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Kit and detection method for detecting D-dimer

A kit and dimer technology, applied in the field of chemical analysis and detection, can solve the problems of time-consuming, labor-intensive and complicated operation steps, and achieve the effects of saving detection time, simplifying the operation process, and reducing errors

Inactive Publication Date: 2019-01-11
GENRUI BIOTECH INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The embodiment of the present invention provides a kit for detecting D-dimer and its use method, which solves the technical problem of complex operation steps and time-consuming and labor-intensive detection of D-dimer

Method used

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  • Kit and detection method for detecting D-dimer
  • Kit and detection method for detecting D-dimer
  • Kit and detection method for detecting D-dimer

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Embodiment Construction

[0024] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0025] The kit for detecting D-dimer provided by the invention consists of antiserum, buffer solution, quality control product, magnetic card and stirring bar. Among them, the antiserum is composed of latex particles coupled with phosphate buffer saline and mouse anti-human D-dimer monoclonal antibody. The antiserum is used to generate antigen-antibody reaction with the antigen in the sample. The mass ratio of the latex particles coupled with the mouse anti-human D-dimer monoclonal antibody is (10-30): 1. In this embodiment, the preferred antiserum is composed of phosphate buffer solution and mouse anti-human D-dimer m...

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Abstract

The invention relates to the technical field of chemical analysis and detection and discloses a kit and detection method for detecting D-dimer. The kit is composed of antiserum, a buffer solution, a quality control product, a magnetic card and a stirrer, wherein the antiserum is composed of a phosphate buffer solution and mouse anti-human D-dimer monoclonal antibody coupled latex particles; the buffer solution is composed of the phosphate buffer solution and sodium chloride; the quality control product is a solution containing D-dimer; the mass ratio of the phosphate buffer solution to the mouse anti-human D-dimer monoclonal antibody coupled latex particles is (10-30):1; the mass ratio of the phosphate buffer solution to sodium chloride is 1:(2-3); the magnetic card is used for storing standard curve information of the kit; and the stirrer is used for stirring a to-be-detected sample. Preparing a required reagent on site and drawing a standard curve alone are not needed again when thekit is adopted to detect the D-dimer, so that not only is the detecting time saved, but also the labor force of detecting persons is reduced, in addition, the operation process of testing the D-dimeris simplified.

Description

technical field [0001] The invention relates to the technical field of chemical analysis and detection, in particular to a kit for detecting D-dimer and a detection method thereof. Background technique [0002] D-dimer (D-dimer) is one of the specific products of several multimers generated by the degradation of cross-linked fibrin after the action of plasmin. Under physiological conditions, a dynamic balance is maintained between plasmin and inhibitory enzymes in the normal body to ensure the timely formation and removal of fibrin, so that blood circulation can proceed normally. If this balance is disrupted, the intravascular coagulation tendency will be enhanced, fibrin aggregation, fibrin degradation products will increase, and the content of D-dimer will increase. Elevated levels of D-dimer, representing clot formation in the vascular circulation, are a sensitive marker of acute thrombosis. Therefore, the detection of D-dimer can reflect the thrombin and fibrinolytic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/536
CPCG01N33/536G01N33/54313
Inventor 朱漩汪建德
Owner GENRUI BIOTECH INC
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