The Manshan Red SSR primer based on the transcription group sequencing method and screening method and application
A technology of transcriptome sequencing and Manshanhong, which is applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., to achieve the effect of fast method, high versatility and practicability
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Embodiment 1
[0037] [Example 1] Development of EST-SSR primers of Manshanhong with high polymorphism
[0038] Based on the high-throughput sequencing of transcriptome provided by the present invention, combined with bioinformatics methods for SSR sequence search and SSR marker primer design and verification, the specific implementation methods are as follows:
[0039] (1) Screening of Unigene sequences rich in SSR sites: The petal materials in the full-blooming stage of Mangosaurus were selected to extract total RNA, and a transcriptome sequencing library was constructed, and high-throughput sequencing was performed using Illumina next-generation sequencer. Strictly filter the sequencing data and assemble the transcriptome data to obtain Unigene, which is used as the background data for the development of SSR markers. Use MISA software to search for SSR sites on Unigene sequences. The search criteria are: dinucleotides, trinucleotides, tetranucleotides, pentanucleotides, and hexanucleotide...
Embodiment 2
[0045] [Example 2] Application of 25 pairs of EST-SSR primers in the study of genetic diversity of Manshanhong population
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