Method for inducing body cells of kidney beans into seedlings and application
A somatic cell, kidney bean technology, applied in application, horticultural methods, botanical equipment and methods, etc., to achieve the effect of shortening the creation time
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Embodiment 1
[0038] Induction method of somatic cell seedlings of kidney bean "Shuangfeng No. 1"
[0039] (1) Disinfection and activation of seeds: First, soak the beans in 30% (w / w) sodium hypochlorite, rinse for 30 minutes; then rinse with sterile water for 2 minutes each time, and finally soak them in sterile water for 15 hours;
[0040] (2) Hypocotyl stripping: the entire hypocotyl of the kidney bean is cut in a sterile environment;
[0041] (3) Osmotic pressure treatment: hypocotyls were placed in osmotic pressure solution for 10 hours. Osmotic fluid refers to: 5% sucrose + 1 mg / mL6-BA + 20 mg / mL adenine;
[0042](4) Embryoid body induction: Hypocotyls treated with osmotic pressure were cultured in induction medium No. 1 for 20 days. Cultured in induction medium No. 2 for 1 day to induce embryoid bodies;
[0043] Induction medium No. 1 refers to: MS medium + 1 mg / mL6-BA + 20 mg / mL adenine + 1 mg / mL allantoin;
[0044] Induction medium No. 2 refers to: MS medium + 1mg / mL6-BA + 20 m...
Embodiment 2
[0047] Method for Inducing Somatic Seedling Formation of Kidney Bean "Feng Kidou No. 9"
[0048] (1) Disinfection and activation of seeds: First, soak the beans in 30% (w / w) sodium hypochlorite, rinse for 30 minutes; then rinse with sterile water for 2 minutes each time, and finally soak them in sterile water for 15 hours;
[0049] (2) Hypocotyl stripping: the entire hypocotyl of the kidney bean is cut in a sterile environment;
[0050] (3) Osmotic pressure treatment: hypocotyls were placed in osmotic pressure solution for 10 hours. Osmotic fluid refers to: 5% sucrose + 1 mg / mL6-BA + 20 mg / mL adenine;
[0051] (4) Embryoid body induction: Hypocotyls treated with osmotic pressure were placed in induction medium No. 1 and cultured for 30 days, the growth point was cut off, put back into induction medium No. 1 and cultured for 10 days, and then transferred to induction culture Cultured in Base 2 for 1 day to induce embryoid bodies;
[0052] Induction medium No. 1 refers to: MS...
Embodiment 3
[0060]
[0061] in conclusion:
[0062] (1) The present invention breaks through the bottleneck that the prior art cannot use somatic cells as precursors to induce seedlings.
[0063] (2) Construct a set of somatic cell induced seedling test system for common beans. Using this test system as a test platform can realize the screening and innovation of kidney bean resources in the laboratory, which can greatly shorten the creation time of kidney bean resources, and further develop new varieties of kidney beans. Breeding guarantees.
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