Kit and detecting method for accurately measuring concentration of four immunosuppressant drugs in human whole blood
An immunosuppressant and accurate determination technology, which is applied in the field of medical testing, can solve the problems of difficult traceability of detection methods, and achieve the effects of small sample size, high throughput and high sensitivity
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Embodiment 1
[0069] A kit for determining the concentrations of four immunosuppressant drugs in human whole blood, including
[0070] Contains calibrators with six concentration gradients, and the calibrators of each concentration gradient are methanol solutions of tacrolimus, sirolimus, everolimus and cyclosporine A;
[0071] Among them, the six concentration points of tacrolimus, sirolimus, and everolimus are the same, in order: 2ng / mL, 5ng / mL, 10ng / mL, 20ng / mL, 50ng / mL, 100ng / mL; The six concentrations of sporomycin A are 10ng / mL, 50ng / mL, 200ng / mL, 500ng / mL, 1000ng / mL, 2000ng / mL;
[0072] Internal standard: internal standard solution is containing tacrolimus- 13 Cd 2 , sirolimus-d 3 , everolimus-d 4 and cyclosporine A-d 4 methanol solution, in which tacrolimus- 13 Cd 2 , sirolimus-d 3 and everolimus-d 4 The concentration in the internal standard solution is 10ng / mL; cyclosporine A-d 4 The concentration in the internal standard solution is 200ng / mL;
[0073] Quality control p...
Embodiment 2
[0094] A kit for determining the concentrations of four immunosuppressant drugs in human whole blood, including
[0095] Contains calibrators with six concentration gradients, and the calibrators of each concentration gradient are methanol solutions of tacrolimus, sirolimus, everolimus and cyclosporine A;
[0096] Among them, the six concentration points of tacrolimus, sirolimus, and everolimus are the same, in order: 2ng / mL, 5ng / mL, 10ng / mL, 20ng / mL, 50ng / mL, 100ng / mL; The six concentrations of sporomycin A are 10ng / mL, 50ng / mL, 200ng / mL, 500ng / mL, 1000ng / mL, 2000ng / mL;
[0097] Internal standard: internal standard solution is containing tacrolimus- 13 Cd 2 , sirolimus-d 3 , everolimus-d 4 and cyclosporine A-d 4 methanol solution, in which tacrolimus- 13 Cd 2 , sirolimus-d 3 and everolimus-d 4 The concentration in the internal standard solution is 10ng / mL; cyclosporine A-d 4 The concentration in the internal standard solution is 200ng / mL;
[0098] Quality control p...
Embodiment 3
[0106] A kit for determining the concentrations of four immunosuppressant drugs in human whole blood, including
[0107] Contains calibrators with six concentration gradients, and the calibrators of each concentration gradient are methanol solutions of tacrolimus, sirolimus, everolimus and cyclosporine A;
[0108] Among them, the six concentration points of tacrolimus, sirolimus, and everolimus are the same, in order: 2ng / mL, 5ng / mL, 10ng / mL, 20ng / mL, 50ng / mL, 100ng / mL; The six concentrations of sporomycin A are 10ng / mL, 50ng / mL, 200ng / mL, 500ng / mL, 1000ng / mL, 2000ng / mL;
[0109] Internal standard: internal standard solution is containing tacrolimus- 13 Cd 2 , sirolimus-d 3 , everolimus-d 4 and cyclosporine A-d 4 methanol solution, in which tacrolimus- 13 Cd 2 , sirolimus-d 3 and everolimus-d 4 The concentration in the internal standard solution is 10ng / mL; cyclosporine A-d 4 The concentration in the internal standard solution is 200ng / mL;
[0110] Quality control p...
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