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Method for extracting sweet tea element and ursolic acid from sweet tea

A technology of sweet tea and ursolic acid, applied in chemical instruments and methods, organic chemistry, steroids, etc., can solve the problems of wasting sweet tea resources, low utilization rate of functional components of sweet tea, etc. High efficiency of operation and purification

Active Publication Date: 2021-10-12
湖南食品药品职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Judging from the existing literature, the utilization rate of the functional components of sweet tea is not high, and they are treated as waste, which wastes a lot of sweet tea resources. If the functional components of sweet tea can be fully utilized, the production cost of its active components and the impact on the environment can be greatly reduced. protection of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Grind 100kg of dry sweet tea, add 300L of 80% ethanol aqueous solution, carry out reflux extraction at 80°C for 2 hours, and filter to obtain 260L of filtrate I and filter residue I; filtrate I is concentrated under reduced pressure to extract, add 30L of water to dissolve, filter to obtain filtrate II and Filter residue II; add 0.5 mol / L sodium hydroxide solution to filter residue II to 7.5, stir and dissolve, and filter to obtain filtrate III and filter residue III.

[0025] Mix the filter residue III with the filter residue I, add 8L of Aspergillus niger fermentation broth, ferment at room temperature for 4-6 days, then add 300L of 85% ethanol aqueous solution, carry out reflux extraction at 80°C for 2 hours, and filter to obtain 280L of filtrate IV and filter residue IV; filtrate IV Concentrate under reduced pressure to extract, add 35 L of water to dissolve, filter to obtain filtrate V and filter residue V; add 0.5 mol / L sodium hydroxide solution to filter residue V...

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Abstract

The invention provides a method for extracting sweet tea element and ursolic acid from sweet tea, including crushing, alcohol extraction, filtration, concentration, water dissolution, alkali dissolution, filtration, filter residue fermented by Aspergillus niger, alcohol extraction again, filtration, concentration, water dissolution , alkali-dissolved, respectively, column chromatography, elution, concentration under reduced pressure, crystallization and recrystallization once to obtain pure sweet tea and pure ursolic acid. The invention improves the stripping and transformation efficiency of its functional components through microbial transformation, and at the same time uses Aspergillus niger to produce acid to neutralize the alkali-treated product, which is also conducive to the rapid growth of Aspergillus niger; Separation of sweet tea and ursolic acid, high-purity products are obtained through column chromatography and crystallization, which effectively prevents sweet tea from being destroyed by alkaline reagents and improves its yield.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and mainly relates to a method for extracting sweet tea element and ursolic acid from sweet tea leaves. Background technique [0002] Sweet tea is a perennial vine of Rosaceae. It is a wild sweet plant unique to Guangxi. It is mainly produced in Dayao Mountain in our region. Because of its sweet taste, it is named sweet tea. Together with Mangosteen and Stevia, it is known as the three major sweet plants in Guangxi. It has a long history of application among the folks in our region. For a long time, local people have been drinking it as tea, and also used it as a substitute for sugar to process food, and it can also be used as medicine. In 1979, the Guangxi Analysis and Testing Research Center conducted an analysis of the chemical composition of sweet tea, and determined that the chemical structure of its sweetening substances was consistent with that of stevioside, and its swee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/08C07H15/256C07J63/00
CPCC07H1/08C07H15/256C07J63/008
Inventor 李雨嫣
Owner 湖南食品药品职业学院