Universal quality control product and quality control method for metabonomics detection

A technology of metabolomics and quality control methods, applied in measurement devices, scientific instruments, instruments, etc., can solve the problems of complicated operation, instrument pollution, instrument fluctuation, etc., and achieve the effect of less dosage, simple preparation and low cost

Inactive Publication Date: 2019-02-12
嘉兴迈维代谢生物科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, affected by factors such as the sample pre-processing method, the state of the instrument itself, and the environment, the instrument fluctuates during the data collection process. It is very important to judge whether the fluctuation of the instrument is within the normal range.
However, the existing technology needs to set up different quality control products for each experiment. The operation is cumbersome and unstable. The detection signals are easy to interfere with each other, the detection peaks overlap, or the response is not good. It is difficult to monitor accurately, or it is difficult to judge the experimental results. , and sometimes even cause instrument contamination, so how to provide a general quality control method that is efficient, fast, accurate, stable, does not require cumbersome repeated operations, and detects a large number of different types of samples at the same time, and can accurately determine the detection status of the instrument. Metabolomics Quality control method is a technical problem to be solved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Universal quality control product and quality control method for metabonomics detection
  • Universal quality control product and quality control method for metabonomics detection
  • Universal quality control product and quality control method for metabonomics detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The invention adopts ultra-high performance liquid chromatography-triple quadrupole / linear ion trap tandem mass spectrometry (UPLC-QTRAP) technology, the mass spectrometer is AB Sciex Q-TRAP 6500, and the analysis software is Multi Quant. The control product is composed of 17 mixed standards screened, and the 17 standard samples of the general quality control product of the present invention and their final concentrations for use are shown in Table 1:

[0032] Table 1

[0033]

[0034]

[0035]Through repeated tests, the present invention selects compounds with good separation, appropriate response, and stability as the mixed standard. The peak-out times of the 17 standard samples are basically evenly distributed between 0 and 11 minutes, and the mutual influence on each other's peak-out time is small, and the response is within 1 Between *10^6 and 3*10^7, it belongs to the best response range of the test instrument used. The 17 standard compounds have stable stru...

Embodiment 2

[0039] Use known samples of different states as standards to verify the monitoring accuracy of the quality control product of the present invention for different sample states and instrument states. Each known sample is used for repeated sampling, and each mixed standard quality control product is used for one time. Repeated sample injection, if the peak and overlapping situation of the known sample are consistent with the peak and overlapping situation of the mixed standard quality control product of the present invention, it means that the mixed standard quality control product of the present invention reflects accurately the situation of the sample and the state of the instrument. After repeated experimental verification, it was found that the mixed-standard quality control product of the present invention can 100% reflect the sample collection situation and the operating state of the instrument during sampling, and the collection of the sample to be tested can be reflected t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a universal quality control product and a quality control method for metabonomics detection. The universal quality control product comprises a mixed standard sample of 4,4'-methylene bis(2-chloroaniline), p-anisidine, L-tyrosine methyl ester, 3-chloroaniline, 2, 4-dimethyl quinoline, sulfadiazine, atrazine, sulfadoxine, DL-leucine, N-benzoyl-L-tyrosine ethyl ester, 6-phenyl-2-thiouracil, N-(o-toluoyl) glycine, 2-methyl-5-nitroimidazole-1-ethanol, glycyrrhetinic acid, flavanone, epsilon-caprolactone and 2-aminopyridine. The 17 standard samples are derived from differentsubstance types, and are very stable, simple to prepare and small in usage amount. The quality control product can accurately reflect an instrument state of a chromatographic instrument or a mass spectrometer, and can successfully analyze instable reasons according to a spectrogram overlapping situation; and the judging accuracy reaches 100%.

Description

technical field [0001] The invention belongs to the technical field of mass sample detection of metabolomics, and in particular relates to a general quality control product for metabolomics detection and a quality control method thereof. Background technique [0002] Metabolomics is a discipline that studies the dynamic changes of biological endogenous small molecule metabolites before and after organisms are stimulated or disturbed by using chemical analysis techniques and quantitative methods, and determines the correlation between these changes and biological processes. [0003] Liquid chromatography-mass spectrometry is widely used in metabolomics detection due to its advantages of high throughput, high resolution, high sensitivity, suitable for detection of high boiling point, thermal instability and high molecular weight compounds. However, affected by factors such as the sample pre-processing method, the state of the instrument itself, and the environment, the instrum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/7233G01N2030/027
Inventor 唐堂胡梦婷王宏郑彬
Owner 嘉兴迈维代谢生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap