Method for inducing body cells of kidney beans into seedlings and application

A somatic cell, kidney bean technology, applied in application, horticultural methods, botanical equipment and methods, etc., to achieve the effect of shortening the creation time

Inactive Publication Date: 2019-03-29
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the induction of seedlings by somatic cells

Method used

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  • Method for inducing body cells of kidney beans into seedlings and application
  • Method for inducing body cells of kidney beans into seedlings and application
  • Method for inducing body cells of kidney beans into seedlings and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Induction method of somatic cell seedlings of kidney bean "Shuangfeng No. 1"

[0039] (1) Disinfection and activation of seeds: First, soak the beans in 30% (w / w) sodium hypochlorite, rinse for 30 minutes; then rinse with sterile water for 2 minutes each time, and finally soak them in sterile water for 15 hours;

[0040] (2) Hypocotyl stripping: the entire hypocotyl of the kidney bean is cut in a sterile environment;

[0041] (3) Osmotic pressure treatment: hypocotyls were placed in osmotic pressure solution for 10 hours. Osmotic fluid refers to: 5% sucrose + 1 mg / mL6-BA + 20 mg / mL adenine;

[0042](4) Embryoid body induction: Hypocotyls treated with osmotic pressure were cultured in induction medium No. 1 for 20 days. Cultured in induction medium No. 2 for 1 day to induce embryoid bodies;

[0043] Induction medium No. 1 refers to: MS medium + 1 mg / mL6-BA + 20 mg / mL adenine + 1 mg / mL allantoin;

[0044] Induction medium No. 2 refers to: MS medium + 1mg / mL6-BA + 20 m...

Embodiment 2

[0047] Method for Inducing Somatic Seedling Formation of Kidney Bean "Feng Kidou No. 9"

[0048] (1) Disinfection and activation of seeds: First, soak the beans in 30% (w / w) sodium hypochlorite, rinse for 30 minutes; then rinse with sterile water for 2 minutes each time, and finally soak them in sterile water for 15 hours;

[0049] (2) Hypocotyl stripping: the entire hypocotyl of the kidney bean is cut in a sterile environment;

[0050] (3) Osmotic pressure treatment: hypocotyls were placed in osmotic pressure solution for 10 hours. Osmotic fluid refers to: 5% sucrose + 1 mg / mL6-BA + 20 mg / mL adenine;

[0051] (4) Embryoid body induction: Hypocotyls treated with osmotic pressure were placed in induction medium No. 1 and cultured for 30 days, the growth point was cut off, put back into induction medium No. 1 and cultured for 10 days, and then transferred to induction culture Cultured in Base 2 for 1 day to induce embryoid bodies;

[0052] Induction medium No. 1 refers to: MS...

Embodiment 3

[0060]

[0061] in conclusion:

[0062] (1) The present invention breaks through the bottleneck that the prior art cannot use somatic cells as precursors to induce seedlings.

[0063] (2) Construct a set of somatic cell induced seedling test system for common beans. Using this test system as a test platform can realize the screening and innovation of kidney bean resources in the laboratory, which can greatly shorten the creation time of kidney bean resources, and further develop new varieties of kidney beans. Breeding guarantees.

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Abstract

The invention discloses a method for inducing body cells of kidney beans into seedlings. The method comprises the following steps: disinfecting and activating seeds, stripping the whole hypocotyl, treating the hypocotyl by an osmotic pressure, inoculating the hypocotyl into an induction medium 1 for 5-30 days, cutting off the hypocotyl growing point, transferring into the induction medium 1 for 5-30 days, then transferring into an induction medium 2, transferring into the induction medium 1 within 1-10 days for 5-30 days, then transferring into an induction medium 3 to be cultured for 10-30 days, and inducing a process of obtaining normal kidney bean seedlings. The method is a standard kidney bean embryoid induction and seedling growth system. The method can be used for solving the problemthat the body cells of kidney beans cannot be rapidly induced into seedlings, establishes a test system for rapidly inducing the body cells of the kidney beans into seedlings, and provides a reliabletest platform for germplasm resource innovation of the kidney beans.

Description

technical field [0001] The invention belongs to the technical field of application of biotechnology in vegetable breeding, and relates to a general method for inducing somatic bean seedlings by using tissue culture technology. Background technique [0002] Kidney beans for pods have a long history of cultivation in my country, with a wide range of cultivation and a large cultivation area. According to the FAO report, in 2014, the total cultivated area of ​​vegetables in my country was about 374 million mu, of which the planting area of ​​pod beans was about 9.5308 million mu, an increase of 67,700 mu compared with 2013. It is one of the bulk vegetable crops. important place in life. In addition, my country's bean cultivation area and total output rank first in the world, which plays a decisive role in the world's vegetable production and development. [0003] Germplasm resources are an important material basis for new breed selection and genetic breeding research. The coll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 古瑜刘艳军韩启厚于海龙
Owner TIANJIN RES INST OF VEGETABLE
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