Universal quality control product and quality control method for metabonomics detection

Abstract

The invention discloses a universal quality control product and a quality control method for metabonomics detection. The universal quality control product comprises a mixed standard sample of 4,4'-methylene bis(2-chloroaniline), p-anisidine, L-tyrosine methyl ester, 3-chloroaniline, 2, 4-dimethyl quinoline, sulfadiazine, atrazine, sulfadoxine, DL-leucine, N-benzoyl-L-tyrosine ethyl ester, 6-phenyl-2-thiouracil, N-(o-toluoyl) glycine, 2-methyl-5-nitroimidazole-1-ethanol, glycyrrhetinic acid, flavanone, epsilon-caprolactone and 2-aminopyridine. The 17 standard samples are derived from differentsubstance types, and are very stable, simple to prepare and small in usage amount. The quality control product can accurately reflect an instrument state of a chromatographic instrument or a mass spectrometer, and can successfully analyze instable reasons according to a spectrogram overlapping situation; and the judging accuracy reaches 100%.

Description

technical field [0001] The invention belongs to the technical field of mass sample detection of metabolomics, and in particular relates to a general quality control product for metabolomics detection and a quality control method thereof. Background technique [0002] Metabolomics is a discipline that studies the dynamic changes of biological endogenous small molecule metabolites before and after organisms are stimulated or disturbed by using chemical analysis techniques and quantitative methods, and determines the correlation between these changes and biological processes. [0003] Liquid chromatography-mass spectrometry is widely used in metabolomics detection due to its advantages of high throughput, high resolution, high sensitivity, suitable for detection of high boiling point, thermal instability and high molecular weight compounds. However, affected by factors such as the sample pre-processing method, the state of the instrument itself, and the environment, the instrum...

AI Technical Summary

Problems solved by technology

However, affected by factors such as the sample pre-processing method, the state of the instrument itself, and the environment, the instrument fluctuates during the data collection process. It is very important to judge whether the fluctuation of the instrument is within the normal range.

However, the existing technology needs to set up different quality control products for each experiment. The operation is cumbersome and unstable. The detection signals are easy to interfere with each other, the detection peaks overlap, or the response is not good. It is difficult to monitor accurately, or it is difficult to judge the experimental results. , and sometimes even cause instrument contamination, so how to provide a general quality control method that is efficient, fast, accurate, stable, does not require cumbersome repeated operations, and detects a large number of different types of samples at the same time, and can accurately determine the detection status of the instrument. Metabolomics Quality control method is a technical problem to be solved

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