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Method for studying on uptake and transport mechanisms of nine active ingredients of Inula cappa extract in Caco-2 cell model

A technology of active ingredients and cell model, applied in the field of Chinese pharmacy research, can solve the problem that the pharmacokinetic process is not particularly clear and so on

Pending Publication Date: 2018-12-28
GUIZHOU MEDICAL UNIV
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Problems solved by technology

However, the in vivo pharmacokinetic process of the active ingredients of Goat Fructus is not particularly clear, especially the absorption and transport mechanism of the active ingredients of Goat Fructus has not been reported

Method used

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  • Method for studying on uptake and transport mechanisms of nine active ingredients of Inula cappa extract in Caco-2 cell model
  • Method for studying on uptake and transport mechanisms of nine active ingredients of Inula cappa extract in Caco-2 cell model
  • Method for studying on uptake and transport mechanisms of nine active ingredients of Inula cappa extract in Caco-2 cell model

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Experimental program
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Embodiment 1

[0018] 1 Materials and Instruments

[0019] 1.1 Materials

[0020] Caco-2 (American Type Culture Collection), 30-50 generations; DMEM, FBS, Penicillin / streptomycin and Trypsin-EDTA were all purchased from Thermo Fisher Scientific; HEPES buffer salt, RIPA and Rat tail tendon collagen type I were all purchased from Aladdin Industrial Corporation; BCA protein test kit (Solarbio); MTS (Promega); Sodium (Shanghai Lingjin Fine Chemical Co., Ltd.); Dimethyl sulfoxide (DMSO) (Tianjin Kemiou Chemical Reagent Co., Ltd.); AKP kit and LDH kit were purchased from Nanjing Jiancheng Technology Co., Ltd.; methanol, acetonitrile and formic acid (chromatographically pure); water is Watson's distilled water; the rest of the reagents are analytically pure.

[0021] Puerarin, luteolin, chlorogenic acid, and Verapamil (batch numbers: 110752-201514, 111720-201408, 110753-201415, 100223-200102) were all purchased from China National Institutes for Food and Drug Control; Tian Biotechnology Co., ...

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Abstract

The invention provides a method for studying on uptake and transport mechanisms of nine active ingredients of Inula cappa extract in a Caco-2 cell model. The method comprises the following steps: comparing spectrums of a control group cell liquid, a cell absorption sample, and Inula cappa active portion sample by adopting an ultra-high pressure liquid chromatography tandem high resolution mass spectrometer (UHPLC-Q-TOF MS / MS) and determining main ingredients which can enter the cells to be absorbed; then, establishing an uptake and transport model by using the Caco-2 cells; investigating the effects of drug concentration, pH, temperature, and P-gp inhibitor on the uptake and transport of the nine active ingredients of Inula cappa in the cell model; and then determining the uptake and transport mechanisms of the nine active ingredients of Inula cappa in the Caco-2 cell model.

Description

technical field [0001] The invention belongs to the research field of traditional Chinese medicine, and in particular relates to a research method for the uptake and transport mechanism of nine active ingredients in the extract of chrysanthemum chrysanthemum in a Caco-2 cell model. Background technique [0002] As the quintessence of the country, Chinese medicine has a history of thousands of years of application and has been well passed down. Its effectiveness and practicality must be beyond doubt. As we all know, traditional Chinese medicine itself is a complex system with multiple components, which makes its absorption and transport process in the body more complicated. However, modern research believes that "only the ingredients absorbed into the blood can be the effective ingredients of traditional Chinese medicine". Therefore, the study of the absorption and transport mechanism of traditional Chinese medicine is very important to uncover the "black box" of the internal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 巩仔鹏兰燕宇张楠李梅侯靖宇胡贺佳王永林李月婷陈思颖李勇军黄勇郑林王爱民陆苑刘亭马雪潘洁孙佳
Owner GUIZHOU MEDICAL UNIV
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