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A kind of rubber tree hbwrky82 gene and its application

A rubber tree, gene technology, applied in application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2021-03-16
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the studies on the function of WRKY transcription factors are focused on model plants such as Arabidopsis and rice or some economic herb crops, and there are relatively few reports on rubber trees.

Method used

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  • A kind of rubber tree hbwrky82 gene and its application
  • A kind of rubber tree hbwrky82 gene and its application
  • A kind of rubber tree hbwrky82 gene and its application

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0040] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention.

[0041] 1. Materials

[0042] The rubber tree materials used in this experiment were taken from the seedlings (Reyan 7-33-97) planted in the sixth team of the Chinese Academy of Tropical Agricultural Sciences test site and the budding seedlings (Reyan 7-33-97) of the fifth test site. Nicotiana tabacum L. (Nicotiana tabacum L.) (Nicotiana benthamiana) and wild-type Arabidopsis were provided by the Research Group on Mechanism of Dead Skin Prevention and Control.

[0043] The treatment of rubber tree leaves is to use five teams of budding seedlings with the same growth and stable stage leaves as samples for live treatment, and spray 0.4% The ET was treated and samples were taken at the same time point. At each time point, three leaves with the same growth on each seedling were used as a group, ...

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Abstract

The invention provides a rubber tree HbWRKY82 gene. The nucleotide sequence of the gene is as shown in SEQ ID NO:1. The invention further provides HbWRKY82 gene-coded protein and application of the gene. Accordingly, the HbWRKY82 gene is obtained through cloning in a rubber tree for the first time, the gene has the transcription factor function and is located in a cell nucleus, the expression of the gene is induced by ethephon, the expression is lowered in the leaf senescence process in a rubber tree with dead bark, and the reverse regulation function is achieved. After overexpression, tolerance of transgenic plants on stress environments such as high salt and drought; meanwhile, the function of regulating leaf senescence is achieved, and the important regulating effect is achieved in growth and development of the rubber tree and response to organism and microorganism adversity stress. In the future, the plants are subjected to genetic improvement by means of the HbWRKY82 gene, and effective gene resources can be provided for stress resistance such as plant salt resistance and drought resistance and delaying leaf senescence.

Description

technical field [0001] The invention relates to the field of biological technology and relates to a rubber tree HbWRKY82 gene and its application. Background technique [0002] As an irreplaceable industrial raw material and an important strategic resource, natural rubber has the characteristics of strong elasticity, good insulation, strong plasticity, airtightness of water and air insulation, and wear resistance, and is widely used in national defense, military industry, medicine, etc. important areas such as health and transportation. At present, the main source of natural rubber is Hevea brasiliensis, which is mainly grown in tropical regions such as Africa, Central America and Asia. As a non-traditional rubber planting area of ​​rubber trees in my country, the production of natural rubber is seriously affected by wind damage, drought, low temperature, cold and heat and other adversity stresses, which has higher requirements for the stress resistance of rubber tree varie...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82
CPCC07K14/415C12N15/8266C12N15/8273
Inventor 康桂娟闫栋陈晓丽曾日中杨礼富
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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