Storage solution for preserving rat liver homogenate s9 and preparation method thereof

A storage solution and evenly mixing technology, applied in the field of medicine, can solve the problems of reduced enzyme activity, unfavorable full utilization of rat liver homogenate S9, etc., to reduce expression, improve ischemia-reperfusion injury in rats, and promote liver regeneration. Effect

Active Publication Date: 2021-09-24
单纯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the storage of S9 at home and abroad is mainly stored in liquid nitrogen or -80°C refrigerator. However, in the process of specific use, repeated freezing and thawing will greatly reduce the activity of the enzyme, which is not conducive to the full utilization of S9 in rat liver homogenate.

Method used

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  • Storage solution for preserving rat liver homogenate s9 and preparation method thereof
  • Storage solution for preserving rat liver homogenate s9 and preparation method thereof
  • Storage solution for preserving rat liver homogenate s9 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 230mL glycerol, 350mL polyethylene glycol, 1mmol calcium chloride and 20mmol Tris-HCl, The balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 8000.

[0021] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.

Embodiment 2

[0023] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 200mL glycerol, 300mL polyethylene glycol, 0.5mmol calcium chloride and 25mmol Tris-HCl , the balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 7500.

[0024] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.

Embodiment 3

[0026] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 260mL glycerol, 400mL polyethylene glycol, 2mmol calcium chloride and 15mmol Tris-HCl, The balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 8500.

[0027] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.

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Abstract

The invention relates to a storage solution for preserving rat liver homogenate S9 and a preparation method thereof. In each 1L of the storage solution, the raw material components include: 200-260mL glycerol, 300-400mL polyethylene glycol, 0.5-2mmol calcium chloride and 15-25mmol Tris-HCl, the balance being water; the preparation method comprises the following steps: adding calcium chloride to the Tris-HCl buffer solution, adding other raw material components after mixing, stirring evenly, Obtain a stock solution. The storage solution for preserving rat liver homogenate S9 provided by the present invention can prolong the storage time of rat liver homogenate S9 under the premise of ensuring that the activity of rat liver homogenate S9 is basically unchanged, thereby avoiding the use of Repeated freezing and thawing affects its activity; and the preparation method of the storage solution for preserving rat liver homogenate S9 provided by the present invention is simple and the production cost is low.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a storage solution for preserving rat liver homogenate S9 and a preparation method thereof. Background technique [0002] Rat liver homogenate S9 is the mitochondria-free supernatant of liver homogenate, which contains a large number of drug-metabolizing enzymes such as CYPs, and is mainly used to study the metabolism of compounds and drug-drug interactions. At present, the storage of S9 at home and abroad is mainly stored in liquid nitrogen or -80°C refrigerator. However, in the process of specific use, repeated freezing and thawing will greatly reduce the activity of the enzyme, which is not conducive to the full utilization of S9 in rat liver homogenate. . Therefore, it is necessary to research and develop a new storage solution for preserving rat liver homogenate S9, so as to preserve rat liver homogenate S9 for a long time at a relatively high temperature, and to ensure t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 单纯崔生辉
Owner 单纯
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