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Stock solution for storing rat-liver homogenate S9 and preparation method thereof

A storage solution and uniform mixing technology, which is applied in the field of medicine, can solve the problems of unfavorable rat liver homogenate S9 full utilization and reduced enzyme activity, so as to improve the ischemia-reperfusion injury of rats, reduce the expression and reduce the production of inflammatory mediators Effect

Active Publication Date: 2017-06-20
单纯
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the storage of S9 at home and abroad is mainly stored in liquid nitrogen or -80°C refrigerator. However, in the process of specific use, repeated freezing and thawing will greatly reduce the activity of the enzyme, which is not conducive to the full utilization of S9 in rat liver homogenate.

Method used

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  • Stock solution for storing rat-liver homogenate S9 and preparation method thereof
  • Stock solution for storing rat-liver homogenate S9 and preparation method thereof
  • Stock solution for storing rat-liver homogenate S9 and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 230mL glycerol, 350mL polyethylene glycol, 1mmol calcium chloride and 20mmol Tris-HCl, The balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 8000.

[0021] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.

Embodiment 2

[0023] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 200mL glycerol, 300mL polyethylene glycol, 0.5mmol calcium chloride and 25mmol Tris-HCl , the balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 7500.

[0024] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.

Embodiment 3

[0026] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 260mL glycerol, 400mL polyethylene glycol, 2mmol calcium chloride and 15mmol Tris-HCl, The balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 8500.

[0027] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.

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Abstract

The invention relates to a stock solution for storing rat-liver homogenate S9 and a preparation method thereof. In every 1L of the stock solution, raw-material components comprise 200mL to 260mL of glycerol, 300mL to 400mL of polyethylene glycol, 0.5mmol to 2mmol of calcium chloride, 15mmol to 25mmol of Tris-HCl and the balance of water. The preparation method comprises the following steps of adding the calcium chloride into a Tris-HCl buffer solution, adding other raw-material components after uniformly mixing, then uniformly stirring so as to obtain the stock solution. The stock solution for storing the rat-liver homogenate S9, which is provided by the invention, can be used for prolonging the storage time of the rat-liver homogenate S9 on the premise that the activity of the rat-liver homogenate S9 is basically not changed; therefore, the repeated freezing and thawing to influence the activity of the rat-liver homogenate S9 in a use process is avoided; further, the preparation method of the stock solution for storing the rat-liver homogenate S9, which is provided by the invention, is simple; the production cost is low.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a storage solution for preserving rat liver homogenate S9 and a preparation method thereof. Background technique [0002] Rat liver homogenate S9 is the mitochondria-free supernatant of liver homogenate, which contains a large number of drug-metabolizing enzymes such as CYPs, and is mainly used to study the metabolism of compounds and drug-drug interactions. At present, the storage of S9 at home and abroad is mainly stored in liquid nitrogen or -80°C refrigerator. However, in the process of specific use, repeated freezing and thawing will greatly reduce the activity of the enzyme, which is not conducive to the full utilization of S9 in rat liver homogenate. . Therefore, it is necessary to research and develop a new storage solution for preserving rat liver homogenate S9, so as to preserve rat liver homogenate S9 for a long time at a relatively high temperature, and to ensure t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 单纯崔生辉
Owner 单纯
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