Stock solution for storing rat-liver homogenate S9 and preparation method thereof
A storage solution and uniform mixing technology, which is applied in the field of medicine, can solve the problems of unfavorable rat liver homogenate S9 full utilization and reduced enzyme activity, so as to improve the ischemia-reperfusion injury of rats, reduce the expression and reduce the production of inflammatory mediators Effect
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Embodiment 1
[0020] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 230mL glycerol, 350mL polyethylene glycol, 1mmol calcium chloride and 20mmol Tris-HCl, The balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 8000.
[0021] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.
Embodiment 2
[0023] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 200mL glycerol, 300mL polyethylene glycol, 0.5mmol calcium chloride and 25mmol Tris-HCl , the balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 7500.
[0024] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.
Embodiment 3
[0026] This example provides a storage solution for preserving rat liver homogenate S9, the raw material components are calculated by weight, including: 260mL glycerol, 400mL polyethylene glycol, 2mmol calcium chloride and 15mmol Tris-HCl, The balance is deionized water; wherein, the pH value of Tris-HCl is 7.4; the relative molecular mass of polyethylene glycol is 8500.
[0027] According to the above-mentioned raw materials, adopt the preparation method of the storage solution for preserving the rat liver homogenate S9 provided by the present invention to prepare the storage solution: add calcium chloride to Tris-HCl, and add other raw material components after mixing uniformly, Stir evenly to obtain a stock solution.
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