A compound pharmaceutical composition with anti-lymphoma activity and its application

A composition, lymphoma technology, applied in the direction of drug combination, anti-tumor drug, organic active ingredients, etc., to achieve the effect of strong anti-proliferative activity, reduction of toxic and side effects, and good prospects for clinical drug development

Active Publication Date: 2020-01-07
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combination of chlorambucil and clinical drugs for the prevention and treatment of malignant tumor lymphoma has not yet been reported in the relevant literature almost no

Method used

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  • A compound pharmaceutical composition with anti-lymphoma activity and its application
  • A compound pharmaceutical composition with anti-lymphoma activity and its application
  • A compound pharmaceutical composition with anti-lymphoma activity and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Using Daudi cells, Raji cells and BC-3 cells to screen and optimize the compound pharmaceutical composition of chlorambucil CLB and genistein GEN

[0024] Take the cells in the logarithmic growth phase and inoculate 3×10 4 Cells / well were placed on a 96-well plate, and after 6 hours of growth, the supernatant was discarded by centrifugation, and then administered according to the following groups: Tumor cells were divided into no-drug group and drug-added group, and the drug-added group was set up with CLB and GEN alone. Group, CLB and GEN combined drug groups with different molar ratios, set 4-6 duplicate wells for each group, culture for 24 hours, discard the supernatant, add 100 μl of MTT (tetrazolium salt) serum-free culture medium containing 0.5 mg / ml for culture After 4 hours, add 100 μl of DMSO (dimethyl sulfoxide), place on a micro-oscillator to vibrate for 10 minutes, and then place on a microplate reader to detect the OD value at 570 nm. Results Th...

Embodiment 2

[0030] Embodiment 2: Analysis of the combined administration effect of CLB and GEN

[0031] Based on the Median-effect Principle (moderate effect principle or Chou-Talalay combined index method), the dose-response curve and the combined index curve (fa-C curve) under different effects were drawn using CombiDrug statistical software. The relationship between the effect and the combination index quantitatively evaluates whether there is a synergistic, antagonistic or additive relationship between the two drugs. Specific steps are as follows:

[0032] The drug effect is the inhibition rate (fa)=1-(the average OD570 value of the test group / the average OD570 of the tumor cell blank control group) according to the intermediate effect equation fa / fu=(D / Dm) m , take the logarithm logfa / fu=mlogD–mlogDm on both sides, set a=-mlogDm, b=m, x=logD, y=logfa / fu, and substitute into the intermediate effect equation to get y=bx–a; where fa is the drug effect , fu=1–fa, D is the drug concentr...

Embodiment 3

[0036] Example 3: Safety of CLB and GEN on peripheral blood mononuclear lymphocytes

[0037] The safety of CLB and GEN on PBMC cells was evaluated after isolation and culture of mononuclear lymphocytes from healthy volunteers ( figure 1): ① draw fresh blood from healthy volunteers into a heparin anticoagulant tube (sterile); then resuspend the cells (sterile) with an equal volume of PBS (or serum-free D-Hank buffer); ② put the suspended cells Add the pre-coated human lymphocyte separation medium (preheated at 37°C), the volume ratio of lymphatic separation medium to cell suspension is not less than 1:1; ③ 500×g (or 2000rpm) horizontal centrifuge for 20-30min, room temperature (20-30°C) at slow speed; ④ Discard the upper layer of plasma, carefully suck out the middle white mist layer and add it to 5ml (or 1-2 times the volume) of PBS (or serum-free cell culture buffer), 200×g or 1000rpm Centrifuge for 10 minutes at room temperature (20-30°C) at a slow speed, discard the supern...

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Abstract

The invention belongs to the field of natural medicines and discloses a compound medicinal composition with anti-lymphoma activity and its application. The active ingredient of the compound pharmaceutical composition is composed of chlorambucil and genistein in a molar ratio of 1:5-1:40. The combination of the two drugs has a strong synergistic effect on treating lymphoma.

Description

technical field [0001] The invention belongs to the field of natural medicines, in particular to a compound pharmaceutical composition with anti-lymphoma activity and its application. Background technique [0002] Cancer is a major chronic disease that seriously endangers human health, and has become the second largest killer after cardiovascular disease. Since the beginning of the 21st century, due to the rapid process of modern industrialization, environmental pollution and many other factors, the immune system of lymphocytes has been destroyed and the incidence of lymphoma has been increasing year by year. The drug system is very urgent. [0003] Nitrogen mustard compounds have definite anticancer activity, and are an earlier and important class of antineoplastic drugs. They play an important role in the treatment of tumors, especially hematological tumors, such as chronic lymphocytic leukemia, malignant lymphoid tumors and multiple myeloma. Chlorambucil is relatively ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/196A61K31/352A61P35/00
CPCA61K31/196A61K31/352A61P35/00A61K2300/00
Inventor 刘连陈建萍李雄刘腾彭咏波崔亮刘武昆谢国健
Owner FOSHAN UNIVERSITY
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