Method for rapid production of root-knot nematode perineal pattern

Abstract

The invention discloses a method for quickly producing root-knot nematode perineal patterns, which comprises the following steps: (1) picking and transferring females; (2) using a No. 11 scalpel to obliquely cut off the complete perineum with the female tail One-fifth to one-half of the worm body of the pattern, and then transfer the part of the worm body with the perineal pattern to 60% lactic acid solution by mass fraction; (3) cleaning: make the incision downward or to the side, and use an insect needle Press the epidermis of the worm body 1 to 5 times, and the contents in the worm body will be released quickly, and the contents can be cleaned up in 10 to 60 seconds; (4) Use a No. 11 scalpel to cut along the edge of the pattern to obtain a worm with a complete perineal pattern Small pieces of epidermis; (5) Making permanent slides with root-knot nematode perineal patterns. The present invention uses an optimized cutting method, which can cut the perineal pattern part in one piece, and the cross-sectional area obtained by oblique cutting is large, and the content is directly cleaned on the large epidermis, and the operation is more convenient and convenient. The method of the invention is short in time, high in success rate, and the skin is not easily damaged during cleaning.

Description

technical field [0001] The invention relates to a method for making permanent slides with perineal patterns of root-knot nematodes. Background technique [0002] With the integration of the global economy and the increasing international trade, the risk of alien quarantine pests being introduced into my country is becoming more and more serious. my country has become one of the countries most seriously threatened by alien quarantine pests in the world. Biological invasions have attracted the attention of governments all over the world due to their social, economic and ecological problems. These invasive species not only seriously threaten the ecosystem, accelerate species extinction, but also cause the loss of biodiversity. [0003] At present, there are 20 species (genera) of quarantine nematodes included in the list of quarantine pests for imported plants in my country, among which root-knot genus (non-Chinese species) is quarantine nematodes. Root-knot nematodes have the...

AI Technical Summary

Problems solved by technology

[0025] According to the literature, inserting a needle to remove the contents of the worm body is very difficult, and it is difficult to clean the contents

And, according to figure 2 The cutting surface is very small, and it is not easy to clean the contents. Moreover, this cutting method is also difficult to operate. It cannot be cut with one knife. It has to turn 90 degrees in the middle, which is very difficult to operate.

[0026] Therefore, the above-mentioned perineal pattern slicing methods have defects, and the operation is difficult, which is not conducive to the operation of laboratory appraisal personnel.

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