A normal temperature mechanical perfusion system that can expand the source of liver donors

A mechanical and perfusate technology, applied in the field of mechanical perfusion systems at room temperature, can solve the problems of limiting the scope of materials, increasing the risk of rejection and infection, and achieving prevention of ischemia-reperfusion injury, good market prospects and social welfare. Value, the effect of expanding the source

Active Publication Date: 2021-06-25
嘉兴莱普晟医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the room temperature mechanical perfusion system combined with the corresponding perfusate can reduce the impact of warm ischemia-reperfusion injury on liver function to a certain extent and expand the source of liver donors. However, the above perfusion system still has many shortcomings, such as perfusion system Still need to use blood-containing perfusate for perfusion, because the blood needs to come from a donor, which also limits the scope of sampling
At the same time, the use of blood-containing perfusate will increase the risk of rejection and infection, and its tolerance to warm ischemia needs to be further improved

Method used

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  • A normal temperature mechanical perfusion system that can expand the source of liver donors
  • A normal temperature mechanical perfusion system that can expand the source of liver donors
  • A normal temperature mechanical perfusion system that can expand the source of liver donors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Composition and Connection Mode of Normal Temperature Mechanical Perfusion System Suitable for Liver Preservation

[0039] Such as figure 1 As shown, in the perfusion system (14), the central processing unit (1) is connected with the peristaltic pump (2), and the output power of the peristaltic pump 2 is controlled by the control unit (20) in the central processing unit (1), so The peristaltic pump (2) controls the perfusion pressure and flow rate of the perfusate through the change of the output power, and further, the peristaltic pump (2) is connected with the liver perfusate storage (3), and the liver perfusate storage (3) has two inner and outer layers , a cold and heat regulating tube is arranged between the inner and outer layers, and the cold and heat regulating tube is controlled by the control component (20) to adjust the temperature of the perfusate; the liver perfusate storage (3) is provided with a temperature sensor (16), Used to monitor the temp...

Embodiment 2

[0043] Example 2 Obtaining and Perfusion of Donor Liver

[0044] 1 experimental animal

[0045] 20-25 male Chinese miniature pigs, weighing 30±3Kg. All experimental animals were treated humanely and complied with the "Guidelines for the Management and Use of Experimental Animals" promulgated by the National Institutes of Health of the United States, and the experimental protocol was approved by the relevant experimental animal ethics committee.

[0046] 2. Method

[0047] (1) Experimental animals were fasted for 8 hours before operation. Anesthesia was induced by intramuscular injection of sodium pentobarbital, and body weight was weighed and recorded. Take the supine position and fix the limbs on the operating table, inhale high-flow oxygen, prepare the skin, connect the chest lead ECG monitoring, and connect the blood oxygen saturation probe to the end of the pigtail. Scalp needles were used to establish peripheral venous access through the marginal ear veins and fully f...

Embodiment 3

[0052] Example 3 Effect of naringenin-7-O-acetate on mechanically perfused liver preserved in vitro

[0053] Naringenin (naringenin) has antibacterial, anti-inflammatory, free radical scavenging, anti-oxidation, cough and expectorant, blood lipid lowering, anti-cancer and anti-tumor, antispasmodic and choleretic, prevention and treatment of liver disease, inhibition of platelet aggregation, anti-atherogenic artery However, whether it can be used to preserve the isolated liver, especially whether its derivative naringenin-7-O-acetate can be used as a component of the perfusion solution, which is beneficial to the mechanical perfusion preservation of the liver, is currently unknown . In order to explore the effect of naringenin-7-O-acetate on mechanically perfused isolated liver, two different liver perfusates were designed in this example, and the specific components are as described in Table 1:

[0054] Table 1 Composition of perfusate A and perfusate B (1000mL)

[0055] ...

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Abstract

The present invention provides a normal temperature mechanical perfusion system that can expand the source of liver donors. The mechanical perfusion system includes a central processing unit, a power unit, a liver storage unit, a liquid circulation unit, a monitoring unit and a liquid collection unit. The liquid circulation unit The assembly includes a liver perfusate reservoir, the liver perfusate reservoir stores liver perfusate, the liver perfusate contains naringenin derivatives and does not contain red blood cells.

Description

technical field [0001] The invention belongs to the technical field of medical equipment design and manufacture, and in particular relates to a normal temperature mechanical perfusion system capable of expanding the source of a liver donor. Background technique [0002] China is a country with a high incidence of liver disease. According to the "2013 China Health Statistical Yearbook" and "China Tumor Registration Annual Report", the annual number of new cases of viral hepatitis is 1.37 million, ranking first in Class A and B infectious diseases; the annual number of new cases of liver cancer is 355,000, and the annual death rate The rate of liver disease is as high as 26.26 / 100,000, ranking second in malignant tumors; the annual medical care expenses for liver disease are tens of billions of dollars, which has become a serious problem affecting national health, economic and social development. With the continuous improvement of liver transplantation techniques, perioperativ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226A01N1/0247
Inventor 李建辉杨子江郑俊
Owner 嘉兴莱普晟医疗科技有限公司
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