N33/573
Inventor 贺坚慧刘佳
Owner SHANGHAI WEIYIN BIOTECH CO LTD
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