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Compound lactobacillus plantarum preparation with blood sugar reducing function and application of preparation

A technology of Lactobacillus plantarum and compound bacterial agents, applied in the direction of Lactobacillus, medical preparations containing active ingredients, applications, etc., can solve problems such as gastrointestinal dysfunction, liver and kidney function impairment, and visual abnormalities, and achieve good results. Effects on blood sugar, lowering blood sugar, and increasing body weight

Active Publication Date: 2019-01-04
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Clinically, long-term use of hypoglycemic drugs can cause severe side effects such as visual abnormalities, gastrointestinal dysfunction, liver and kidney dysfunction, etc.

Method used

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  • Compound lactobacillus plantarum preparation with blood sugar reducing function and application of preparation
  • Compound lactobacillus plantarum preparation with blood sugar reducing function and application of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the preparation of bacterial agent

[0030] 1. Preparation of bacterial agents

[0031] 1. Inoculate the test bacteria preserved in the glycerol tube into 10mL liquid MRS medium at an inoculum size of 2%, and culture it statically at 37°C for 16-18h.

[0032] 2. Inoculate the bacterial liquid obtained in step 1 into 10 mL of liquid MRS medium with an inoculum amount of 2%, and culture it statically at 37° C. for 16-18 hours.

[0033] 3. Inoculate the bacterial solution obtained in step 2 into 200 mL liquid MRS medium with an inoculum amount of 3%, and culture it statically at 37°C for 16-18 hours.

[0034] 4. Inoculate the bacterial solution obtained in step 3 into 5L liquid MRS culture medium with 3% inoculation amount, stir and ferment at 37°C and 120rpm for 16-18h (control pH6.5 during the fermentation process), then centrifuge at 4°C and 6000rpm for 10min , to collect the bacterial pellet.

[0035] 5. Take all the bacterial precipitates obtained in ...

Embodiment 2

[0048] Embodiment 2, the application of bacterial agent

[0049] 1. Preparation of bacterial solution

[0050] Dissolve every 1 g of the bacterium agent prepared in Example 2 in 0.85% sterile saline to obtain a diluted bacterium solution.

[0051] The SS18-37 bacterial agent prepared in Example 1 was used to obtain the SS18-37 bacterial liquid. In the SS18-37 bacterial liquid, the concentration of Lactobacillus plantarum SS18-37 was 1.0×10 9 CFU / mL.

[0052] The SS18-5 bacterial agent prepared in Example 1 was used to obtain the SS18-5 bacterial liquid. In the SS18-5 bacterial liquid, the concentration of Lactobacillus plantarum SS18-5 was 1.0×10 9 CFU / mL.

[0053] The SS18-37 / LGG composite bacterial agent prepared in Example 1 was used to obtain the SS18-37 / LGG bacterial liquid. In the SS18-37 / LGG bacterial solution, the concentration of Lactobacillus plantarum SS18-37 was 5.0×10 8 CFU / mL, the concentration of Lactobacillus rhamnosus LGG is 5.0×10 8 CFU / mL.

[0054] ...

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PUM

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Abstract

The invention discloses a compound lactobacillus plantarum preparation with a blood sugar reducing function and an application of the preparation. The compound bacterium consists of lactobacillus plantarum SS18-5 with the collection number of CGMCC No.14917 and lactobacillus plantarum SS18-37 with the collection number of CGMCC No.14918. In the compound bacterium, the CFU ratio of the lactobacillus plantarum SS18-5 to lactobacillus plantarum SS18-37 is 1:(0.01-100). The invention also discloses the application of the compound bacterium in preparation of a product. The product is used for treating and / or preventing diabetes. The preparation has the good blood sugar reducing effect on diabetes, can significantly improve insulin resistance, has protection effects on heart, liver, kidney, spleen and pancreas and has broad market application prospects.

Description

technical field [0001] The invention relates to a compound plant lactobacillus preparation with the function of lowering blood sugar and its application. Background technique [0002] Diabetes mellitus is a metabolic disease that threatens human health. Its notable feature is the chronic rise of blood sugar in patients, which in turn leads to metabolic disorders in the body. Among them, type II diabetes (T2D) is caused by insufficient secretion of insulin in the body, which leads to elevated blood sugar and insulin resistance, resulting in the inability of body cells to respond specifically after insulin is produced. T2D patients are often accompanied by a variety of complications, such as coronary heart disease, atherosclerosis, nephropathy, neuropathy, retinopathy, and foot lesions. [0003] Clinically, long-term use of hypoglycemic drugs can cause severe side effects such as visual abnormalities, gastrointestinal dysfunction, and liver and kidney dysfunction. Therefore,...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P3/10A23L33/135C12R1/25
CPCA61P3/10A23L33/135A61K35/747C12N1/20A23V2002/00A23V2400/169A23V2200/328
Inventor 郝红炜刘慧张红星谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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